Genetic Determinants of High-Level Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureus

Detalhes bibliográficos
Autor(a) principal: Pardos de la Gandara, Maria
Data de Publicação: 2018
Outros Autores: Borges, Vitor, Chung, Marilyn, Milheiriço, Catarina, Gomes, João Paulo, de Lencastre, Herminia, Tomasz, Alexander
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/6281
Resumo: Free PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5971597/
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spelling Genetic Determinants of High-Level Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureusStaphylococcus aureusOxacillinMRSAOxacillin Resistance DeterminantsGuanine Metabolism;Free PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5971597/Erratum in: Correction for Pardos de la Gandara et al., "Genetic Determinants of High-Level Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureus". Antimicrob Agents Chemother. 2018 Jun 26;62(7). pii: e01096-18. doi: 10.1128/AAC.01096-18. Print 2018 Jul. Disponível em: https://aac.asm.org/content/62/7/e01096-18.longMethicillin-resistant Staphylococcus aureus (MRSA) strains carry either a mecA- or a mecC-mediated mechanism of resistance to beta-lactam antibiotics, and the phenotypic expression of resistance shows extensive strain-to-strain variation. In recent communications, we identified the genetic determinants associated with the stringent stress response that play a major role in the antibiotic resistant phenotype of the historically earliest "archaic" clone of MRSA and in the mecC-carrying MRSA strain LGA251. Here, we sought to test whether or not the same genetic determinants also contribute to the resistant phenotype of highly and homogeneously resistant (H*R) derivatives of a major contemporary MRSA clone, USA300. We found that the resistance phenotype was linked to six genes (fruB, gmk, hpt, purB, prsA, and relA), which were most frequently targeted among the analyzed 20 H*R strains (one mutation per clone in 19 of the 20 H*R strains). Besides the strong parallels with our previous findings (five of the six genes matched), all but one of the repeatedly targeted genes were found to be linked to guanine metabolism, pointing to the key role that this pathway plays in defining the level of antibiotic resistance independent of the clonal type of MRSA.This work was financially supported by a US Public Health Service Award 2 R01 AI457838-15 and by project LISBOA-01-0145-FEDER-007660 (Microbiologia Molecular, Estrutural e Celular) funded by FEDER funds through COMPETE2020-Programa Operacional Competitividade e Internacionalização (POCI), by national funds through FCTFundação para a Ciência e a Tecnologia and project ONEIDA (LISBOA-01-0145-FEDER016417) cofunded by FEEI-“Fundos Europeus Estruturais e de Investimento” from “Programa Operacional Regional Lisboa 2020,” and by national funds from FCT. M.P.D.L.G. was supported by PCORI grant CER-1402-10800 and by funds from the RB Roberts Bacterial Antibiotic Resistance Group (BARG). C.M. was supported by grant SFRH/BPD/111697/2015 from FCT.American Society for MicrobiologyRepositório Científico do Instituto Nacional de SaúdePardos de la Gandara, MariaBorges, VitorChung, MarilynMilheiriço, CatarinaGomes, João Paulode Lencastre, HerminiaTomasz, Alexander2019-03-22T16:13:22Z2018-05-252018-05-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.18/6281engAntimicrob Agents Chemother. 2018 May 25;62(6):pii=e00206-18. doi: 10.1128/AAC.00206-18. Print 2018 Jun0066-480410.1128/AAC.00206-18info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:19Zoai:repositorio.insa.pt:10400.18/6281Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:40:56.562689Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genetic Determinants of High-Level Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureus
title Genetic Determinants of High-Level Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureus
spellingShingle Genetic Determinants of High-Level Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureus
Pardos de la Gandara, Maria
Staphylococcus aureus
Oxacillin
MRSA
Oxacillin Resistance Determinants
Guanine Metabolism;
title_short Genetic Determinants of High-Level Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureus
title_full Genetic Determinants of High-Level Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureus
title_fullStr Genetic Determinants of High-Level Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureus
title_full_unstemmed Genetic Determinants of High-Level Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureus
title_sort Genetic Determinants of High-Level Oxacillin Resistance in Methicillin-Resistant Staphylococcus aureus
author Pardos de la Gandara, Maria
author_facet Pardos de la Gandara, Maria
Borges, Vitor
Chung, Marilyn
Milheiriço, Catarina
Gomes, João Paulo
de Lencastre, Herminia
Tomasz, Alexander
author_role author
author2 Borges, Vitor
Chung, Marilyn
Milheiriço, Catarina
Gomes, João Paulo
de Lencastre, Herminia
Tomasz, Alexander
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Pardos de la Gandara, Maria
Borges, Vitor
Chung, Marilyn
Milheiriço, Catarina
Gomes, João Paulo
de Lencastre, Herminia
Tomasz, Alexander
dc.subject.por.fl_str_mv Staphylococcus aureus
Oxacillin
MRSA
Oxacillin Resistance Determinants
Guanine Metabolism;
topic Staphylococcus aureus
Oxacillin
MRSA
Oxacillin Resistance Determinants
Guanine Metabolism;
description Free PMC Article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5971597/
publishDate 2018
dc.date.none.fl_str_mv 2018-05-25
2018-05-25T00:00:00Z
2019-03-22T16:13:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/6281
url http://hdl.handle.net/10400.18/6281
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Antimicrob Agents Chemother. 2018 May 25;62(6):pii=e00206-18. doi: 10.1128/AAC.00206-18. Print 2018 Jun
0066-4804
10.1128/AAC.00206-18
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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