Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticles

Detalhes bibliográficos
Autor(a) principal: Braz, Luis
Data de Publicação: 2018
Outros Autores: Grenha, Ana, Corvo, Marta C., Lourenço, João P., Ferreira, Domingos, Sarmento, Bruno, Costa, Ana M. Rosa da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/10376
Resumo: The development of LBG-based nanoparticles intending an application in oral immunization is presented. Nanoparticle production occurred by mild polyelectrolyte complexation, requiring the chemical modification of LBG. Three LBG derivatives were synthesized, namely a positively charged ammonium derivative (LBGA) and negatively charged sulfate (LBGS) and carboxylate (LBGC) derivatives. These were characterized by Fourier-transform infrared spectroscopy, elemental analysis, nuclear magnetic resonance spectroscopy, gel permeation chromatography, and x-ray diffraction. As a pharmaceutical application was aimed, a toxicological analysis of the derivatives was performed by both MTT test and LDH release assay. Several nanoparticle formulations were produced using LBGA or chitosan (CS) as positively charged polymers, and LBGC or LBGS as negatively charged counterparts, producing nanoparticles with adequate properties regarding an application in oral immunization.
id RCAP_94430d319258f002e407631330b019f7
oai_identifier_str oai:sapientia.ualg.pt:10400.1/10376
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticlesCarboxylated locust bean gumPolyelectrolyte complexationPolymeric nanoparticlesSulfated locust bean gumTetraalkylammonium locust bean gumThe development of LBG-based nanoparticles intending an application in oral immunization is presented. Nanoparticle production occurred by mild polyelectrolyte complexation, requiring the chemical modification of LBG. Three LBG derivatives were synthesized, namely a positively charged ammonium derivative (LBGA) and negatively charged sulfate (LBGS) and carboxylate (LBGC) derivatives. These were characterized by Fourier-transform infrared spectroscopy, elemental analysis, nuclear magnetic resonance spectroscopy, gel permeation chromatography, and x-ray diffraction. As a pharmaceutical application was aimed, a toxicological analysis of the derivatives was performed by both MTT test and LDH release assay. Several nanoparticle formulations were produced using LBGA or chitosan (CS) as positively charged polymers, and LBGC or LBGS as negatively charged counterparts, producing nanoparticles with adequate properties regarding an application in oral immunization.SapientiaBraz, LuisGrenha, AnaCorvo, Marta C.Lourenço, João P.Ferreira, DomingosSarmento, BrunoCosta, Ana M. Rosa da2019-02-01T01:30:22Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/10376eng0144-8617AUT: LVB02213; AMG02212; JLO01215; AMC01695;10.1016/j.carbpol.2017.11.052info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:22:00Zoai:sapientia.ualg.pt:10400.1/10376Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:02:05.984279Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticles
title Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticles
spellingShingle Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticles
Braz, Luis
Carboxylated locust bean gum
Polyelectrolyte complexation
Polymeric nanoparticles
Sulfated locust bean gum
Tetraalkylammonium locust bean gum
title_short Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticles
title_full Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticles
title_fullStr Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticles
title_full_unstemmed Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticles
title_sort Synthesis and characterization of Locust Bean Gum derivatives and their application in the production of nanoparticles
author Braz, Luis
author_facet Braz, Luis
Grenha, Ana
Corvo, Marta C.
Lourenço, João P.
Ferreira, Domingos
Sarmento, Bruno
Costa, Ana M. Rosa da
author_role author
author2 Grenha, Ana
Corvo, Marta C.
Lourenço, João P.
Ferreira, Domingos
Sarmento, Bruno
Costa, Ana M. Rosa da
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Braz, Luis
Grenha, Ana
Corvo, Marta C.
Lourenço, João P.
Ferreira, Domingos
Sarmento, Bruno
Costa, Ana M. Rosa da
dc.subject.por.fl_str_mv Carboxylated locust bean gum
Polyelectrolyte complexation
Polymeric nanoparticles
Sulfated locust bean gum
Tetraalkylammonium locust bean gum
topic Carboxylated locust bean gum
Polyelectrolyte complexation
Polymeric nanoparticles
Sulfated locust bean gum
Tetraalkylammonium locust bean gum
description The development of LBG-based nanoparticles intending an application in oral immunization is presented. Nanoparticle production occurred by mild polyelectrolyte complexation, requiring the chemical modification of LBG. Three LBG derivatives were synthesized, namely a positively charged ammonium derivative (LBGA) and negatively charged sulfate (LBGS) and carboxylate (LBGC) derivatives. These were characterized by Fourier-transform infrared spectroscopy, elemental analysis, nuclear magnetic resonance spectroscopy, gel permeation chromatography, and x-ray diffraction. As a pharmaceutical application was aimed, a toxicological analysis of the derivatives was performed by both MTT test and LDH release assay. Several nanoparticle formulations were produced using LBGA or chitosan (CS) as positively charged polymers, and LBGC or LBGS as negatively charged counterparts, producing nanoparticles with adequate properties regarding an application in oral immunization.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
2019-02-01T01:30:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/10376
url http://hdl.handle.net/10400.1/10376
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0144-8617
AUT: LVB02213; AMG02212; JLO01215; AMC01695;
10.1016/j.carbpol.2017.11.052
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799133252326260736

Registros relacionados