Leishmania infantum infection of primary human myeloid cells

• Autor(a) principal: Picard, Morgane
• Data de Publicação: 2022
• Outros Autores: Soundaramourty, Calaiselvy, Silvestre, Ricardo, Estaquier, Jérôme, André, Sónia
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: https://hdl.handle.net/1822/80016
• topic: Leishmania; flow cytometry; blood; monocyte; neutrophil; Ciências Médicas::Medicina Básica; Ciências Médicas::Biotecnologia Médica; Science & Technology

Circulating phagocytic cells often serve as cellular targets for a large number of pathogens such as <i>Leishmania</i> parasites. Studying primary human cells in an infectious context requires lengthy procedures for cell isolation that may affect the analysis performed. Using whole blood and a no-lyse and no-wash flow cytometric assay (<i>NoNo</i> assay), we monitored the <i>Leishmania infantum</i> infection of primary human cells. We demonstrated, using fluorescent parasites, that among monocyte cell populations, <i>L. infantum</i> preferentially infects classical (CD14⁺CD16⁻) and intermediate (CD14⁺CD16⁺) primary human monocytes in whole blood. Because classical monocytes are the preponderant population, they represent the larger <i>L. infantum</i> reservoir. Moreover, we also found that, concomitantly to monocyte infection, a subset of PMNs is infected early in whole blood. Of interest, in whole blood, PMNs are less infected compared to classical monocytes. Overall, by using this <i>NoNo</i> assay, we provided a novel avenue in our understanding of host–leishmania interactions.