Membrane tension regulates water transport in yeast
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/5802 |
Resumo: | Evidence that membrane surface tension regulates water fluxes in intact cells of a Saccharomyces cerevisiae strain overexpressing aquaporin AQY1 was obtained by assessing the osmotic water transport parameters in cells equilibrated in different osmolarities. The osmotic water permeability coefficients (Pf) obtained for yeast cells overexpressing AQY1 incubated in low osmolarity buffers were similar to those obtained for a double mutant aqy1aqy2 and approximately three times lower (with higher activation energy, Ea) than values obtained for cells incubated in higher osmolarities (with lower Ea). Moreover, the initial inner volumes attained a maximum value for cells equilibrated in lower osmolarities (below 0.75 M) suggesting a preswollen state with the membrane under tension, independent of aquaporin expression. In this situation, the impairment of water channel activity suggested by lower Pf and higher Ea could probably be the first available volume regulatory tool that, in cooperation with other osmosensitive solute transporters, aims to maintain cell volume. The results presented point to the regulation of yeast water channels by membrane tension, as previously described in other cell systems |
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Membrane tension regulates water transport in yeastyeastSaccharomyces cerevisiaewater transport regulationaquaporinsmembrane tensionEvidence that membrane surface tension regulates water fluxes in intact cells of a Saccharomyces cerevisiae strain overexpressing aquaporin AQY1 was obtained by assessing the osmotic water transport parameters in cells equilibrated in different osmolarities. The osmotic water permeability coefficients (Pf) obtained for yeast cells overexpressing AQY1 incubated in low osmolarity buffers were similar to those obtained for a double mutant aqy1aqy2 and approximately three times lower (with higher activation energy, Ea) than values obtained for cells incubated in higher osmolarities (with lower Ea). Moreover, the initial inner volumes attained a maximum value for cells equilibrated in lower osmolarities (below 0.75 M) suggesting a preswollen state with the membrane under tension, independent of aquaporin expression. In this situation, the impairment of water channel activity suggested by lower Pf and higher Ea could probably be the first available volume regulatory tool that, in cooperation with other osmosensitive solute transporters, aims to maintain cell volume. The results presented point to the regulation of yeast water channels by membrane tension, as previously described in other cell systemsElsevierRepositório da Universidade de LisboaSoveral, GraçaMadeira, AnaLoureiro-Dias, Maria C.Moura, Teresa F.2013-07-10T16:02:53Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/5802por"Biochimica et Biophysica Acta". ISSN 0005-2736. 1778 (2008) 2573-25790005-2736info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:36:39Zoai:www.repository.utl.pt:10400.5/5802Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:53:14.251746Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Membrane tension regulates water transport in yeast |
title |
Membrane tension regulates water transport in yeast |
spellingShingle |
Membrane tension regulates water transport in yeast Soveral, Graça yeast Saccharomyces cerevisiae water transport regulation aquaporins membrane tension |
title_short |
Membrane tension regulates water transport in yeast |
title_full |
Membrane tension regulates water transport in yeast |
title_fullStr |
Membrane tension regulates water transport in yeast |
title_full_unstemmed |
Membrane tension regulates water transport in yeast |
title_sort |
Membrane tension regulates water transport in yeast |
author |
Soveral, Graça |
author_facet |
Soveral, Graça Madeira, Ana Loureiro-Dias, Maria C. Moura, Teresa F. |
author_role |
author |
author2 |
Madeira, Ana Loureiro-Dias, Maria C. Moura, Teresa F. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Soveral, Graça Madeira, Ana Loureiro-Dias, Maria C. Moura, Teresa F. |
dc.subject.por.fl_str_mv |
yeast Saccharomyces cerevisiae water transport regulation aquaporins membrane tension |
topic |
yeast Saccharomyces cerevisiae water transport regulation aquaporins membrane tension |
description |
Evidence that membrane surface tension regulates water fluxes in intact cells of a Saccharomyces cerevisiae strain overexpressing aquaporin AQY1 was obtained by assessing the osmotic water transport parameters in cells equilibrated in different osmolarities. The osmotic water permeability coefficients (Pf) obtained for yeast cells overexpressing AQY1 incubated in low osmolarity buffers were similar to those obtained for a double mutant aqy1aqy2 and approximately three times lower (with higher activation energy, Ea) than values obtained for cells incubated in higher osmolarities (with lower Ea). Moreover, the initial inner volumes attained a maximum value for cells equilibrated in lower osmolarities (below 0.75 M) suggesting a preswollen state with the membrane under tension, independent of aquaporin expression. In this situation, the impairment of water channel activity suggested by lower Pf and higher Ea could probably be the first available volume regulatory tool that, in cooperation with other osmosensitive solute transporters, aims to maintain cell volume. The results presented point to the regulation of yeast water channels by membrane tension, as previously described in other cell systems |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2008-01-01T00:00:00Z 2013-07-10T16:02:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/5802 |
url |
http://hdl.handle.net/10400.5/5802 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
"Biochimica et Biophysica Acta". ISSN 0005-2736. 1778 (2008) 2573-2579 0005-2736 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131008539295744 |