Human biomonitoring : biomarkers, individual susceptibility, and nutrigenetics

Detalhes bibliográficos
Autor(a) principal: Ladeira, Carina
Data de Publicação: 2014
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/15963
Resumo: Tese de doutoramento, Biologia (Genética), Universidade de Lisboa, Faculdade de Ciências, 2015
id RCAP_91edc5b7c69d5cadbab4286355c6220d
oai_identifier_str oai:repositorio.ul.pt:10451/15963
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Human biomonitoring : biomarkers, individual susceptibility, and nutrigeneticsEpidemiologia molecularBiomarcadoresGenotoxicidadeMovimento celularNutrigenómicaTeses de doutoramento - 2015Tese de doutoramento, Biologia (Genética), Universidade de Lisboa, Faculdade de Ciências, 2015Cytokinesis blocked micronucleus (CBMN) assay and comet assay are the most promising short-term genotoxicity assays for human risk assessment and their combination is recommended to monitor populations chronically exposed to genotoxic agents. Nutrition is recognized to be an important lifestyle factor that influences cancer risk, and should be taken into account at an individual level. Laboratories are occupational settings where chemical agents are handled and workers are exposed. Formaldehyde and cytostatic drugs, in particular, are chemical agents handled in laboratories that are considered carcinogenic for humans and special protective measures should thus be adopted against them. The aim of this investigation is to contribute to the development of a biomonitoring programme that includes genotoxicity assessment related with genetic susceptibility biomarkers, and lifestyle factors, namely nutrition. The experimental planning used was a case-control blinded study. Four separated samples were formed comprising two samples of subjects exposed (n = 56 for formaldehyde; n = 46 for cytostatics), and two samples of non-exposed controls (n = 85 and n = 46, respectively). Participants filled-in a personal and a food frequency questionnaires. CBMN and comet assays were used to assess genotoxicity. Individual susceptibility was investigated by Real Time PCR. Measurements of serum vitamins A and E were performed by HPLC, and vitamin D by ELISA. The risk of genotoxicity in those exposed was then compared with the risk in the controls, allowing for the quantitative measurement of association between exposure and genotoxicity. In both occupational settings, the genotoxicity biomarkers were significantly higher in the exposed than in the non-exposed controls (p<0.05). In the formaldehyde occupational context, significant associations were found between XRCC3 genotypes and nuclear buds; but that was not the case either for ADH5 or VDR. Comet assay did not identify significant differences between those exposed to cytostatics and controls, and the same lack of association applies to the OGG1 genotypes. Micronutrients association to the genotoxicity biomarkers was controversial; we have found positive correlations for vitamin A and negative ones for vitamin E. Our findings emphasize the need for the implementation of a regular biomonitoring programme of personnel occupationally exposed to drugs like formaldehyde and cytostatics.Fundação para a Ciência e a Tecnologia, IP (FCT)Gomes, M. Freitas, 1949-Brito, Rui Miguel Duque de, 1969-Repositório da Universidade de LisboaLadeira, Carina2015-02-09T11:47:33Z201520142015-01-01T00:00:00Zdoctoral thesisinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10451/15963TID:101324944enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:21:44Zoai:repositorio.ul.pt:10451/15963Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:21:44Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Human biomonitoring : biomarkers, individual susceptibility, and nutrigenetics
title Human biomonitoring : biomarkers, individual susceptibility, and nutrigenetics
spellingShingle Human biomonitoring : biomarkers, individual susceptibility, and nutrigenetics
Ladeira, Carina
Epidemiologia molecular
Biomarcadores
Genotoxicidade
Movimento celular
Nutrigenómica
Teses de doutoramento - 2015
title_short Human biomonitoring : biomarkers, individual susceptibility, and nutrigenetics
title_full Human biomonitoring : biomarkers, individual susceptibility, and nutrigenetics
title_fullStr Human biomonitoring : biomarkers, individual susceptibility, and nutrigenetics
title_full_unstemmed Human biomonitoring : biomarkers, individual susceptibility, and nutrigenetics
title_sort Human biomonitoring : biomarkers, individual susceptibility, and nutrigenetics
author Ladeira, Carina
author_facet Ladeira, Carina
author_role author
dc.contributor.none.fl_str_mv Gomes, M. Freitas, 1949-
Brito, Rui Miguel Duque de, 1969-
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Ladeira, Carina
dc.subject.por.fl_str_mv Epidemiologia molecular
Biomarcadores
Genotoxicidade
Movimento celular
Nutrigenómica
Teses de doutoramento - 2015
topic Epidemiologia molecular
Biomarcadores
Genotoxicidade
Movimento celular
Nutrigenómica
Teses de doutoramento - 2015
description Tese de doutoramento, Biologia (Genética), Universidade de Lisboa, Faculdade de Ciências, 2015
publishDate 2014
dc.date.none.fl_str_mv 2014
2015-02-09T11:47:33Z
2015
2015-01-01T00:00:00Z
dc.type.driver.fl_str_mv doctoral thesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/15963
TID:101324944
url http://hdl.handle.net/10451/15963
identifier_str_mv TID:101324944
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817548841071673344

Registros relacionados