Ocular injectable formulation assessment for oxidized dextranbased hydrogels
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10314/2720 |
Resumo: | Initiator-free injectable hydrogels are very interesting for drug and/or cell delivery applications, since they can be administered in a minimally invasive way, and avoid the use of potentially harmful chemical initiators. In the current work, oxidized dextran crosslinked with adipic acid dihydrazide hydrogels were further characterized and tuned to produce formulations, with the aim of producing an injectable formulation for the possible treatment of posterior eye diseases. The gelation rate and the hydrogel dissolution profile were shown to be dependent on the balance between the degree of dextran oxidation, and the concentration of both components. For the in vitro studies, rabbit corneal endothelial cells were seeded on the hydrogels to assess cytotoxicity. Hydrogels prepared with low oxidized dextrans were able to promote cell adhesion and proliferation to confluence in just 24 h, while more highly oxidized samples promoted cell adhesion and proliferation, but without achieving confluence. Cell viability studies were performed using MTS assays to verify the non-cytotoxicity of hydrogels and their degradation byproducts, rendering these formulations attractive for further in vivo studies |
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Ocular injectable formulation assessment for oxidized dextranbased hydrogelsHydrogelOxidized dextranIn vitroOcular deliveryInitiator-free injectable hydrogels are very interesting for drug and/or cell delivery applications, since they can be administered in a minimally invasive way, and avoid the use of potentially harmful chemical initiators. In the current work, oxidized dextran crosslinked with adipic acid dihydrazide hydrogels were further characterized and tuned to produce formulations, with the aim of producing an injectable formulation for the possible treatment of posterior eye diseases. The gelation rate and the hydrogel dissolution profile were shown to be dependent on the balance between the degree of dextran oxidation, and the concentration of both components. For the in vitro studies, rabbit corneal endothelial cells were seeded on the hydrogels to assess cytotoxicity. Hydrogels prepared with low oxidized dextrans were able to promote cell adhesion and proliferation to confluence in just 24 h, while more highly oxidized samples promoted cell adhesion and proliferation, but without achieving confluence. Cell viability studies were performed using MTS assays to verify the non-cytotoxicity of hydrogels and their degradation byproducts, rendering these formulations attractive for further in vivo studiesElsevier Ltd2016-09-14T18:36:04Z2016-09-142009-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10314/2720http://hdl.handle.net/10314/2720eng1948–1955doi:10.1016/j.actbio.2009.02.008Maia, JoãoRibeiro, MaximianoVentura, CarlaCarvalho, Rui A.Correia, Ilídio J.Gil, Maria H.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-14T02:55:44Zoai:bdigital.ipg.pt:10314/2720Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:42:13.167283Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ocular injectable formulation assessment for oxidized dextranbased hydrogels |
title |
Ocular injectable formulation assessment for oxidized dextranbased hydrogels |
spellingShingle |
Ocular injectable formulation assessment for oxidized dextranbased hydrogels Maia, João Hydrogel Oxidized dextran In vitro Ocular delivery |
title_short |
Ocular injectable formulation assessment for oxidized dextranbased hydrogels |
title_full |
Ocular injectable formulation assessment for oxidized dextranbased hydrogels |
title_fullStr |
Ocular injectable formulation assessment for oxidized dextranbased hydrogels |
title_full_unstemmed |
Ocular injectable formulation assessment for oxidized dextranbased hydrogels |
title_sort |
Ocular injectable formulation assessment for oxidized dextranbased hydrogels |
author |
Maia, João |
author_facet |
Maia, João Ribeiro, Maximiano Ventura, Carla Carvalho, Rui A. Correia, Ilídio J. Gil, Maria H. |
author_role |
author |
author2 |
Ribeiro, Maximiano Ventura, Carla Carvalho, Rui A. Correia, Ilídio J. Gil, Maria H. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Maia, João Ribeiro, Maximiano Ventura, Carla Carvalho, Rui A. Correia, Ilídio J. Gil, Maria H. |
dc.subject.por.fl_str_mv |
Hydrogel Oxidized dextran In vitro Ocular delivery |
topic |
Hydrogel Oxidized dextran In vitro Ocular delivery |
description |
Initiator-free injectable hydrogels are very interesting for drug and/or cell delivery applications, since they can be administered in a minimally invasive way, and avoid the use of potentially harmful chemical initiators. In the current work, oxidized dextran crosslinked with adipic acid dihydrazide hydrogels were further characterized and tuned to produce formulations, with the aim of producing an injectable formulation for the possible treatment of posterior eye diseases. The gelation rate and the hydrogel dissolution profile were shown to be dependent on the balance between the degree of dextran oxidation, and the concentration of both components. For the in vitro studies, rabbit corneal endothelial cells were seeded on the hydrogels to assess cytotoxicity. Hydrogels prepared with low oxidized dextrans were able to promote cell adhesion and proliferation to confluence in just 24 h, while more highly oxidized samples promoted cell adhesion and proliferation, but without achieving confluence. Cell viability studies were performed using MTS assays to verify the non-cytotoxicity of hydrogels and their degradation byproducts, rendering these formulations attractive for further in vivo studies |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-07-01T00:00:00Z 2016-09-14T18:36:04Z 2016-09-14 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10314/2720 http://hdl.handle.net/10314/2720 |
url |
http://hdl.handle.net/10314/2720 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1948–1955 doi:10.1016/j.actbio.2009.02.008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier Ltd |
publisher.none.fl_str_mv |
Elsevier Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136914639421440 |