Evaluation of lipopolysaccharide aggregation by light scattering spectroscopy

Detalhes bibliográficos
Autor(a) principal: Santos, Nuno C.
Data de Publicação: 2003
Outros Autores: Silva, Ana C., Castanho, Miguel A. R. B., Martins-Silva, João, Saldanha, Carlota
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/17631
Resumo: © 2003 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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spelling Evaluation of lipopolysaccharide aggregation by light scattering spectroscopyAggregationBacteriaLight scattering spectroscopyLipopolysaccharideSupramolecular chemistry© 2003 WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimLipopolysaccharides (LPS) are cell wall components of Gramnegative bacteria. These molecules behave as bacterial endotoxins and their release into the bloodstream is a determinant of the development of a wide range of pathologies. These amphipathic molecules can self-aggregate into supramolecular structures with different shapes and sizes. The formation of these structures occurs when the LPS concentration is higher than the apparent critical micelle concentration (CMCa). Light scattering spectroscopy (both static and dynamic) was used to directly characterize the aggregation process of LPS from Escherichia coli serotype 026:B6. The results point to a CMCa value of 14μgmL-1 and the existence of premicelle LPS oligomers below this concentration. Both structures were characterized in terms of molecular weight (5.5 x 106 and 16 x 106 gmol 1 below and above the CMCa , respectively), interaction with the aqueous environment, gyration radius (56 and 105 nm), hydrodynamic radius, (60 and 95 nm) and geometry of the supramolecular structures (nearly spherical). Our data indicates that future in vitro experiments should be carried out both below and above the CMCa. The search for drugs that interact with the aggregates, and thus change the CMCa and condition LPS interactions in the bloodstream, could be a new way to prevent certain bacterial-endotoxin-related pathologies.This work was supported by Fundação para a Ciência e Tecnologia and by Fundação Calouste Gulbenkian (Portugal).Repositório da Universidade de LisboaSantos, Nuno C.Silva, Ana C.Castanho, Miguel A. R. B.Martins-Silva, JoãoSaldanha, Carlota2015-03-05T15:18:54Z20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/17631engChemBioChem 2003, 4, 96-1001439-4227http://dx.doi.org/10.1002/cbic.200390020metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:22:11Zoai:repositorio.ul.pt:10451/17631Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:22:11Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evaluation of lipopolysaccharide aggregation by light scattering spectroscopy
title Evaluation of lipopolysaccharide aggregation by light scattering spectroscopy
spellingShingle Evaluation of lipopolysaccharide aggregation by light scattering spectroscopy
Santos, Nuno C.
Aggregation
Bacteria
Light scattering spectroscopy
Lipopolysaccharide
Supramolecular chemistry
title_short Evaluation of lipopolysaccharide aggregation by light scattering spectroscopy
title_full Evaluation of lipopolysaccharide aggregation by light scattering spectroscopy
title_fullStr Evaluation of lipopolysaccharide aggregation by light scattering spectroscopy
title_full_unstemmed Evaluation of lipopolysaccharide aggregation by light scattering spectroscopy
title_sort Evaluation of lipopolysaccharide aggregation by light scattering spectroscopy
author Santos, Nuno C.
author_facet Santos, Nuno C.
Silva, Ana C.
Castanho, Miguel A. R. B.
Martins-Silva, João
Saldanha, Carlota
author_role author
author2 Silva, Ana C.
Castanho, Miguel A. R. B.
Martins-Silva, João
Saldanha, Carlota
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Santos, Nuno C.
Silva, Ana C.
Castanho, Miguel A. R. B.
Martins-Silva, João
Saldanha, Carlota
dc.subject.por.fl_str_mv Aggregation
Bacteria
Light scattering spectroscopy
Lipopolysaccharide
Supramolecular chemistry
topic Aggregation
Bacteria
Light scattering spectroscopy
Lipopolysaccharide
Supramolecular chemistry
description © 2003 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01T00:00:00Z
2015-03-05T15:18:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/17631
url http://hdl.handle.net/10451/17631
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv ChemBioChem 2003, 4, 96-100
1439-4227
http://dx.doi.org/10.1002/cbic.200390020
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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