New water-soluble amine-reactive reagents for labeling microbial cells: application to mural paintings
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/9333 |
Resumo: | Mural paintings are continuously exposed to physical, chemical and biological degradation, however, among the biological agents that cause deterioration, microorganisms are of critical importance, due to their metabolic versatility and flexibility, and their ability to tolerate unfavourable conditions. Thereby, microorganisms can cause damages in mural paintings surfaces through a variety of mechanisms, including biofilm formation, chemical reactions, physical penetration into the substrate and production of pigments [1-3]. It is imperative the development of new strategies that detect microbial contamination in order to plan effective remediation strategies. This research focuses on the application of fluorescent compounds. In this context we developed new water-soluble coumarin derivatives (4-Sulfotetrafluorophenyl Esters) as long-wavelength fluorescent labels for biomolecules[4]. These esters are easily synthesized, and react readily with primary amines to give acylation products. |
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New water-soluble amine-reactive reagents for labeling microbial cells: application to mural paintingswater-soluble amine-reactive reagentslabelingmicrobial cellsmural paintingsMural paintings are continuously exposed to physical, chemical and biological degradation, however, among the biological agents that cause deterioration, microorganisms are of critical importance, due to their metabolic versatility and flexibility, and their ability to tolerate unfavourable conditions. Thereby, microorganisms can cause damages in mural paintings surfaces through a variety of mechanisms, including biofilm formation, chemical reactions, physical penetration into the substrate and production of pigments [1-3]. It is imperative the development of new strategies that detect microbial contamination in order to plan effective remediation strategies. This research focuses on the application of fluorescent compounds. In this context we developed new water-soluble coumarin derivatives (4-Sulfotetrafluorophenyl Esters) as long-wavelength fluorescent labels for biomolecules[4]. These esters are easily synthesized, and react readily with primary amines to give acylation products.University of Evora2014-01-07T19:00:52Z2014-01-072013-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/9333http://hdl.handle.net/10174/9333eng1st International Conference on Innovation in Art Research and Technology Hercules Laboratory – University of Evora ISBN: 978-606-624-441-1naonaosimQUIndndndamlp@uevora.pt307Martins, SérgioRosado, TâniaCaldeira, AnaPereira, Antónioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:51:20Zoai:dspace.uevora.pt:10174/9333Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:03:28.636877Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
New water-soluble amine-reactive reagents for labeling microbial cells: application to mural paintings |
title |
New water-soluble amine-reactive reagents for labeling microbial cells: application to mural paintings |
spellingShingle |
New water-soluble amine-reactive reagents for labeling microbial cells: application to mural paintings Martins, Sérgio water-soluble amine-reactive reagents labeling microbial cells mural paintings |
title_short |
New water-soluble amine-reactive reagents for labeling microbial cells: application to mural paintings |
title_full |
New water-soluble amine-reactive reagents for labeling microbial cells: application to mural paintings |
title_fullStr |
New water-soluble amine-reactive reagents for labeling microbial cells: application to mural paintings |
title_full_unstemmed |
New water-soluble amine-reactive reagents for labeling microbial cells: application to mural paintings |
title_sort |
New water-soluble amine-reactive reagents for labeling microbial cells: application to mural paintings |
author |
Martins, Sérgio |
author_facet |
Martins, Sérgio Rosado, Tânia Caldeira, Ana Pereira, António |
author_role |
author |
author2 |
Rosado, Tânia Caldeira, Ana Pereira, António |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Martins, Sérgio Rosado, Tânia Caldeira, Ana Pereira, António |
dc.subject.por.fl_str_mv |
water-soluble amine-reactive reagents labeling microbial cells mural paintings |
topic |
water-soluble amine-reactive reagents labeling microbial cells mural paintings |
description |
Mural paintings are continuously exposed to physical, chemical and biological degradation, however, among the biological agents that cause deterioration, microorganisms are of critical importance, due to their metabolic versatility and flexibility, and their ability to tolerate unfavourable conditions. Thereby, microorganisms can cause damages in mural paintings surfaces through a variety of mechanisms, including biofilm formation, chemical reactions, physical penetration into the substrate and production of pigments [1-3]. It is imperative the development of new strategies that detect microbial contamination in order to plan effective remediation strategies. This research focuses on the application of fluorescent compounds. In this context we developed new water-soluble coumarin derivatives (4-Sulfotetrafluorophenyl Esters) as long-wavelength fluorescent labels for biomolecules[4]. These esters are easily synthesized, and react readily with primary amines to give acylation products. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-01T00:00:00Z 2014-01-07T19:00:52Z 2014-01-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/9333 http://hdl.handle.net/10174/9333 |
url |
http://hdl.handle.net/10174/9333 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1st International Conference on Innovation in Art Research and Technology Hercules Laboratory – University of Evora ISBN: 978-606-624-441-1 nao nao sim QUI nd nd nd amlp@uevora.pt 307 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
University of Evora |
publisher.none.fl_str_mv |
University of Evora |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136518937247744 |