Polyphenols as resistance modulators in Arcobacter butzleri
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/6548 |
Resumo: | Arcobacter butzleri is an emerging human and animal pathogen for which an increased prevalence of resistance to antibiotics has been observed, and so alternative compounds to modulate resistance of A. butzleri are required. This work aims to study the potential use of several polyphenols as efflux pump inhibitors (EPIs) and to evaluate their interaction with antibiotics, in order to enhance antibiotic activity against A. butzleri. The minimum inhibitory concentration (MIC) of (-)-epicatechin, (+)-catechin, rutin, gallic acid, caffeic acid, chlorogenic acid, resveratrol, pterostilbene, and pinosylvin was determined, in absence and presence of four known EPIs. Subsequently, ethidium bromide accumulation in presence of subinhibitory concentrations of polyphenols was evaluated, and the synergistic potential of the compounds with antibiotics was assessed by checkerboard dilution test. Only stilbenes presented activity against A. butzleri, with MIC values ranging between 64 and 512 μg/mL. The MIC determination of the polyphenols in the presence of subinhibitory concentrations of known EPIs showed that efflux pumps play a role in the resistance to these compounds. Stilbenes also induced a higher intracellular accumulation of ethidium bromide, indicating that they may inhibit the activity of efflux pumps. Checkerboard assays showed that several combinations of polyphenol/antibiotic had an additive effect against A. butzleri. Overall, the results indicate that some polyphenols reduce A. butzleri resistance to antibiotics, suggesting the potential of stilbenes as EPIs. The potential of resveratrol and pinosylvin as resistance modulators was evidenced, insofar as these compounds can even revert antibiotic resistance. Therefore, the use of polyphenols as resistance modulators could be an alternative to overcome the decreasing susceptibility of A. butzleri to antibiotics. |
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Polyphenols as resistance modulators in Arcobacter butzleriAnti-Bacterial AgentsArcobacterBacterial ProteinsDrug Resistance, BacterialGram-Negative Bacterial InfectionsHumansMicrobial Sensitivity TestsPolyphenolsInfecções GastrointestinaisResistência aos AntimicrobianosArcobacter butzleri is an emerging human and animal pathogen for which an increased prevalence of resistance to antibiotics has been observed, and so alternative compounds to modulate resistance of A. butzleri are required. This work aims to study the potential use of several polyphenols as efflux pump inhibitors (EPIs) and to evaluate their interaction with antibiotics, in order to enhance antibiotic activity against A. butzleri. The minimum inhibitory concentration (MIC) of (-)-epicatechin, (+)-catechin, rutin, gallic acid, caffeic acid, chlorogenic acid, resveratrol, pterostilbene, and pinosylvin was determined, in absence and presence of four known EPIs. Subsequently, ethidium bromide accumulation in presence of subinhibitory concentrations of polyphenols was evaluated, and the synergistic potential of the compounds with antibiotics was assessed by checkerboard dilution test. Only stilbenes presented activity against A. butzleri, with MIC values ranging between 64 and 512 μg/mL. The MIC determination of the polyphenols in the presence of subinhibitory concentrations of known EPIs showed that efflux pumps play a role in the resistance to these compounds. Stilbenes also induced a higher intracellular accumulation of ethidium bromide, indicating that they may inhibit the activity of efflux pumps. Checkerboard assays showed that several combinations of polyphenol/antibiotic had an additive effect against A. butzleri. Overall, the results indicate that some polyphenols reduce A. butzleri resistance to antibiotics, suggesting the potential of stilbenes as EPIs. The potential of resveratrol and pinosylvin as resistance modulators was evidenced, insofar as these compounds can even revert antibiotic resistance. Therefore, the use of polyphenols as resistance modulators could be an alternative to overcome the decreasing susceptibility of A. butzleri to antibiotics.Susana Ferreira was supported by a fellowship (SFRH/BPD/101959/2014) from Fundação para a Ciência e Tecnologia and co-financed by Fundo Social Europeu. This work was supported by FEDER funds through the POCI - COMPETE 2020 - Operational Programme Competitiveness and Internationalization in Axis I— Strengthening research, technological development, and innovation (Project POCI-01-0145-FEDER-007491) and National Funds by FCT - Foundation for Science and Technology (Project UID/Multi/00709/2013).Springer Verlag/ Institute of Microbiology, Academy of Sciences of the Czech RepublicRepositório Científico do Instituto Nacional de SaúdeSousa, VanessaLuís, ÂngeloOleastro, MónicaDomingues, FernandaFerreira, Susana2020-04-29T04:46:13Z2019-072019-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6548engFolia Microbiol (Praha). 2019 Jul;64(4):547-554. doi: 10.1007/s12223-019-00678-3. Epub 2019 Jan 130015-563210.1007/s12223-019-00678-3info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:43Zoai:repositorio.insa.pt:10400.18/6548Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:34.078395Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Polyphenols as resistance modulators in Arcobacter butzleri |
title |
Polyphenols as resistance modulators in Arcobacter butzleri |
spellingShingle |
Polyphenols as resistance modulators in Arcobacter butzleri Sousa, Vanessa Anti-Bacterial Agents Arcobacter Bacterial Proteins Drug Resistance, Bacterial Gram-Negative Bacterial Infections Humans Microbial Sensitivity Tests Polyphenols Infecções Gastrointestinais Resistência aos Antimicrobianos |
title_short |
Polyphenols as resistance modulators in Arcobacter butzleri |
title_full |
Polyphenols as resistance modulators in Arcobacter butzleri |
title_fullStr |
Polyphenols as resistance modulators in Arcobacter butzleri |
title_full_unstemmed |
Polyphenols as resistance modulators in Arcobacter butzleri |
title_sort |
Polyphenols as resistance modulators in Arcobacter butzleri |
author |
Sousa, Vanessa |
author_facet |
Sousa, Vanessa Luís, Ângelo Oleastro, Mónica Domingues, Fernanda Ferreira, Susana |
author_role |
author |
author2 |
Luís, Ângelo Oleastro, Mónica Domingues, Fernanda Ferreira, Susana |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Sousa, Vanessa Luís, Ângelo Oleastro, Mónica Domingues, Fernanda Ferreira, Susana |
dc.subject.por.fl_str_mv |
Anti-Bacterial Agents Arcobacter Bacterial Proteins Drug Resistance, Bacterial Gram-Negative Bacterial Infections Humans Microbial Sensitivity Tests Polyphenols Infecções Gastrointestinais Resistência aos Antimicrobianos |
topic |
Anti-Bacterial Agents Arcobacter Bacterial Proteins Drug Resistance, Bacterial Gram-Negative Bacterial Infections Humans Microbial Sensitivity Tests Polyphenols Infecções Gastrointestinais Resistência aos Antimicrobianos |
description |
Arcobacter butzleri is an emerging human and animal pathogen for which an increased prevalence of resistance to antibiotics has been observed, and so alternative compounds to modulate resistance of A. butzleri are required. This work aims to study the potential use of several polyphenols as efflux pump inhibitors (EPIs) and to evaluate their interaction with antibiotics, in order to enhance antibiotic activity against A. butzleri. The minimum inhibitory concentration (MIC) of (-)-epicatechin, (+)-catechin, rutin, gallic acid, caffeic acid, chlorogenic acid, resveratrol, pterostilbene, and pinosylvin was determined, in absence and presence of four known EPIs. Subsequently, ethidium bromide accumulation in presence of subinhibitory concentrations of polyphenols was evaluated, and the synergistic potential of the compounds with antibiotics was assessed by checkerboard dilution test. Only stilbenes presented activity against A. butzleri, with MIC values ranging between 64 and 512 μg/mL. The MIC determination of the polyphenols in the presence of subinhibitory concentrations of known EPIs showed that efflux pumps play a role in the resistance to these compounds. Stilbenes also induced a higher intracellular accumulation of ethidium bromide, indicating that they may inhibit the activity of efflux pumps. Checkerboard assays showed that several combinations of polyphenol/antibiotic had an additive effect against A. butzleri. Overall, the results indicate that some polyphenols reduce A. butzleri resistance to antibiotics, suggesting the potential of stilbenes as EPIs. The potential of resveratrol and pinosylvin as resistance modulators was evidenced, insofar as these compounds can even revert antibiotic resistance. Therefore, the use of polyphenols as resistance modulators could be an alternative to overcome the decreasing susceptibility of A. butzleri to antibiotics. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07 2019-07-01T00:00:00Z 2020-04-29T04:46:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/6548 |
url |
http://hdl.handle.net/10400.18/6548 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Folia Microbiol (Praha). 2019 Jul;64(4):547-554. doi: 10.1007/s12223-019-00678-3. Epub 2019 Jan 13 0015-5632 10.1007/s12223-019-00678-3 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Verlag/ Institute of Microbiology, Academy of Sciences of the Czech Republic |
publisher.none.fl_str_mv |
Springer Verlag/ Institute of Microbiology, Academy of Sciences of the Czech Republic |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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