Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollen
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/22345 |
Resumo: | The aim of this study was to evaluate the influence of lyophilization and drying in stove on phenolic compounds content and the biological activity of Apis mellifera and Trigona spinipes pollens produced in Brazil. In general, the bee pollen produced by T. spinipes presented highest antioxidant activity in dried and fresh samples assessed either by test of plasma ferric reduction capacity (FRAP) and free radical scavenging assay (DPPH) methods. For A. mellifera bee pollen the antioxidant activity was higher on the fresh samples. Nevertheless, b-carotene bleaching assay (BCB) and linoleic acid content were higher in T. spinipes samples, mainly in the fresh ones. Higher antioxidant activity was owing to higher content in phenolic compounds. Lyophilization method was the best for phenolic compounds’ conservation for both species. The bee pollen of both species has a high amount of flavonoids: kaempferol-3-O-glucoside was the most abundant in A. mellifera while for T. spinipes the most prevalent was resorcylic acidþepicatechin. All extracts presented antibacterial activity against Saphylococcus aureus (ATCC 43300)TM, (ESA 83138150), (ESA 32), Pseudomonas aeruginosa (ATCC 15442)TM, Pseudomonas aeruginosa (MRC.4) and (MRC.10). The methods used for storage influenced the biological properties of bee pollen from both species. Regarding the content of phenolic compounds, differences were observed amongst the pollen types: for A. mellifera these were best preserved with lyophilization, while for T. spinipes the three storage methods were equivalent. |
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Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollenHoneyHPLC-PDAPseudomonas aeruginosaPseudomonas sppStaphylococcus aureusThe aim of this study was to evaluate the influence of lyophilization and drying in stove on phenolic compounds content and the biological activity of Apis mellifera and Trigona spinipes pollens produced in Brazil. In general, the bee pollen produced by T. spinipes presented highest antioxidant activity in dried and fresh samples assessed either by test of plasma ferric reduction capacity (FRAP) and free radical scavenging assay (DPPH) methods. For A. mellifera bee pollen the antioxidant activity was higher on the fresh samples. Nevertheless, b-carotene bleaching assay (BCB) and linoleic acid content were higher in T. spinipes samples, mainly in the fresh ones. Higher antioxidant activity was owing to higher content in phenolic compounds. Lyophilization method was the best for phenolic compounds’ conservation for both species. The bee pollen of both species has a high amount of flavonoids: kaempferol-3-O-glucoside was the most abundant in A. mellifera while for T. spinipes the most prevalent was resorcylic acidþepicatechin. All extracts presented antibacterial activity against Saphylococcus aureus (ATCC 43300)TM, (ESA 83138150), (ESA 32), Pseudomonas aeruginosa (ATCC 15442)TM, Pseudomonas aeruginosa (MRC.4) and (MRC.10). The methods used for storage influenced the biological properties of bee pollen from both species. Regarding the content of phenolic compounds, differences were observed amongst the pollen types: for A. mellifera these were best preserved with lyophilization, while for T. spinipes the three storage methods were equivalent.The authors are grateful to the Coordenac¸~ao de Aperfeic¸oamento de Pessoal de N ıvel Superior – CAPES (88881.062167/2014-01 for M.L.M.E.). A. Pascoal would like to thank Foundation for Science and Technology (FCT, Portugal), Programa Operacional Potencial Humano (POPH), and European Union (EU) for his Postdoctoral grant SFRH/BPD/ 91380/2012.Biblioteca Digital do IPBSanta Bárbara, Marivalda F.Moreira, Manuela M.Machado, Cerilene SantiagoChambó, Emerson DechechiPascoal, AnaniasCarvalho, Carlos Alfredo Lopes deSodré, Geni da SilvaDelerue-Matos, CristinaEstevinho, Leticia M.2018-01-19T10:00:00Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/22345engSanta Bárbara, Marivalda F.; Moreira, Manuela M.; Machado, Cerilene S.; Chambó, Emerson D.; Pascoal, Ananias; de Carvalho, Carlos Alfredo L.; da Silva Sodré, Geni; Delerue-Matos, Cristina; Estevinho, Leticia M. (2020). Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollen. Journal of Apicultural Research. ISSN 0021-8839. p. 1-100021-883910.1080/00218839.2019.1708595info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:50:06Zoai:bibliotecadigital.ipb.pt:10198/22345Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:13:35.632202Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollen |
title |
Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollen |
spellingShingle |
Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollen Santa Bárbara, Marivalda F. Honey HPLC-PDA Pseudomonas aeruginosa Pseudomonas spp Staphylococcus aureus |
title_short |
Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollen |
title_full |
Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollen |
title_fullStr |
Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollen |
title_full_unstemmed |
Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollen |
title_sort |
Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollen |
author |
Santa Bárbara, Marivalda F. |
author_facet |
Santa Bárbara, Marivalda F. Moreira, Manuela M. Machado, Cerilene Santiago Chambó, Emerson Dechechi Pascoal, Ananias Carvalho, Carlos Alfredo Lopes de Sodré, Geni da Silva Delerue-Matos, Cristina Estevinho, Leticia M. |
author_role |
author |
author2 |
Moreira, Manuela M. Machado, Cerilene Santiago Chambó, Emerson Dechechi Pascoal, Ananias Carvalho, Carlos Alfredo Lopes de Sodré, Geni da Silva Delerue-Matos, Cristina Estevinho, Leticia M. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Santa Bárbara, Marivalda F. Moreira, Manuela M. Machado, Cerilene Santiago Chambó, Emerson Dechechi Pascoal, Ananias Carvalho, Carlos Alfredo Lopes de Sodré, Geni da Silva Delerue-Matos, Cristina Estevinho, Leticia M. |
dc.subject.por.fl_str_mv |
Honey HPLC-PDA Pseudomonas aeruginosa Pseudomonas spp Staphylococcus aureus |
topic |
Honey HPLC-PDA Pseudomonas aeruginosa Pseudomonas spp Staphylococcus aureus |
description |
The aim of this study was to evaluate the influence of lyophilization and drying in stove on phenolic compounds content and the biological activity of Apis mellifera and Trigona spinipes pollens produced in Brazil. In general, the bee pollen produced by T. spinipes presented highest antioxidant activity in dried and fresh samples assessed either by test of plasma ferric reduction capacity (FRAP) and free radical scavenging assay (DPPH) methods. For A. mellifera bee pollen the antioxidant activity was higher on the fresh samples. Nevertheless, b-carotene bleaching assay (BCB) and linoleic acid content were higher in T. spinipes samples, mainly in the fresh ones. Higher antioxidant activity was owing to higher content in phenolic compounds. Lyophilization method was the best for phenolic compounds’ conservation for both species. The bee pollen of both species has a high amount of flavonoids: kaempferol-3-O-glucoside was the most abundant in A. mellifera while for T. spinipes the most prevalent was resorcylic acidþepicatechin. All extracts presented antibacterial activity against Saphylococcus aureus (ATCC 43300)TM, (ESA 83138150), (ESA 32), Pseudomonas aeruginosa (ATCC 15442)TM, Pseudomonas aeruginosa (MRC.4) and (MRC.10). The methods used for storage influenced the biological properties of bee pollen from both species. Regarding the content of phenolic compounds, differences were observed amongst the pollen types: for A. mellifera these were best preserved with lyophilization, while for T. spinipes the three storage methods were equivalent. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/22345 |
url |
http://hdl.handle.net/10198/22345 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santa Bárbara, Marivalda F.; Moreira, Manuela M.; Machado, Cerilene S.; Chambó, Emerson D.; Pascoal, Ananias; de Carvalho, Carlos Alfredo L.; da Silva Sodré, Geni; Delerue-Matos, Cristina; Estevinho, Leticia M. (2020). Storage methods, phenolic composition, and bioactive properties of Apis mellifera and Trigona spinipes pollen. Journal of Apicultural Research. ISSN 0021-8839. p. 1-10 0021-8839 10.1080/00218839.2019.1708595 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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