Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae)

Detalhes bibliográficos
Autor(a) principal: Nones, Stefano
Data de Publicação: 2021
Outros Autores: Simões, Fernanda, Trindade, Cândida Sofia, Matos, José, Sousa, Edmundo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51079
Resumo: The ambrosia beetle Platypus cylindrus is a major cork oak pest in Portugal. Beetles have different roles in host tree colonization and are equipped with specific structures (mycangia) for fungal transportation. The information on bacterial composition associated with mycangia is scarce. The bacterial community present in the mycangia of P. cylindrus male and female beetles collected from cork oak galleries was investigated. Mycangia anatomical structure was also explored using histological and 3D imaging techniques to highlight evidence of biological sexual dimorphism. A diverse bacterial community with few gender-specific bacteria was shown and histology revealed connections linking external and internal tissues only in females, providing the first insights into sexual differentiation for bacteria in a Platypodinae beetle species.
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spelling Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae)sexual dimorphismmetabarcodingPlatypodinaeprokaryotescork oakThe ambrosia beetle Platypus cylindrus is a major cork oak pest in Portugal. Beetles have different roles in host tree colonization and are equipped with specific structures (mycangia) for fungal transportation. The information on bacterial composition associated with mycangia is scarce. The bacterial community present in the mycangia of P. cylindrus male and female beetles collected from cork oak galleries was investigated. Mycangia anatomical structure was also explored using histological and 3D imaging techniques to highlight evidence of biological sexual dimorphism. A diverse bacterial community with few gender-specific bacteria was shown and histology revealed connections linking external and internal tissues only in females, providing the first insights into sexual differentiation for bacteria in a Platypodinae beetle species.The ambrosia beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae) is a major cork oak pest in Portugal. Female and male beetles have different roles in host tree colonization and are both equipped with prothoracic mycangia for fungal transportation. Despite a known beneficial role of bacteria in ambrosia beetles, information on bacterial composition associated with prothoracic mycangia structures is scarce. Bacterial community from mycangia of P. cylindrus male and female beetles collected from cork oak galleries was investigated by means of 16S metagenomics. Mycangia anatomical structure was also explored with histological techniques and X-ray computed microtomography to highlight evidence supporting biological sexual dimorphism. A bacterial community with highly diverse bacterial taxa with low abundances at the genus level was revealed. Lactobacillales, Leptotrichia, Neisseria, Rothia, and Sphingomonadaceae were significantly more abundant in males, while Acinetobacter, Chitinophagaceae, Enterobacteriaceae, Erwiniaceae, Microbacteriaceae, and Pseudoclavibacter were more abundant in females. Additionally, a core bacteriome of five genera was shared by both sexes. Histological examination revealed visible connections linking external and internal tissues in females, but none in males. Overall, these results provide the first insights into sexual differentiation for bacteria in a Platypodinae beetle species, identifying key patterns of bacteria distribution in the context of beetle ecology and functional behavior.MDPIRepositório da Universidade de LisboaNones, StefanoSimões, FernandaTrindade, Cândida SofiaMatos, JoséSousa, Edmundo2022-02-01T20:02:34Z2021-092021-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51079engNones, S.; Simões, F.; Trindade, C.S.; Matos, J.; Sousa, E. Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae). Insects 2021, 12, 881. https://doi.org/10.3390/ insects121008812075-445010.3390/insects12100881info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:55:34Zoai:repositorio.ul.pt:10451/51079Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:02:24.256248Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae)
title Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae)
spellingShingle Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae)
Nones, Stefano
sexual dimorphism
metabarcoding
Platypodinae
prokaryotes
cork oak
title_short Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae)
title_full Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae)
title_fullStr Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae)
title_full_unstemmed Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae)
title_sort Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae)
author Nones, Stefano
author_facet Nones, Stefano
Simões, Fernanda
Trindade, Cândida Sofia
Matos, José
Sousa, Edmundo
author_role author
author2 Simões, Fernanda
Trindade, Cândida Sofia
Matos, José
Sousa, Edmundo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Nones, Stefano
Simões, Fernanda
Trindade, Cândida Sofia
Matos, José
Sousa, Edmundo
dc.subject.por.fl_str_mv sexual dimorphism
metabarcoding
Platypodinae
prokaryotes
cork oak
topic sexual dimorphism
metabarcoding
Platypodinae
prokaryotes
cork oak
description The ambrosia beetle Platypus cylindrus is a major cork oak pest in Portugal. Beetles have different roles in host tree colonization and are equipped with specific structures (mycangia) for fungal transportation. The information on bacterial composition associated with mycangia is scarce. The bacterial community present in the mycangia of P. cylindrus male and female beetles collected from cork oak galleries was investigated. Mycangia anatomical structure was also explored using histological and 3D imaging techniques to highlight evidence of biological sexual dimorphism. A diverse bacterial community with few gender-specific bacteria was shown and histology revealed connections linking external and internal tissues only in females, providing the first insights into sexual differentiation for bacteria in a Platypodinae beetle species.
publishDate 2021
dc.date.none.fl_str_mv 2021-09
2021-09-01T00:00:00Z
2022-02-01T20:02:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51079
url http://hdl.handle.net/10451/51079
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nones, S.; Simões, F.; Trindade, C.S.; Matos, J.; Sousa, E. Microbiome Associated with the Mycangia of Female and Male Adults of the Ambrosia Beetle Platypus cylindrus Fab. (Coleoptera: Curculionidae). Insects 2021, 12, 881. https://doi.org/10.3390/ insects12100881
2075-4450
10.3390/insects12100881
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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