Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/75131 |
Resumo: | Engineering complex tissues requires the use of advanced biofabrication techniques that allow the replication of the tissue's 3D microenvironment, architecture and cellular interactions. In the case of skin, the most successful strategies to introduce the complexity of hair follicle (HF) appendages have highlighted the importance of facilitating direct interaction between dermal papilla (DP) cells and keratinocytes (KCs) in organotypic skin models. In this work, we took advantage of microscopy-guided laser ablation (MGLA) to microfabricate a fibroblast-populated collagen hydrogel and create a subcompartment that guides the migration of KCs and lead their interaction with DP cells to recreate follicular structures. Upon definition of the processing parameters (laser incidence area and power), MGLA was used to create 3D microchannels from the surface of a standard organotypic human skin model up to the aggregates containing DP cells and KCs, previously incorporated into the dermal-like fibroblast-collagen layer. Analysis of the constructs showed that the fabricated microfeatures successfully guided the fusion between epidermal and aggregates keratinocytes, which differentiated into follicular-like structures within the organotypic human skin model, increasing its functionality. In summary, we demonstrate the fabrication of a highly structured 3D hydrogel-based construct using MGLA to attain a complex skin model bearing folliculoid structures, highlighting its potential use as an in vitro platform to study the mechanisms controlling HF development or for the screening of bioactive substances. |
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Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendageshair follicleIn Vitro ModelLaser ablation3D tissue modelbiofabricationdermal papillaskin modelEngenharia e Tecnologia::Outras Engenharias e TecnologiasScience & TechnologyEngineering complex tissues requires the use of advanced biofabrication techniques that allow the replication of the tissue's 3D microenvironment, architecture and cellular interactions. In the case of skin, the most successful strategies to introduce the complexity of hair follicle (HF) appendages have highlighted the importance of facilitating direct interaction between dermal papilla (DP) cells and keratinocytes (KCs) in organotypic skin models. In this work, we took advantage of microscopy-guided laser ablation (MGLA) to microfabricate a fibroblast-populated collagen hydrogel and create a subcompartment that guides the migration of KCs and lead their interaction with DP cells to recreate follicular structures. Upon definition of the processing parameters (laser incidence area and power), MGLA was used to create 3D microchannels from the surface of a standard organotypic human skin model up to the aggregates containing DP cells and KCs, previously incorporated into the dermal-like fibroblast-collagen layer. Analysis of the constructs showed that the fabricated microfeatures successfully guided the fusion between epidermal and aggregates keratinocytes, which differentiated into follicular-like structures within the organotypic human skin model, increasing its functionality. In summary, we demonstrate the fabrication of a highly structured 3D hydrogel-based construct using MGLA to attain a complex skin model bearing folliculoid structures, highlighting its potential use as an in vitro platform to study the mechanisms controlling HF development or for the screening of bioactive substances.The authors would like to acknowledge the financial support from the Consolidator Grant “ECM_INK” (ERC-2016-COG-726061) and from FCT/MCTES (Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia, e Ensino Superior) grants PD/59/2013, PD/BD/113800/2015 (Carla Abreu), PD/169/2013 (Manuela E. L. Lago) and IF/00945/2014 (Alexandra P. Marques). The authors also acknowledge the financial support from the FSE/POCH (Fundo Social Europeu através do Programa Operacional do Capital Humano) under the scope of the NORTE-08-5369-FSE-000037 (Manuela E. L. Lago) grant.John Wiley & Sons LtdUniversidade do MinhoAbreu, C. M.Gasperini, LucaLago, Manuela Ermelinda LopesReis, R. L.Marques, A. P.2021-102021-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75131engAbreu C. M., Gasperini L., Lago M. E. L., Reis R. L., Marques A. P. Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages, Bioengineering and Translational Medicine, pp. e10195, doi:10.1002/btm2.10195, 20212380-676110.1002/btm2.10195e10195https://aiche.onlinelibrary.wiley.com/doi/full/10.1002/btm2.10195info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:17Zoai:repositorium.sdum.uminho.pt:1822/75131Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:12.198329Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages |
title |
Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages |
spellingShingle |
Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages Abreu, C. M. hair follicle In Vitro Model Laser ablation 3D tissue model biofabrication dermal papilla skin model Engenharia e Tecnologia::Outras Engenharias e Tecnologias Science & Technology |
title_short |
Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages |
title_full |
Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages |
title_fullStr |
Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages |
title_full_unstemmed |
Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages |
title_sort |
Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages |
author |
Abreu, C. M. |
author_facet |
Abreu, C. M. Gasperini, Luca Lago, Manuela Ermelinda Lopes Reis, R. L. Marques, A. P. |
author_role |
author |
author2 |
Gasperini, Luca Lago, Manuela Ermelinda Lopes Reis, R. L. Marques, A. P. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Abreu, C. M. Gasperini, Luca Lago, Manuela Ermelinda Lopes Reis, R. L. Marques, A. P. |
dc.subject.por.fl_str_mv |
hair follicle In Vitro Model Laser ablation 3D tissue model biofabrication dermal papilla skin model Engenharia e Tecnologia::Outras Engenharias e Tecnologias Science & Technology |
topic |
hair follicle In Vitro Model Laser ablation 3D tissue model biofabrication dermal papilla skin model Engenharia e Tecnologia::Outras Engenharias e Tecnologias Science & Technology |
description |
Engineering complex tissues requires the use of advanced biofabrication techniques that allow the replication of the tissue's 3D microenvironment, architecture and cellular interactions. In the case of skin, the most successful strategies to introduce the complexity of hair follicle (HF) appendages have highlighted the importance of facilitating direct interaction between dermal papilla (DP) cells and keratinocytes (KCs) in organotypic skin models. In this work, we took advantage of microscopy-guided laser ablation (MGLA) to microfabricate a fibroblast-populated collagen hydrogel and create a subcompartment that guides the migration of KCs and lead their interaction with DP cells to recreate follicular structures. Upon definition of the processing parameters (laser incidence area and power), MGLA was used to create 3D microchannels from the surface of a standard organotypic human skin model up to the aggregates containing DP cells and KCs, previously incorporated into the dermal-like fibroblast-collagen layer. Analysis of the constructs showed that the fabricated microfeatures successfully guided the fusion between epidermal and aggregates keratinocytes, which differentiated into follicular-like structures within the organotypic human skin model, increasing its functionality. In summary, we demonstrate the fabrication of a highly structured 3D hydrogel-based construct using MGLA to attain a complex skin model bearing folliculoid structures, highlighting its potential use as an in vitro platform to study the mechanisms controlling HF development or for the screening of bioactive substances. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10 2021-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/75131 |
url |
http://hdl.handle.net/1822/75131 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Abreu C. M., Gasperini L., Lago M. E. L., Reis R. L., Marques A. P. Microscopy-guided laser ablation for the creation of complex skin models with folliculoid appendages, Bioengineering and Translational Medicine, pp. e10195, doi:10.1002/btm2.10195, 2021 2380-6761 10.1002/btm2.10195 e10195 https://aiche.onlinelibrary.wiley.com/doi/full/10.1002/btm2.10195 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley & Sons Ltd |
publisher.none.fl_str_mv |
John Wiley & Sons Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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