Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/23782 |
Resumo: | Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated on apple pulp, a glucose- and fructoserich waste generated during juice production, to produce medium-chain length polyhydroxyalkanoates. A cell dry mass of 8.74 ± 0.20 g/L, with a polymer content of 49.25 ± 4.08% were attained. The produced biopolymer was composed of 42.7±0.1mol% 3-hydroxydecanoate, 17.9±1.0mol% 3-hydroxyoctanoate, 14.5±1.1mol% 3- hydroxybutyrate, 11.1±0.6 mol% 3-hydroxytetradecanoate, 10.1±0.5 mol% 3-hydroxydodecanoate and 3.7± 0.2 mol% 3-hydroxyhexanoate. It presented low glass transition and melting temperatures (−40.9 ± 0.7 °C and 42.0±0.1 °C, respectively), and a degradation temperature of 300.0±0.1 °C, coupled to a lowcrystallinity index (12.7±2.7%), amolecularweight (Mw) of 1.34 × 105±0.18×105 Da and a polydispersity index of 2.70±0.03. The biopolymer's filmswere dense and had a smooth surface, as demonstrated by Scanning ElectronMicroscopy. They presented a tension at break of 5.21 ± 1.09 MPa, together with an elongation of 400.5 ± 55.8% and an associated Young modulus of 4.86 ± 1.49 MPa, under tensile tests. These attractive filming properties of this biopolymer could potentially be valorised in several areas such as the fine chemicals industry, biomedicine, pharmaceuticals, or food packaging |
id |
RCAP_95aa5ffbdc777f65910804a99a68cc0f |
---|---|
oai_identifier_str |
oai:www.repository.utl.pt:10400.5/23782 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterizationfruit pulp wastepolyhydroxyalkanoates (PHA)Pseudomonas chlororaphisPseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated on apple pulp, a glucose- and fructoserich waste generated during juice production, to produce medium-chain length polyhydroxyalkanoates. A cell dry mass of 8.74 ± 0.20 g/L, with a polymer content of 49.25 ± 4.08% were attained. The produced biopolymer was composed of 42.7±0.1mol% 3-hydroxydecanoate, 17.9±1.0mol% 3-hydroxyoctanoate, 14.5±1.1mol% 3- hydroxybutyrate, 11.1±0.6 mol% 3-hydroxytetradecanoate, 10.1±0.5 mol% 3-hydroxydodecanoate and 3.7± 0.2 mol% 3-hydroxyhexanoate. It presented low glass transition and melting temperatures (−40.9 ± 0.7 °C and 42.0±0.1 °C, respectively), and a degradation temperature of 300.0±0.1 °C, coupled to a lowcrystallinity index (12.7±2.7%), amolecularweight (Mw) of 1.34 × 105±0.18×105 Da and a polydispersity index of 2.70±0.03. The biopolymer's filmswere dense and had a smooth surface, as demonstrated by Scanning ElectronMicroscopy. They presented a tension at break of 5.21 ± 1.09 MPa, together with an elongation of 400.5 ± 55.8% and an associated Young modulus of 4.86 ± 1.49 MPa, under tensile tests. These attractive filming properties of this biopolymer could potentially be valorised in several areas such as the fine chemicals industry, biomedicine, pharmaceuticals, or food packagingElsevierRepositório da Universidade de LisboaPereira, João R.Araújo, DianaFreitas, PatríciaMarques, Ana C.Delgado Alves, VitorSevrin, ChantalGrandfils, ChristianFortunato, ElviraReis, Maria A.M.Freitas, Filomena2023-01-01T01:30:24Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/23782engPereira JR, Araújo D, Freitas P, Marques AC, Alves VD, Sevrin C, Grandfils C, Fortunato E, Reis MAM, Freitas F (2021). Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization. International Journal of Biological Macromolecules, 167:85-92https://doi.org/10.1016/j.ijbiomac.2020.11.162info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:24Zoai:www.repository.utl.pt:10400.5/23782Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:07:55.723042Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization |
title |
Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization |
spellingShingle |
Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization Pereira, João R. fruit pulp waste polyhydroxyalkanoates (PHA) Pseudomonas chlororaphis |
title_short |
Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization |
title_full |
Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization |
title_fullStr |
Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization |
title_full_unstemmed |
Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization |
title_sort |
Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization |
author |
Pereira, João R. |
author_facet |
Pereira, João R. Araújo, Diana Freitas, Patrícia Marques, Ana C. Delgado Alves, Vitor Sevrin, Chantal Grandfils, Christian Fortunato, Elvira Reis, Maria A.M. Freitas, Filomena |
author_role |
author |
author2 |
Araújo, Diana Freitas, Patrícia Marques, Ana C. Delgado Alves, Vitor Sevrin, Chantal Grandfils, Christian Fortunato, Elvira Reis, Maria A.M. Freitas, Filomena |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Pereira, João R. Araújo, Diana Freitas, Patrícia Marques, Ana C. Delgado Alves, Vitor Sevrin, Chantal Grandfils, Christian Fortunato, Elvira Reis, Maria A.M. Freitas, Filomena |
dc.subject.por.fl_str_mv |
fruit pulp waste polyhydroxyalkanoates (PHA) Pseudomonas chlororaphis |
topic |
fruit pulp waste polyhydroxyalkanoates (PHA) Pseudomonas chlororaphis |
description |
Pseudomonas chlororaphis subsp. aurantiaca DSM 19603 was cultivated on apple pulp, a glucose- and fructoserich waste generated during juice production, to produce medium-chain length polyhydroxyalkanoates. A cell dry mass of 8.74 ± 0.20 g/L, with a polymer content of 49.25 ± 4.08% were attained. The produced biopolymer was composed of 42.7±0.1mol% 3-hydroxydecanoate, 17.9±1.0mol% 3-hydroxyoctanoate, 14.5±1.1mol% 3- hydroxybutyrate, 11.1±0.6 mol% 3-hydroxytetradecanoate, 10.1±0.5 mol% 3-hydroxydodecanoate and 3.7± 0.2 mol% 3-hydroxyhexanoate. It presented low glass transition and melting temperatures (−40.9 ± 0.7 °C and 42.0±0.1 °C, respectively), and a degradation temperature of 300.0±0.1 °C, coupled to a lowcrystallinity index (12.7±2.7%), amolecularweight (Mw) of 1.34 × 105±0.18×105 Da and a polydispersity index of 2.70±0.03. The biopolymer's filmswere dense and had a smooth surface, as demonstrated by Scanning ElectronMicroscopy. They presented a tension at break of 5.21 ± 1.09 MPa, together with an elongation of 400.5 ± 55.8% and an associated Young modulus of 4.86 ± 1.49 MPa, under tensile tests. These attractive filming properties of this biopolymer could potentially be valorised in several areas such as the fine chemicals industry, biomedicine, pharmaceuticals, or food packaging |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2023-01-01T01:30:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/23782 |
url |
http://hdl.handle.net/10400.5/23782 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pereira JR, Araújo D, Freitas P, Marques AC, Alves VD, Sevrin C, Grandfils C, Fortunato E, Reis MAM, Freitas F (2021). Production of medium-chain-length polyhydroxyalkanoates by Pseudomonas chlororaphis subsp. aurantiaca: Cultivation on fruit pulp waste and polymer characterization. International Journal of Biological Macromolecules, 167:85-92 https://doi.org/10.1016/j.ijbiomac.2020.11.162 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131173503369216 |