Virulence shifts of Candida albicans biofilms induced by ethinylestradiol/drospirenone contraceptive
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://actafarmaceuticaportuguesa.com/index.php/afp/article/view/329 |
Resumo: | It is estimated that approximately 167 million women worldwide use combined oral contraceptive (COC). In United States, products containing ethinylestradiol plus drospirenone (EE+DRSP) moved about US$ 616 million in 2008. There is evidence that contraceptives increase the virulence of Candida albicans. However, modulation of fungal virulence by drospirenone-containing COC remains unknown. The objective of this study was to evaluate whether virulence factors are increased when C. albicans is exposed to EE+DRSP. The ATCC®90028™, SC5314 and 15A2 strains were grown in the presence of EE+DRSP at 10 nM:10 μM, 50 nM:50 μM, and 100 nM:100 μM under continuous flow in a dedicated bioreactor for biofilm growth. 72-h biofilms were evaluated in relation to their proteolytic capacities, biomass production (MTT reduction assay), and induction of germ-tube formation and measurements of agar invasion. Only 50 nM:50 μM EE+DRSP caused reductions in biomass (p=0.023); the other did not promote any change (p>0.05). The contraceptive exposure was insufficient to cause any increase in germ tubes induction (p>0.05). There was a concentration-dependent increment in the hyphae invasiveness. On the other hand, the proteolytic activity increased in a concentration- dependent manner (p<0.01). When exposed to EE+DRSP, Candida albicans strains do not form greater amounts of biofilm, neither generate more germ tubes, nor become more invasive; however, they secrete greater quantities of proteases, which facilitate the adhesion and invasion of tissue. |
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Virulence shifts of Candida albicans biofilms induced by ethinylestradiol/drospirenone contraceptiveIt is estimated that approximately 167 million women worldwide use combined oral contraceptive (COC). In United States, products containing ethinylestradiol plus drospirenone (EE+DRSP) moved about US$ 616 million in 2008. There is evidence that contraceptives increase the virulence of Candida albicans. However, modulation of fungal virulence by drospirenone-containing COC remains unknown. The objective of this study was to evaluate whether virulence factors are increased when C. albicans is exposed to EE+DRSP. The ATCC®90028™, SC5314 and 15A2 strains were grown in the presence of EE+DRSP at 10 nM:10 μM, 50 nM:50 μM, and 100 nM:100 μM under continuous flow in a dedicated bioreactor for biofilm growth. 72-h biofilms were evaluated in relation to their proteolytic capacities, biomass production (MTT reduction assay), and induction of germ-tube formation and measurements of agar invasion. Only 50 nM:50 μM EE+DRSP caused reductions in biomass (p=0.023); the other did not promote any change (p>0.05). The contraceptive exposure was insufficient to cause any increase in germ tubes induction (p>0.05). There was a concentration-dependent increment in the hyphae invasiveness. On the other hand, the proteolytic activity increased in a concentration- dependent manner (p<0.01). When exposed to EE+DRSP, Candida albicans strains do not form greater amounts of biofilm, neither generate more germ tubes, nor become more invasive; however, they secrete greater quantities of proteases, which facilitate the adhesion and invasion of tissue.Acta Farmacêutica Portuguesa2022-12-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://actafarmaceuticaportuguesa.com/index.php/afp/article/view/329Acta Farmacêutica Portuguesa; v. 11 n. 2 (2022): Acta Farmacêutica Portuguesa; 61-692182-3340reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPporhttps://actafarmaceuticaportuguesa.com/index.php/afp/article/view/329https://actafarmaceuticaportuguesa.com/index.php/afp/article/view/329/258Rosa, EdvaldoRymovicz, AlinneSelow, MarcelaOliveira, NicolyRosa, RosimeireCampelo, PatríciaBianchini, LuizHardy, Anainfo:eu-repo/semantics/openAccess2022-12-30T17:00:27Zoai:ojs.actafarmaceuticaportuguesa.com:article/329Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:29:07.070377Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Virulence shifts of Candida albicans biofilms induced by ethinylestradiol/drospirenone contraceptive |
title |
Virulence shifts of Candida albicans biofilms induced by ethinylestradiol/drospirenone contraceptive |
spellingShingle |
Virulence shifts of Candida albicans biofilms induced by ethinylestradiol/drospirenone contraceptive Rosa, Edvaldo |
title_short |
Virulence shifts of Candida albicans biofilms induced by ethinylestradiol/drospirenone contraceptive |
title_full |
Virulence shifts of Candida albicans biofilms induced by ethinylestradiol/drospirenone contraceptive |
title_fullStr |
Virulence shifts of Candida albicans biofilms induced by ethinylestradiol/drospirenone contraceptive |
title_full_unstemmed |
Virulence shifts of Candida albicans biofilms induced by ethinylestradiol/drospirenone contraceptive |
title_sort |
Virulence shifts of Candida albicans biofilms induced by ethinylestradiol/drospirenone contraceptive |
author |
Rosa, Edvaldo |
author_facet |
Rosa, Edvaldo Rymovicz, Alinne Selow, Marcela Oliveira, Nicoly Rosa, Rosimeire Campelo, Patrícia Bianchini, Luiz Hardy, Ana |
author_role |
author |
author2 |
Rymovicz, Alinne Selow, Marcela Oliveira, Nicoly Rosa, Rosimeire Campelo, Patrícia Bianchini, Luiz Hardy, Ana |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Rosa, Edvaldo Rymovicz, Alinne Selow, Marcela Oliveira, Nicoly Rosa, Rosimeire Campelo, Patrícia Bianchini, Luiz Hardy, Ana |
description |
It is estimated that approximately 167 million women worldwide use combined oral contraceptive (COC). In United States, products containing ethinylestradiol plus drospirenone (EE+DRSP) moved about US$ 616 million in 2008. There is evidence that contraceptives increase the virulence of Candida albicans. However, modulation of fungal virulence by drospirenone-containing COC remains unknown. The objective of this study was to evaluate whether virulence factors are increased when C. albicans is exposed to EE+DRSP. The ATCC®90028™, SC5314 and 15A2 strains were grown in the presence of EE+DRSP at 10 nM:10 μM, 50 nM:50 μM, and 100 nM:100 μM under continuous flow in a dedicated bioreactor for biofilm growth. 72-h biofilms were evaluated in relation to their proteolytic capacities, biomass production (MTT reduction assay), and induction of germ-tube formation and measurements of agar invasion. Only 50 nM:50 μM EE+DRSP caused reductions in biomass (p=0.023); the other did not promote any change (p>0.05). The contraceptive exposure was insufficient to cause any increase in germ tubes induction (p>0.05). There was a concentration-dependent increment in the hyphae invasiveness. On the other hand, the proteolytic activity increased in a concentration- dependent manner (p<0.01). When exposed to EE+DRSP, Candida albicans strains do not form greater amounts of biofilm, neither generate more germ tubes, nor become more invasive; however, they secrete greater quantities of proteases, which facilitate the adhesion and invasion of tissue. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-24 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://actafarmaceuticaportuguesa.com/index.php/afp/article/view/329 |
url |
https://actafarmaceuticaportuguesa.com/index.php/afp/article/view/329 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://actafarmaceuticaportuguesa.com/index.php/afp/article/view/329 https://actafarmaceuticaportuguesa.com/index.php/afp/article/view/329/258 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Acta Farmacêutica Portuguesa |
publisher.none.fl_str_mv |
Acta Farmacêutica Portuguesa |
dc.source.none.fl_str_mv |
Acta Farmacêutica Portuguesa; v. 11 n. 2 (2022): Acta Farmacêutica Portuguesa; 61-69 2182-3340 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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