Unraveling the mode of function of the W-containing formate dehydrogenase (Fdh) enzyme

• Autor(a) principal: Alves, Guilherme Vilela
• Data de Publicação: 2022
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10362/142774
• topic: Formate Dehydrogenase (Fdh); CO2 Enzymatic Reduction; Mo- and W-Enzymes; Desulfovibrio vulgaris; X-ray Crystallography; Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias

Anthropogenic CO2 emissions are rising since the Industrial Revolution and, with them, Earth temperature, as its consequences already impact our society, these being a 21st century key challenge. Harnessing millions of years of evolution is a promising way to halt CO2 emissions and possibly revert them. Formate Dehydrogenases (Fdh), especially the Mo and W-de-pendent ones, can efficiently, specifically and reversibly interconvert formate and CO2. Additionally, these enzymes are also relevant in human health with formate being a key metabolite in several diseases. Here, we report the structural characterization of the Desulfovibrio vulgaris FdhAB mutants and in complex with ligands. The results here reported push us one small step closer to an Fdh-based biotechnological solution capable of driving our society towards equilibrium with Nature.