In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differences
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/3342 |
Resumo: | Identification of differences between genetically modified plants and their original counterparts plays a central role in risk assessment strategy. Our main goal was to better understand the relevance of transgene presence, genetic, and epigenetic changes induced by transgene insertion, and in vitro culture in putative unintended differences between a transgenic and its comparator. Thus, we have used multiplex fluorescence 2DE coupled with MS to characterize the proteome of three different rice lines (Oryza sativa L. ssp. japonica cv. Nipponbare): a control conventional line (C), an Agrobacterium-transformed transgenic line (Ta) and a negative segregant (NSb). We observed that Ta and NSb appeared identical (with only one spot differentially abundant--fold difference ≥ 1.5), contrasting with the control (49 spots with fold difference ≥ 1.5, in both Ta and NSb vs. control). Given that in vitro culture was the only event in common between Ta and NSb, we hypothesize that in vitro culture stress was the most relevant condition contributing for the observed proteomic differences. MS protein identification support our hypothesis, indicating that Ta and NSb lines adjusted their metabolic pathways and altered the abundance of several stress related proteins in order to cope with in vitro culture. |
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In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differencesSegurança AlimentarIn vitro culturePlant proteomicsIdentification of differences between genetically modified plants and their original counterparts plays a central role in risk assessment strategy. Our main goal was to better understand the relevance of transgene presence, genetic, and epigenetic changes induced by transgene insertion, and in vitro culture in putative unintended differences between a transgenic and its comparator. Thus, we have used multiplex fluorescence 2DE coupled with MS to characterize the proteome of three different rice lines (Oryza sativa L. ssp. japonica cv. Nipponbare): a control conventional line (C), an Agrobacterium-transformed transgenic line (Ta) and a negative segregant (NSb). We observed that Ta and NSb appeared identical (with only one spot differentially abundant--fold difference ≥ 1.5), contrasting with the control (49 spots with fold difference ≥ 1.5, in both Ta and NSb vs. control). Given that in vitro culture was the only event in common between Ta and NSb, we hypothesize that in vitro culture stress was the most relevant condition contributing for the observed proteomic differences. MS protein identification support our hypothesis, indicating that Ta and NSb lines adjusted their metabolic pathways and altered the abundance of several stress related proteins in order to cope with in vitro culture.ProteomicsRepositório Científico do Instituto Nacional de SaúdeFonseca, C.Planchon, S.Serra, T.Chander, S.Saibo, N.J.M.Renaut, J.Oliveira, M.M.Batista, Rita2016-02-16T16:33:25Z2015-012015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3342engProteomics. 2015 Jan;15(1):124-34. doi: 10.1002/pmic.201400018. Epub 2014 Dec 41615-985310.1002/pmic.201400018metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:34Zoai:repositorio.insa.pt:10400.18/3342Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:37:58.970121Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differences |
title |
In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differences |
spellingShingle |
In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differences Fonseca, C. Segurança Alimentar In vitro culture Plant proteomics |
title_short |
In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differences |
title_full |
In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differences |
title_fullStr |
In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differences |
title_full_unstemmed |
In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differences |
title_sort |
In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differences |
author |
Fonseca, C. |
author_facet |
Fonseca, C. Planchon, S. Serra, T. Chander, S. Saibo, N.J.M. Renaut, J. Oliveira, M.M. Batista, Rita |
author_role |
author |
author2 |
Planchon, S. Serra, T. Chander, S. Saibo, N.J.M. Renaut, J. Oliveira, M.M. Batista, Rita |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Fonseca, C. Planchon, S. Serra, T. Chander, S. Saibo, N.J.M. Renaut, J. Oliveira, M.M. Batista, Rita |
dc.subject.por.fl_str_mv |
Segurança Alimentar In vitro culture Plant proteomics |
topic |
Segurança Alimentar In vitro culture Plant proteomics |
description |
Identification of differences between genetically modified plants and their original counterparts plays a central role in risk assessment strategy. Our main goal was to better understand the relevance of transgene presence, genetic, and epigenetic changes induced by transgene insertion, and in vitro culture in putative unintended differences between a transgenic and its comparator. Thus, we have used multiplex fluorescence 2DE coupled with MS to characterize the proteome of three different rice lines (Oryza sativa L. ssp. japonica cv. Nipponbare): a control conventional line (C), an Agrobacterium-transformed transgenic line (Ta) and a negative segregant (NSb). We observed that Ta and NSb appeared identical (with only one spot differentially abundant--fold difference ≥ 1.5), contrasting with the control (49 spots with fold difference ≥ 1.5, in both Ta and NSb vs. control). Given that in vitro culture was the only event in common between Ta and NSb, we hypothesize that in vitro culture stress was the most relevant condition contributing for the observed proteomic differences. MS protein identification support our hypothesis, indicating that Ta and NSb lines adjusted their metabolic pathways and altered the abundance of several stress related proteins in order to cope with in vitro culture. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01 2015-01-01T00:00:00Z 2016-02-16T16:33:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/3342 |
url |
http://hdl.handle.net/10400.18/3342 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Proteomics. 2015 Jan;15(1):124-34. doi: 10.1002/pmic.201400018. Epub 2014 Dec 4 1615-9853 10.1002/pmic.201400018 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Proteomics |
publisher.none.fl_str_mv |
Proteomics |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132115787317248 |