An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/10041 |
Resumo: | A nano-molecularly imprinted polymer (N-MIP) assembled on a screen-printed electrode for the cardiac troponin T (cTnT) was developed. The biomimetic surface was obtained by a co-polymer matrix as-sembled on the reduced graphene oxide (RGO) electrode surface. The cTnT active sites were engineered using pyrrole and carboxylated pyrrole that was one-step electropolymerized jointly with cTnT by cyclic voltammetry. The stepwise preparation of the biomimetic surface was characterized by cyclic and dif-ferential pulse voltammetries using the ferrocyanide/ferricyanide as redox probe. Structural and mor-phological characterization was also performed. The optimal relation of pyrrole and pyrrole-3-acid car-boxylic to perform the cTnT biomimetic nanosurface was obtained at 1:5 ratio. The analytical perfor-mance of cTnT N-MIP performed by differential pulse voltammetry showed a linear range from 0.01 to 0.1 ng mL-1 (r¼0.995, p«0.01), with a very low limit of detection (0.006 ng mL-1). The synergic effect of conductive polymer and graphene forming 3D structures of reactive sites resulted in a N-MIP with ex-cellent affinity to cTnT binding (KD¼7.3 10-13 mol L-1). The N-MIP proposed is based on a simple method of antibody obtaining with a large potential for point-of-care testing applications. |
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An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymerCardiac troponin TMolecularly imprinted polymerPyrroleAcid carboxylic-3-pyrroleGraphenePoint-of-care testingA nano-molecularly imprinted polymer (N-MIP) assembled on a screen-printed electrode for the cardiac troponin T (cTnT) was developed. The biomimetic surface was obtained by a co-polymer matrix as-sembled on the reduced graphene oxide (RGO) electrode surface. The cTnT active sites were engineered using pyrrole and carboxylated pyrrole that was one-step electropolymerized jointly with cTnT by cyclic voltammetry. The stepwise preparation of the biomimetic surface was characterized by cyclic and dif-ferential pulse voltammetries using the ferrocyanide/ferricyanide as redox probe. Structural and mor-phological characterization was also performed. The optimal relation of pyrrole and pyrrole-3-acid car-boxylic to perform the cTnT biomimetic nanosurface was obtained at 1:5 ratio. The analytical perfor-mance of cTnT N-MIP performed by differential pulse voltammetry showed a linear range from 0.01 to 0.1 ng mL-1 (r¼0.995, p«0.01), with a very low limit of detection (0.006 ng mL-1). The synergic effect of conductive polymer and graphene forming 3D structures of reactive sites resulted in a N-MIP with ex-cellent affinity to cTnT binding (KD¼7.3 10-13 mol L-1). The N-MIP proposed is based on a simple method of antibody obtaining with a large potential for point-of-care testing applications.ElsevierRepositório Científico do Instituto Politécnico do PortoSilva, Bárbara V.M.Rodríguez, Blanca A.G.Sales, GoretiSotomayor, Maria Del Pilar T.Dutra, Rosa F.20162117-01-01T00:00:00Z2016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/10041eng10.1016/j.bios.2015.10.068metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:51:32Zoai:recipp.ipp.pt:10400.22/10041Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:30:30.624908Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer |
title |
An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer |
spellingShingle |
An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer Silva, Bárbara V.M. Cardiac troponin T Molecularly imprinted polymer Pyrrole Acid carboxylic-3-pyrrole Graphene Point-of-care testing |
title_short |
An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer |
title_full |
An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer |
title_fullStr |
An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer |
title_full_unstemmed |
An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer |
title_sort |
An ultrasensitive human cardiac troponin T graphene screen-printed electrode based on electropolymerized-molecularly imprinted conducting polymer |
author |
Silva, Bárbara V.M. |
author_facet |
Silva, Bárbara V.M. Rodríguez, Blanca A.G. Sales, Goreti Sotomayor, Maria Del Pilar T. Dutra, Rosa F. |
author_role |
author |
author2 |
Rodríguez, Blanca A.G. Sales, Goreti Sotomayor, Maria Del Pilar T. Dutra, Rosa F. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Silva, Bárbara V.M. Rodríguez, Blanca A.G. Sales, Goreti Sotomayor, Maria Del Pilar T. Dutra, Rosa F. |
dc.subject.por.fl_str_mv |
Cardiac troponin T Molecularly imprinted polymer Pyrrole Acid carboxylic-3-pyrrole Graphene Point-of-care testing |
topic |
Cardiac troponin T Molecularly imprinted polymer Pyrrole Acid carboxylic-3-pyrrole Graphene Point-of-care testing |
description |
A nano-molecularly imprinted polymer (N-MIP) assembled on a screen-printed electrode for the cardiac troponin T (cTnT) was developed. The biomimetic surface was obtained by a co-polymer matrix as-sembled on the reduced graphene oxide (RGO) electrode surface. The cTnT active sites were engineered using pyrrole and carboxylated pyrrole that was one-step electropolymerized jointly with cTnT by cyclic voltammetry. The stepwise preparation of the biomimetic surface was characterized by cyclic and dif-ferential pulse voltammetries using the ferrocyanide/ferricyanide as redox probe. Structural and mor-phological characterization was also performed. The optimal relation of pyrrole and pyrrole-3-acid car-boxylic to perform the cTnT biomimetic nanosurface was obtained at 1:5 ratio. The analytical perfor-mance of cTnT N-MIP performed by differential pulse voltammetry showed a linear range from 0.01 to 0.1 ng mL-1 (r¼0.995, p«0.01), with a very low limit of detection (0.006 ng mL-1). The synergic effect of conductive polymer and graphene forming 3D structures of reactive sites resulted in a N-MIP with ex-cellent affinity to cTnT binding (KD¼7.3 10-13 mol L-1). The N-MIP proposed is based on a simple method of antibody obtaining with a large potential for point-of-care testing applications. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2117-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/10041 |
url |
http://hdl.handle.net/10400.22/10041 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bios.2015.10.068 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131400715108352 |