Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo de conferência |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/28318 |
Resumo: | Grapevine trunk diseases (GTDs) are one of the most important fungal diseases affecting grapevine plants in all the major growing regions of the world (Pintos et al, 2018)1 with up to 133 fungal species belonging to 34 genera associated to this disease (Gemaje et al, 2018)2. Management of GTDs is difficult and its complete eradication is not possible. For these reasons, its control is meanly focused on disease prevention and mitigation (U´rbez-Torres 2011)3.The work here presented had the aim to identify molecularly the endophytic fungi present in different tissues of grapevines, and among them the fungi responsible for GTDs. Pure fungal cultures were isolated from diseased grapevines plants belonging to two cultivars of Alentejo region (‘Trincadeira’ and ‘Alicante Bouchet’). Mycelium was then harvested, gDNA was extracted (Doyle and Doyle, 1987), and for fungal genetic classification the ribosomal internal transcribed spacer (ITS) region of nuclear rDNA was amplified through PCR by using ITS1 and ITS4 primers (White et al. 1990)4. All the fungi sequences that showed resemblance of 98% were used to classify the fungus analysed. The most prevalent grapevine trunk disease fungi identified were Hormonema viticola, Stereum armeniaccum, Philophorafastigiata, Diaporthe, Truncatella angustata, Cytospora acaciae, Diplodia pseudoseriata, Neofusicocum parvum, and Pestalotiopsis sp. |
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Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Regiongrapevine trunk diseaseVivis viniferafungal genetic classificationITSGrapevine trunk diseases (GTDs) are one of the most important fungal diseases affecting grapevine plants in all the major growing regions of the world (Pintos et al, 2018)1 with up to 133 fungal species belonging to 34 genera associated to this disease (Gemaje et al, 2018)2. Management of GTDs is difficult and its complete eradication is not possible. For these reasons, its control is meanly focused on disease prevention and mitigation (U´rbez-Torres 2011)3.The work here presented had the aim to identify molecularly the endophytic fungi present in different tissues of grapevines, and among them the fungi responsible for GTDs. Pure fungal cultures were isolated from diseased grapevines plants belonging to two cultivars of Alentejo region (‘Trincadeira’ and ‘Alicante Bouchet’). Mycelium was then harvested, gDNA was extracted (Doyle and Doyle, 1987), and for fungal genetic classification the ribosomal internal transcribed spacer (ITS) region of nuclear rDNA was amplified through PCR by using ITS1 and ITS4 primers (White et al. 1990)4. All the fungi sequences that showed resemblance of 98% were used to classify the fungus analysed. The most prevalent grapevine trunk disease fungi identified were Hormonema viticola, Stereum armeniaccum, Philophorafastigiata, Diaporthe, Truncatella angustata, Cytospora acaciae, Diplodia pseudoseriata, Neofusicocum parvum, and Pestalotiopsis sp.2020-11-03T15:55:32Z2020-11-032019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/28318http://hdl.handle.net/10174/28318porde Almeida, A.B.; Concas, J.; Campos, M.D. , Varanda, C.M.R.; Materatski, P.; Murolo, S.; Romanazzi, G.; Félix, M.R. Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region. (2019). I Jornadas MED (Mediterranean Institute for Agriculture, Environment and Development). 27-28 June, Évora, Portugal, Book of abstracts, p. 117.naonaosimmdcc@uevora.ptcarlavaranda@uevora.ptpmateratski@uevora.ptmrff@uevora.pt581Campos, Maria DoroteiaVaranda, CarlaMateratski, PatrickFélix, Maria Rosárioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:23:59Zoai:dspace.uevora.pt:10174/28318Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:17:59.303035Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region |
title |
Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region |
spellingShingle |
Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region Campos, Maria Doroteia grapevine trunk disease Vivis vinifera fungal genetic classification ITS |
title_short |
Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region |
title_full |
Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region |
title_fullStr |
Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region |
title_full_unstemmed |
Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region |
title_sort |
Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region |
author |
Campos, Maria Doroteia |
author_facet |
Campos, Maria Doroteia Varanda, Carla Materatski, Patrick Félix, Maria Rosário |
author_role |
author |
author2 |
Varanda, Carla Materatski, Patrick Félix, Maria Rosário |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Campos, Maria Doroteia Varanda, Carla Materatski, Patrick Félix, Maria Rosário |
dc.subject.por.fl_str_mv |
grapevine trunk disease Vivis vinifera fungal genetic classification ITS |
topic |
grapevine trunk disease Vivis vinifera fungal genetic classification ITS |
description |
Grapevine trunk diseases (GTDs) are one of the most important fungal diseases affecting grapevine plants in all the major growing regions of the world (Pintos et al, 2018)1 with up to 133 fungal species belonging to 34 genera associated to this disease (Gemaje et al, 2018)2. Management of GTDs is difficult and its complete eradication is not possible. For these reasons, its control is meanly focused on disease prevention and mitigation (U´rbez-Torres 2011)3.The work here presented had the aim to identify molecularly the endophytic fungi present in different tissues of grapevines, and among them the fungi responsible for GTDs. Pure fungal cultures were isolated from diseased grapevines plants belonging to two cultivars of Alentejo region (‘Trincadeira’ and ‘Alicante Bouchet’). Mycelium was then harvested, gDNA was extracted (Doyle and Doyle, 1987), and for fungal genetic classification the ribosomal internal transcribed spacer (ITS) region of nuclear rDNA was amplified through PCR by using ITS1 and ITS4 primers (White et al. 1990)4. All the fungi sequences that showed resemblance of 98% were used to classify the fungus analysed. The most prevalent grapevine trunk disease fungi identified were Hormonema viticola, Stereum armeniaccum, Philophorafastigiata, Diaporthe, Truncatella angustata, Cytospora acaciae, Diplodia pseudoseriata, Neofusicocum parvum, and Pestalotiopsis sp. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01T00:00:00Z 2020-11-03T15:55:32Z 2020-11-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/conferenceObject |
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conferenceObject |
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publishedVersion |
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http://hdl.handle.net/10174/28318 http://hdl.handle.net/10174/28318 |
url |
http://hdl.handle.net/10174/28318 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
de Almeida, A.B.; Concas, J.; Campos, M.D. , Varanda, C.M.R.; Materatski, P.; Murolo, S.; Romanazzi, G.; Félix, M.R. Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region. (2019). I Jornadas MED (Mediterranean Institute for Agriculture, Environment and Development). 27-28 June, Évora, Portugal, Book of abstracts, p. 117. nao nao sim mdcc@uevora.pt carlavaranda@uevora.pt pmateratski@uevora.pt mrff@uevora.pt 581 |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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