Molecular studies on HSV: replication rate, infection capacity and progeny

Detalhes bibliográficos
Autor(a) principal: Azevedo, Aleksandra
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8320
Resumo: Dissertação de mestrado em Genética molecular e Biomedicina, apresentada à Faculdade de Ciências e Tecnologias da Universidade Nova de Lisboa, 2016.
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spelling Molecular studies on HSV: replication rate, infection capacity and progenySexually Transmitted DiseasesHSV1HSV2Herpes Simplex VirusReplication RateInfection CapacityProgenyCell CultureInfecções Sexualmente TransmissíveisHerpes GenitalCapacidade de InfeçãoProgeniaCultura CelularDissertação de mestrado em Genética molecular e Biomedicina, apresentada à Faculdade de Ciências e Tecnologias da Universidade Nova de Lisboa, 2016.Herpes simplex viruses (HSV) are ubiquitous host-adapted pathogens that cause a variety of different disorders. There are two sub-types: HSV-1, which is traditionally associated with oro-facial infections, and HSV-2 that is mostly associated with genital ulcers. This distinction, however, is becoming less evident since HSV-1 frequency in genital infections is increasing due to social, demographic and migratory tendencies, making genital herpes one of the most prevalent sexually transmitted infections worldwide. A better understanding on genital HSV-1 and HSV-2 infections is mandatory to the pathogenesis of human herpes disease. The scope of this thesis was to evaluate the life cycle of various HSV-1 and HSV-2 genital clinical isolates with different viral loads in three distinct host cell lines, giving special focus on both capacity and efficiency of viral infection, in terms of replication rate and progeny. Our results showed that: i) both HSV-1 and HSV-2 isolates exhibited similar infection patterns regardless MOI, with DNA starting to be synthesized nearly at 6-12h post-infection; ii) regardless HSV subtype, initial viral concentrations do not apparently affect adherence to any host cell line nor the generated progeny; iii) Vero E6 cells seemed the most appropriated cell line for HSV-2 infection; iv) HeLa229 cells appeared to be the most suitable for HSV-1 infection for smaller inoculums; and v) Vero cell line had the worst viral growth results for both HSV subtypes. In general, HSV-2 displayed always lower both attachment capacities and growth rates than HSV-1, although higher progenies were seen in Vero E6 cell line. Overall, the findings presented in this MSc thesis will certainly constitute a step forward for the understanding of the pathogenesis of the human herpes genital infections.Herpes simplex viruses (HSV) are ubiquitous host-adapted pathogens that cause a variety of different disorders. There are two sub-types: HSV-1, which is traditionally associated with oro-facial infections, and HSV-2 that is mostly associated with genital ulcers. This distinction, however, is becoming less evident since HSV-1 frequency in genital infections is increasing due to social, demographic and migratory tendencies, making genital herpes one of the most prevalent sexually transmitted infections worldwide. A better understanding on genital HSV-1 and HSV-2 infections is mandatory to the pathogenesis of human herpes disease. The scope of this thesis was to evaluate the life cycle of various HSV-1 and HSV-2 genital clinical isolates with different viral loads in three distinct host cell lines, giving special focus on both capacity and efficiency of viral infection, in terms of replication rate and progeny. Our results showed that: i) both HSV-1 and HSV-2 isolates exhibited similar infection patterns regardless MOI, with DNA starting to be synthesized nearly at 6-12h post-infection; ii) regardless HSV subtype, initial viral concentrations do not apparently affect adherence to any host cell line nor the generated progeny; iii) Vero E6 cells seemed the most appropriated cell line for HSV-2 infection; iv) HeLa229 cells appeared to be the most suitable for HSV-1 infection for smaller inoculums; and v) Vero cell line had the worst viral growth results for both HSV subtypes. In general, HSV-2 displayed always lower both attachment capacities and growth rates than HSV-1, although higher progenies were seen in Vero E6 cell line. Overall, the findings presented in this MSc thesis will certainly constitute a step forward for the understanding of the pathogenesis of the human herpes genital infections.Lopo, SílviaNunes, AlexandraRepositório Científico do Instituto Nacional de SaúdeAzevedo, Aleksandra2022-11-18T14:35:51Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.18/8320enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:27Zoai:repositorio.insa.pt:10400.18/8320Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:39:26.089950Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Molecular studies on HSV: replication rate, infection capacity and progeny
title Molecular studies on HSV: replication rate, infection capacity and progeny
spellingShingle Molecular studies on HSV: replication rate, infection capacity and progeny
Azevedo, Aleksandra
Sexually Transmitted Diseases
HSV1
HSV2
Herpes Simplex Virus
Replication Rate
Infection Capacity
Progeny
Cell Culture
Infecções Sexualmente Transmissíveis
Herpes Genital
Capacidade de Infeção
Progenia
Cultura Celular
title_short Molecular studies on HSV: replication rate, infection capacity and progeny
title_full Molecular studies on HSV: replication rate, infection capacity and progeny
title_fullStr Molecular studies on HSV: replication rate, infection capacity and progeny
title_full_unstemmed Molecular studies on HSV: replication rate, infection capacity and progeny
title_sort Molecular studies on HSV: replication rate, infection capacity and progeny
author Azevedo, Aleksandra
author_facet Azevedo, Aleksandra
author_role author
dc.contributor.none.fl_str_mv Lopo, Sílvia
Nunes, Alexandra
Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Azevedo, Aleksandra
dc.subject.por.fl_str_mv Sexually Transmitted Diseases
HSV1
HSV2
Herpes Simplex Virus
Replication Rate
Infection Capacity
Progeny
Cell Culture
Infecções Sexualmente Transmissíveis
Herpes Genital
Capacidade de Infeção
Progenia
Cultura Celular
topic Sexually Transmitted Diseases
HSV1
HSV2
Herpes Simplex Virus
Replication Rate
Infection Capacity
Progeny
Cell Culture
Infecções Sexualmente Transmissíveis
Herpes Genital
Capacidade de Infeção
Progenia
Cultura Celular
description Dissertação de mestrado em Genética molecular e Biomedicina, apresentada à Faculdade de Ciências e Tecnologias da Universidade Nova de Lisboa, 2016.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
2022-11-18T14:35:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8320
url http://hdl.handle.net/10400.18/8320
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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