Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region

Detalhes bibliográficos
Autor(a) principal: Campos, Maria Doroteia
Data de Publicação: 2019
Outros Autores: Varanda, Carla, Materatski, Patrick, Félix, Maria Rosário
Tipo de documento: Artigo de conferência
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/28318
Resumo: Grapevine trunk diseases (GTDs) are one of the most important fungal diseases affecting grapevine plants in all the major growing regions of the world (Pintos et al, 2018)1 with up to 133 fungal species belonging to 34 genera associated to this disease (Gemaje et al, 2018)2. Management of GTDs is difficult and its complete eradication is not possible. For these reasons, its control is meanly focused on disease prevention and mitigation (U´rbez-Torres 2011)3.The work here presented had the aim to identify molecularly the endophytic fungi present in different tissues of grapevines, and among them the fungi responsible for GTDs. Pure fungal cultures were isolated from diseased grapevines plants belonging to two cultivars of Alentejo region (‘Trincadeira’ and ‘Alicante Bouchet’). Mycelium was then harvested, gDNA was extracted (Doyle and Doyle, 1987), and for fungal genetic classification the ribosomal internal transcribed spacer (ITS) region of nuclear rDNA was amplified through PCR by using ITS1 and ITS4 primers (White et al. 1990)4. All the fungi sequences that showed resemblance of 98% were used to classify the fungus analysed. The most prevalent grapevine trunk disease fungi identified were Hormonema viticola, Stereum armeniaccum, Philophorafastigiata, Diaporthe, Truncatella angustata, Cytospora acaciae, Diplodia pseudoseriata, Neofusicocum parvum, and Pestalotiopsis sp.
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spelling Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Regiongrapevine trunk diseaseVivis viniferafungal genetic classificationITSGrapevine trunk diseases (GTDs) are one of the most important fungal diseases affecting grapevine plants in all the major growing regions of the world (Pintos et al, 2018)1 with up to 133 fungal species belonging to 34 genera associated to this disease (Gemaje et al, 2018)2. Management of GTDs is difficult and its complete eradication is not possible. For these reasons, its control is meanly focused on disease prevention and mitigation (U´rbez-Torres 2011)3.The work here presented had the aim to identify molecularly the endophytic fungi present in different tissues of grapevines, and among them the fungi responsible for GTDs. Pure fungal cultures were isolated from diseased grapevines plants belonging to two cultivars of Alentejo region (‘Trincadeira’ and ‘Alicante Bouchet’). Mycelium was then harvested, gDNA was extracted (Doyle and Doyle, 1987), and for fungal genetic classification the ribosomal internal transcribed spacer (ITS) region of nuclear rDNA was amplified through PCR by using ITS1 and ITS4 primers (White et al. 1990)4. All the fungi sequences that showed resemblance of 98% were used to classify the fungus analysed. The most prevalent grapevine trunk disease fungi identified were Hormonema viticola, Stereum armeniaccum, Philophorafastigiata, Diaporthe, Truncatella angustata, Cytospora acaciae, Diplodia pseudoseriata, Neofusicocum parvum, and Pestalotiopsis sp.2020-11-03T15:55:32Z2020-11-032019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/28318http://hdl.handle.net/10174/28318porde Almeida, A.B.; Concas, J.; Campos, M.D. , Varanda, C.M.R.; Materatski, P.; Murolo, S.; Romanazzi, G.; Félix, M.R. Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region. (2019). I Jornadas MED (Mediterranean Institute for Agriculture, Environment and Development). 27-28 June, Évora, Portugal, Book of abstracts, p. 117.naonaosimmdcc@uevora.ptcarlavaranda@uevora.ptpmateratski@uevora.ptmrff@uevora.pt581Campos, Maria DoroteiaVaranda, CarlaMateratski, PatrickFélix, Maria Rosárioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:23:59Zoai:dspace.uevora.pt:10174/28318Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:17:59.303035Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region
title Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region
spellingShingle Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region
Campos, Maria Doroteia
grapevine trunk disease
Vivis vinifera
fungal genetic classification
ITS
title_short Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region
title_full Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region
title_fullStr Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region
title_full_unstemmed Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region
title_sort Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region
author Campos, Maria Doroteia
author_facet Campos, Maria Doroteia
Varanda, Carla
Materatski, Patrick
Félix, Maria Rosário
author_role author
author2 Varanda, Carla
Materatski, Patrick
Félix, Maria Rosário
author2_role author
author
author
dc.contributor.author.fl_str_mv Campos, Maria Doroteia
Varanda, Carla
Materatski, Patrick
Félix, Maria Rosário
dc.subject.por.fl_str_mv grapevine trunk disease
Vivis vinifera
fungal genetic classification
ITS
topic grapevine trunk disease
Vivis vinifera
fungal genetic classification
ITS
description Grapevine trunk diseases (GTDs) are one of the most important fungal diseases affecting grapevine plants in all the major growing regions of the world (Pintos et al, 2018)1 with up to 133 fungal species belonging to 34 genera associated to this disease (Gemaje et al, 2018)2. Management of GTDs is difficult and its complete eradication is not possible. For these reasons, its control is meanly focused on disease prevention and mitigation (U´rbez-Torres 2011)3.The work here presented had the aim to identify molecularly the endophytic fungi present in different tissues of grapevines, and among them the fungi responsible for GTDs. Pure fungal cultures were isolated from diseased grapevines plants belonging to two cultivars of Alentejo region (‘Trincadeira’ and ‘Alicante Bouchet’). Mycelium was then harvested, gDNA was extracted (Doyle and Doyle, 1987), and for fungal genetic classification the ribosomal internal transcribed spacer (ITS) region of nuclear rDNA was amplified through PCR by using ITS1 and ITS4 primers (White et al. 1990)4. All the fungi sequences that showed resemblance of 98% were used to classify the fungus analysed. The most prevalent grapevine trunk disease fungi identified were Hormonema viticola, Stereum armeniaccum, Philophorafastigiata, Diaporthe, Truncatella angustata, Cytospora acaciae, Diplodia pseudoseriata, Neofusicocum parvum, and Pestalotiopsis sp.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01T00:00:00Z
2020-11-03T15:55:32Z
2020-11-03
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/28318
http://hdl.handle.net/10174/28318
url http://hdl.handle.net/10174/28318
dc.language.iso.fl_str_mv por
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dc.relation.none.fl_str_mv de Almeida, A.B.; Concas, J.; Campos, M.D. , Varanda, C.M.R.; Materatski, P.; Murolo, S.; Romanazzi, G.; Félix, M.R. Identification of Fungi Responsible for Grapevine Trunk Disease in Alentejo Region. (2019). I Jornadas MED (Mediterranean Institute for Agriculture, Environment and Development). 27-28 June, Évora, Portugal, Book of abstracts, p. 117.
nao
nao
sim
mdcc@uevora.pt
carlavaranda@uevora.pt
pmateratski@uevora.pt
mrff@uevora.pt
581
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