Understanding the anti-obesity potential of an avocado oil-rich cheese through an in vitro co-culture intestine cell model

Detalhes bibliográficos
Autor(a) principal: Machado, Manuela
Data de Publicação: 2023
Outros Autores: Costa, Eduardo M., Silva, Sara, Rodriguez-Alcalá, Luís M., Gomes, Ana Maria, Pintado, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/42391
Resumo: Nowadays, with consumers’ requirements shifting towards more natural solutions and the advent of nutraceutical-based approaches, new alternatives for obesity management are being developed. This work aimed to show, for the first time, the potential of avocado oil-fortified cheese as a viable foodstuff for obesity management through complex in vitro cellular models. The results showed that oleic and palmitic acids’ permeability through the Caco-2/HT29-MTX membrane peaked at the 2h mark, with the highest apparent permeability being registered for oleic acid (0.14 cm/s). Additionally, the permeated compounds were capable of modulating the metabolism of adipocytes present in the basal compartment, significantly reducing adipokine (leptin) and cytokine (MPC-1, IL-10, and TNF-α) production. The permeates (containing 3.30 µg/mL of palmitic acid and 2.16 µg/mL of oleic acid) also presented an overall anti-inflammatory activity upon Raw 264.7 macrophages, reducing IL-6 and TNF-α secretion. Despite in vivo assays being required, the data showed the potential of a functional dairy product as a valid food matrix to aid in obesity management.
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spelling Understanding the anti-obesity potential of an avocado oil-rich cheese through an in vitro co-culture intestine cell modelAnti-inflammatory potentialCell-based assaysLipid digestionPermeabilityNowadays, with consumers’ requirements shifting towards more natural solutions and the advent of nutraceutical-based approaches, new alternatives for obesity management are being developed. This work aimed to show, for the first time, the potential of avocado oil-fortified cheese as a viable foodstuff for obesity management through complex in vitro cellular models. The results showed that oleic and palmitic acids’ permeability through the Caco-2/HT29-MTX membrane peaked at the 2h mark, with the highest apparent permeability being registered for oleic acid (0.14 cm/s). Additionally, the permeated compounds were capable of modulating the metabolism of adipocytes present in the basal compartment, significantly reducing adipokine (leptin) and cytokine (MPC-1, IL-10, and TNF-α) production. The permeates (containing 3.30 µg/mL of palmitic acid and 2.16 µg/mL of oleic acid) also presented an overall anti-inflammatory activity upon Raw 264.7 macrophages, reducing IL-6 and TNF-α secretion. Despite in vivo assays being required, the data showed the potential of a functional dairy product as a valid food matrix to aid in obesity management.Veritati - Repositório Institucional da Universidade Católica PortuguesaMachado, ManuelaCosta, Eduardo M.Silva, SaraRodriguez-Alcalá, Luís M.Gomes, Ana MariaPintado, Manuela2023-09-15T14:35:18Z2023-08-072023-08-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/42391eng1420-304910.3390/molecules2815592385167767472PMC1042117637570893001046310500001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-19T01:42:31Zoai:repositorio.ucp.pt:10400.14/42391Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:29:35.997269Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Understanding the anti-obesity potential of an avocado oil-rich cheese through an in vitro co-culture intestine cell model
title Understanding the anti-obesity potential of an avocado oil-rich cheese through an in vitro co-culture intestine cell model
spellingShingle Understanding the anti-obesity potential of an avocado oil-rich cheese through an in vitro co-culture intestine cell model
Machado, Manuela
Anti-inflammatory potential
Cell-based assays
Lipid digestion
Permeability
title_short Understanding the anti-obesity potential of an avocado oil-rich cheese through an in vitro co-culture intestine cell model
title_full Understanding the anti-obesity potential of an avocado oil-rich cheese through an in vitro co-culture intestine cell model
title_fullStr Understanding the anti-obesity potential of an avocado oil-rich cheese through an in vitro co-culture intestine cell model
title_full_unstemmed Understanding the anti-obesity potential of an avocado oil-rich cheese through an in vitro co-culture intestine cell model
title_sort Understanding the anti-obesity potential of an avocado oil-rich cheese through an in vitro co-culture intestine cell model
author Machado, Manuela
author_facet Machado, Manuela
Costa, Eduardo M.
Silva, Sara
Rodriguez-Alcalá, Luís M.
Gomes, Ana Maria
Pintado, Manuela
author_role author
author2 Costa, Eduardo M.
Silva, Sara
Rodriguez-Alcalá, Luís M.
Gomes, Ana Maria
Pintado, Manuela
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Machado, Manuela
Costa, Eduardo M.
Silva, Sara
Rodriguez-Alcalá, Luís M.
Gomes, Ana Maria
Pintado, Manuela
dc.subject.por.fl_str_mv Anti-inflammatory potential
Cell-based assays
Lipid digestion
Permeability
topic Anti-inflammatory potential
Cell-based assays
Lipid digestion
Permeability
description Nowadays, with consumers’ requirements shifting towards more natural solutions and the advent of nutraceutical-based approaches, new alternatives for obesity management are being developed. This work aimed to show, for the first time, the potential of avocado oil-fortified cheese as a viable foodstuff for obesity management through complex in vitro cellular models. The results showed that oleic and palmitic acids’ permeability through the Caco-2/HT29-MTX membrane peaked at the 2h mark, with the highest apparent permeability being registered for oleic acid (0.14 cm/s). Additionally, the permeated compounds were capable of modulating the metabolism of adipocytes present in the basal compartment, significantly reducing adipokine (leptin) and cytokine (MPC-1, IL-10, and TNF-α) production. The permeates (containing 3.30 µg/mL of palmitic acid and 2.16 µg/mL of oleic acid) also presented an overall anti-inflammatory activity upon Raw 264.7 macrophages, reducing IL-6 and TNF-α secretion. Despite in vivo assays being required, the data showed the potential of a functional dairy product as a valid food matrix to aid in obesity management.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-15T14:35:18Z
2023-08-07
2023-08-07T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/42391
url http://hdl.handle.net/10400.14/42391
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1420-3049
10.3390/molecules28155923
85167767472
PMC10421176
37570893
001046310500001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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