Maternal dietary exposure to mycotoxin aflatoxin B1 promotes intestinal immune alterations and microbiota modifications increasing infection susceptibility in mouse offspring

Detalhes bibliográficos
Autor(a) principal: Bastos-Amador, Patricia
Data de Publicação: 2023
Outros Autores: Duarte, Elsa Leclerc, Torres, Júlio, Caldeira, Ana Teresa, Silva, Inês, Salvador, Cátia, Assunção, Ricardo, Alvito, Paula, Ferreira, Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8663
Resumo: Abstract: Mycotoxins are secondary metabolites produced by fungi occurring in food that are toxic to animals and humans. Early-life mycotoxins exposure has been linked to diverse pathologies. However, how maternal exposure to mycotoxins impacts on the intestinal barrier function of progeny has not been explored. Here, exposure of pregnant and lactating C57Bl/6J female mice to aflatoxin B1 (AFB1; 400 μg/kg body weight/day; 3 times a week) in gelatine pellets, from embryonic day (E)11.5 until weaning (postnatal day 21), led to gut immunological changes in progeny. The results showed an overall increase of lymphocyte number in intestine, a reduction of expression of epithelial genes related to microbial defence, as well as a decrease in cytokine production by intestinal type 2 innate lymphoid cells (ILC2). While susceptibility to chemically induced colitis was not worsened, immune alterations were associated with changes in gut microbiota and with a higher vulnerability to infection by the protozoan Eimeria vermiformis at early-life. Together these results show that maternal dietary exposure to AFB1 can dampen intestinal barrier homeostasis in offspring decreasing their capability to tackle intestinal pathogens. These data provide insights to understand AFB1 potential harmfulness in early-life health in the context of intestinal infections.
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spelling Maternal dietary exposure to mycotoxin aflatoxin B1 promotes intestinal immune alterations and microbiota modifications increasing infection susceptibility in mouse offspringMycotoxinsEarly-life ExposureMaternal Dietary ExposureIntestinal Immune SystemMicotoxinasMicrobiotaExposição PrecoceSegurança AlimentarToxicologiaAbstract: Mycotoxins are secondary metabolites produced by fungi occurring in food that are toxic to animals and humans. Early-life mycotoxins exposure has been linked to diverse pathologies. However, how maternal exposure to mycotoxins impacts on the intestinal barrier function of progeny has not been explored. Here, exposure of pregnant and lactating C57Bl/6J female mice to aflatoxin B1 (AFB1; 400 μg/kg body weight/day; 3 times a week) in gelatine pellets, from embryonic day (E)11.5 until weaning (postnatal day 21), led to gut immunological changes in progeny. The results showed an overall increase of lymphocyte number in intestine, a reduction of expression of epithelial genes related to microbial defence, as well as a decrease in cytokine production by intestinal type 2 innate lymphoid cells (ILC2). While susceptibility to chemically induced colitis was not worsened, immune alterations were associated with changes in gut microbiota and with a higher vulnerability to infection by the protozoan Eimeria vermiformis at early-life. Together these results show that maternal dietary exposure to AFB1 can dampen intestinal barrier homeostasis in offspring decreasing their capability to tackle intestinal pathogens. These data provide insights to understand AFB1 potential harmfulness in early-life health in the context of intestinal infections.Highlights: Effect of maternal AFB1 dietary exposure was studied in intestinal mucosa of offspring mice; Maternal AFB1 exposure leads to lymphocyte expansion and alters cytokine expression; Maternal AFB1 exposure reduces microbial-related epithelial gene expression in offspring; Maternal AFB1 exposure modifies offspring gut microbiota.; Maternal AFB1-exposed offspring are more vulnerable to intestinal parasite infection.EarlyMyco (PTDC/MED-TOX/28762/ 2017), investigator FCT Exploratory (IF/00425/2015/CP1324/CT0001) and CESAM (UIDP/50017/2020+UIDB/50017/2020+LA/P/0094/ 2020)ElsevierRepositório Científico do Instituto Nacional de SaúdeBastos-Amador, PatriciaDuarte, Elsa LeclercTorres, JúlioCaldeira, Ana TeresaSilva, InêsSalvador, CátiaAssunção, RicardoAlvito, PaulaFerreira, Manuela2023-10-06T10:50:12Z2023-01-022023-01-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8663engFood Chem Toxicol. 2023 Mar:173:113596. doi: 10.1016/j.fct.2022.113596. Epub 2023 Jan 2.0278-691510.1016/j.fct.2022.113596info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-14T01:31:32Zoai:repositorio.insa.pt:10400.18/8663Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:33:33.847331Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Maternal dietary exposure to mycotoxin aflatoxin B1 promotes intestinal immune alterations and microbiota modifications increasing infection susceptibility in mouse offspring
title Maternal dietary exposure to mycotoxin aflatoxin B1 promotes intestinal immune alterations and microbiota modifications increasing infection susceptibility in mouse offspring
spellingShingle Maternal dietary exposure to mycotoxin aflatoxin B1 promotes intestinal immune alterations and microbiota modifications increasing infection susceptibility in mouse offspring
Bastos-Amador, Patricia
Mycotoxins
Early-life Exposure
Maternal Dietary Exposure
Intestinal Immune System
Micotoxinas
Microbiota
Exposição Precoce
Segurança Alimentar
Toxicologia
title_short Maternal dietary exposure to mycotoxin aflatoxin B1 promotes intestinal immune alterations and microbiota modifications increasing infection susceptibility in mouse offspring
title_full Maternal dietary exposure to mycotoxin aflatoxin B1 promotes intestinal immune alterations and microbiota modifications increasing infection susceptibility in mouse offspring
title_fullStr Maternal dietary exposure to mycotoxin aflatoxin B1 promotes intestinal immune alterations and microbiota modifications increasing infection susceptibility in mouse offspring
title_full_unstemmed Maternal dietary exposure to mycotoxin aflatoxin B1 promotes intestinal immune alterations and microbiota modifications increasing infection susceptibility in mouse offspring
title_sort Maternal dietary exposure to mycotoxin aflatoxin B1 promotes intestinal immune alterations and microbiota modifications increasing infection susceptibility in mouse offspring
author Bastos-Amador, Patricia
author_facet Bastos-Amador, Patricia
Duarte, Elsa Leclerc
Torres, Júlio
Caldeira, Ana Teresa
Silva, Inês
Salvador, Cátia
Assunção, Ricardo
Alvito, Paula
Ferreira, Manuela
author_role author
author2 Duarte, Elsa Leclerc
Torres, Júlio
Caldeira, Ana Teresa
Silva, Inês
Salvador, Cátia
Assunção, Ricardo
Alvito, Paula
Ferreira, Manuela
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Bastos-Amador, Patricia
Duarte, Elsa Leclerc
Torres, Júlio
Caldeira, Ana Teresa
Silva, Inês
Salvador, Cátia
Assunção, Ricardo
Alvito, Paula
Ferreira, Manuela
dc.subject.por.fl_str_mv Mycotoxins
Early-life Exposure
Maternal Dietary Exposure
Intestinal Immune System
Micotoxinas
Microbiota
Exposição Precoce
Segurança Alimentar
Toxicologia
topic Mycotoxins
Early-life Exposure
Maternal Dietary Exposure
Intestinal Immune System
Micotoxinas
Microbiota
Exposição Precoce
Segurança Alimentar
Toxicologia
description Abstract: Mycotoxins are secondary metabolites produced by fungi occurring in food that are toxic to animals and humans. Early-life mycotoxins exposure has been linked to diverse pathologies. However, how maternal exposure to mycotoxins impacts on the intestinal barrier function of progeny has not been explored. Here, exposure of pregnant and lactating C57Bl/6J female mice to aflatoxin B1 (AFB1; 400 μg/kg body weight/day; 3 times a week) in gelatine pellets, from embryonic day (E)11.5 until weaning (postnatal day 21), led to gut immunological changes in progeny. The results showed an overall increase of lymphocyte number in intestine, a reduction of expression of epithelial genes related to microbial defence, as well as a decrease in cytokine production by intestinal type 2 innate lymphoid cells (ILC2). While susceptibility to chemically induced colitis was not worsened, immune alterations were associated with changes in gut microbiota and with a higher vulnerability to infection by the protozoan Eimeria vermiformis at early-life. Together these results show that maternal dietary exposure to AFB1 can dampen intestinal barrier homeostasis in offspring decreasing their capability to tackle intestinal pathogens. These data provide insights to understand AFB1 potential harmfulness in early-life health in the context of intestinal infections.
publishDate 2023
dc.date.none.fl_str_mv 2023-10-06T10:50:12Z
2023-01-02
2023-01-02T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8663
url http://hdl.handle.net/10400.18/8663
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Food Chem Toxicol. 2023 Mar:173:113596. doi: 10.1016/j.fct.2022.113596. Epub 2023 Jan 2.
0278-6915
10.1016/j.fct.2022.113596
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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