Polyphenols as resistance modulators in Arcobacter butzleri

Detalhes bibliográficos
Autor(a) principal: Sousa, Vanessa
Data de Publicação: 2019
Outros Autores: Luís, Ângelo, Oleastro, Mónica, Domingues, Fernanda, Ferreira, Susana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/6548
Resumo: Arcobacter butzleri is an emerging human and animal pathogen for which an increased prevalence of resistance to antibiotics has been observed, and so alternative compounds to modulate resistance of A. butzleri are required. This work aims to study the potential use of several polyphenols as efflux pump inhibitors (EPIs) and to evaluate their interaction with antibiotics, in order to enhance antibiotic activity against A. butzleri. The minimum inhibitory concentration (MIC) of (-)-epicatechin, (+)-catechin, rutin, gallic acid, caffeic acid, chlorogenic acid, resveratrol, pterostilbene, and pinosylvin was determined, in absence and presence of four known EPIs. Subsequently, ethidium bromide accumulation in presence of subinhibitory concentrations of polyphenols was evaluated, and the synergistic potential of the compounds with antibiotics was assessed by checkerboard dilution test. Only stilbenes presented activity against A. butzleri, with MIC values ranging between 64 and 512 μg/mL. The MIC determination of the polyphenols in the presence of subinhibitory concentrations of known EPIs showed that efflux pumps play a role in the resistance to these compounds. Stilbenes also induced a higher intracellular accumulation of ethidium bromide, indicating that they may inhibit the activity of efflux pumps. Checkerboard assays showed that several combinations of polyphenol/antibiotic had an additive effect against A. butzleri. Overall, the results indicate that some polyphenols reduce A. butzleri resistance to antibiotics, suggesting the potential of stilbenes as EPIs. The potential of resveratrol and pinosylvin as resistance modulators was evidenced, insofar as these compounds can even revert antibiotic resistance. Therefore, the use of polyphenols as resistance modulators could be an alternative to overcome the decreasing susceptibility of A. butzleri to antibiotics.
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spelling Polyphenols as resistance modulators in Arcobacter butzleriAnti-Bacterial AgentsArcobacterBacterial ProteinsDrug Resistance, BacterialGram-Negative Bacterial InfectionsHumansMicrobial Sensitivity TestsPolyphenolsInfecções GastrointestinaisResistência aos AntimicrobianosArcobacter butzleri is an emerging human and animal pathogen for which an increased prevalence of resistance to antibiotics has been observed, and so alternative compounds to modulate resistance of A. butzleri are required. This work aims to study the potential use of several polyphenols as efflux pump inhibitors (EPIs) and to evaluate their interaction with antibiotics, in order to enhance antibiotic activity against A. butzleri. The minimum inhibitory concentration (MIC) of (-)-epicatechin, (+)-catechin, rutin, gallic acid, caffeic acid, chlorogenic acid, resveratrol, pterostilbene, and pinosylvin was determined, in absence and presence of four known EPIs. Subsequently, ethidium bromide accumulation in presence of subinhibitory concentrations of polyphenols was evaluated, and the synergistic potential of the compounds with antibiotics was assessed by checkerboard dilution test. Only stilbenes presented activity against A. butzleri, with MIC values ranging between 64 and 512 μg/mL. The MIC determination of the polyphenols in the presence of subinhibitory concentrations of known EPIs showed that efflux pumps play a role in the resistance to these compounds. Stilbenes also induced a higher intracellular accumulation of ethidium bromide, indicating that they may inhibit the activity of efflux pumps. Checkerboard assays showed that several combinations of polyphenol/antibiotic had an additive effect against A. butzleri. Overall, the results indicate that some polyphenols reduce A. butzleri resistance to antibiotics, suggesting the potential of stilbenes as EPIs. The potential of resveratrol and pinosylvin as resistance modulators was evidenced, insofar as these compounds can even revert antibiotic resistance. Therefore, the use of polyphenols as resistance modulators could be an alternative to overcome the decreasing susceptibility of A. butzleri to antibiotics.Susana Ferreira was supported by a fellowship (SFRH/BPD/101959/2014) from Fundação para a Ciência e Tecnologia and co-financed by Fundo Social Europeu. This work was supported by FEDER funds through the POCI - COMPETE 2020 - Operational Programme Competitiveness and Internationalization in Axis I— Strengthening research, technological development, and innovation (Project POCI-01-0145-FEDER-007491) and National Funds by FCT - Foundation for Science and Technology (Project UID/Multi/00709/2013).Springer Verlag/ Institute of Microbiology, Academy of Sciences of the Czech RepublicRepositório Científico do Instituto Nacional de SaúdeSousa, VanessaLuís, ÂngeloOleastro, MónicaDomingues, FernandaFerreira, Susana2020-04-29T04:46:13Z2019-072019-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6548engFolia Microbiol (Praha). 2019 Jul;64(4):547-554. doi: 10.1007/s12223-019-00678-3. Epub 2019 Jan 130015-563210.1007/s12223-019-00678-3info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:43Zoai:repositorio.insa.pt:10400.18/6548Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:34.078395Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Polyphenols as resistance modulators in Arcobacter butzleri
title Polyphenols as resistance modulators in Arcobacter butzleri
spellingShingle Polyphenols as resistance modulators in Arcobacter butzleri
Sousa, Vanessa
Anti-Bacterial Agents
Arcobacter
Bacterial Proteins
Drug Resistance, Bacterial
Gram-Negative Bacterial Infections
Humans
Microbial Sensitivity Tests
Polyphenols
Infecções Gastrointestinais
Resistência aos Antimicrobianos
title_short Polyphenols as resistance modulators in Arcobacter butzleri
title_full Polyphenols as resistance modulators in Arcobacter butzleri
title_fullStr Polyphenols as resistance modulators in Arcobacter butzleri
title_full_unstemmed Polyphenols as resistance modulators in Arcobacter butzleri
title_sort Polyphenols as resistance modulators in Arcobacter butzleri
author Sousa, Vanessa
author_facet Sousa, Vanessa
Luís, Ângelo
Oleastro, Mónica
Domingues, Fernanda
Ferreira, Susana
author_role author
author2 Luís, Ângelo
Oleastro, Mónica
Domingues, Fernanda
Ferreira, Susana
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Sousa, Vanessa
Luís, Ângelo
Oleastro, Mónica
Domingues, Fernanda
Ferreira, Susana
dc.subject.por.fl_str_mv Anti-Bacterial Agents
Arcobacter
Bacterial Proteins
Drug Resistance, Bacterial
Gram-Negative Bacterial Infections
Humans
Microbial Sensitivity Tests
Polyphenols
Infecções Gastrointestinais
Resistência aos Antimicrobianos
topic Anti-Bacterial Agents
Arcobacter
Bacterial Proteins
Drug Resistance, Bacterial
Gram-Negative Bacterial Infections
Humans
Microbial Sensitivity Tests
Polyphenols
Infecções Gastrointestinais
Resistência aos Antimicrobianos
description Arcobacter butzleri is an emerging human and animal pathogen for which an increased prevalence of resistance to antibiotics has been observed, and so alternative compounds to modulate resistance of A. butzleri are required. This work aims to study the potential use of several polyphenols as efflux pump inhibitors (EPIs) and to evaluate their interaction with antibiotics, in order to enhance antibiotic activity against A. butzleri. The minimum inhibitory concentration (MIC) of (-)-epicatechin, (+)-catechin, rutin, gallic acid, caffeic acid, chlorogenic acid, resveratrol, pterostilbene, and pinosylvin was determined, in absence and presence of four known EPIs. Subsequently, ethidium bromide accumulation in presence of subinhibitory concentrations of polyphenols was evaluated, and the synergistic potential of the compounds with antibiotics was assessed by checkerboard dilution test. Only stilbenes presented activity against A. butzleri, with MIC values ranging between 64 and 512 μg/mL. The MIC determination of the polyphenols in the presence of subinhibitory concentrations of known EPIs showed that efflux pumps play a role in the resistance to these compounds. Stilbenes also induced a higher intracellular accumulation of ethidium bromide, indicating that they may inhibit the activity of efflux pumps. Checkerboard assays showed that several combinations of polyphenol/antibiotic had an additive effect against A. butzleri. Overall, the results indicate that some polyphenols reduce A. butzleri resistance to antibiotics, suggesting the potential of stilbenes as EPIs. The potential of resveratrol and pinosylvin as resistance modulators was evidenced, insofar as these compounds can even revert antibiotic resistance. Therefore, the use of polyphenols as resistance modulators could be an alternative to overcome the decreasing susceptibility of A. butzleri to antibiotics.
publishDate 2019
dc.date.none.fl_str_mv 2019-07
2019-07-01T00:00:00Z
2020-04-29T04:46:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/6548
url http://hdl.handle.net/10400.18/6548
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Folia Microbiol (Praha). 2019 Jul;64(4):547-554. doi: 10.1007/s12223-019-00678-3. Epub 2019 Jan 13
0015-5632
10.1007/s12223-019-00678-3
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag/ Institute of Microbiology, Academy of Sciences of the Czech Republic
publisher.none.fl_str_mv Springer Verlag/ Institute of Microbiology, Academy of Sciences of the Czech Republic
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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