Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1371/journal.pone.0127536 |
Resumo: | PMID:26017968 WOS:000355185600088 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West AfricaBiochemistry, Genetics and Molecular Biology (miscellaneous)Infectious DiseasesEpidemiologySDG 3 - Good Health and Well-beingSDG 10 - Reduced InequalitiesPMID:26017968 WOS:000355185600088Background This study aimed to evaluate the usefulness of the Xpert MTB/RIF assay for the rapid direct detection of M. tuberculosis complex (MTBC) strains and rifampicin resistance associated mutations in a resource-limited setting such as Guinea-Bissau and its implications in the management of tuberculosis (TB) and drug resistant tuberculosis, complementing the scarce information on resistance and genotypic diversity of MTBC strains in this West African country. Methods and Results This cross-sectional prospective study included 100 consecutive TB patients with positive acid-fast smears at two months of anti-tuberculosis treatment or in a re-treatment situation, between May and December 2012. Resistance to rifampicin was detected using the GeneXpert system and the Xpert MTB/RIF assay. MTBC isolates obtained with the BACTEC MGIT 960 system were tested for susceptibility to first- And second-line anti-tuberculosis drugs. Overall, the prevalence of multidrug-resistant tuberculosis (MDR-TB) was found to be 9 cases. Of these, 67% (6 patients) of confirmed MDR-TB cases had no past history of TB treatment and 33% (3 patients) were previously treated cases. Extensively drug-resistant TB was not found. Molecular typing of the MDR-TB strains revealed recent transmission patterns of imported MDR strains. Conclusions The Xpert MTB/RIF assay was reliable for the detection of rifampicin resistant MTBC strains directly from sputum samples of patients undergoing first-line treatment for two months, being more trustworthy than the simple presence of acid-fast bacilli in the smear. Its implementation is technically simple, does not require specialized laboratory infrastructures and is suitable for resource-limited settings when a regular source of electricity and maintenance is available as well as financial and operation sustainability is guaranteed by the health authorities. A high prevalence of MDR-TB among patients at risk of MDR-TB after two months of first-line treatment was found, in support of the WHO recommendations for its use in the management of this risk group.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)TB, HIV and opportunistic diseases and pathogens (THOP)Vector borne diseases and pathogens (VBD)RUNRabna, PauloRamos, JorgePonce, GemaSanca, LilicaMané, MortoArmada, AnaMachado, DianaVieira, FinaGomes, Victor F.Martins, ElisabeteColombatti, RaffaellaRiccardi, FabioPerdigão, JoãoSotero, JoanaPortugal, IsabelCouto, IsabelAtouguia, JorgeRodrigues, AmabéliaViveiros, Miguel2018-05-14T22:02:18Z2015-05-012015-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1371/journal.pone.0127536eng1932-6203PURE: 1731242http://www.scopus.com/inward/record.url?scp=84958198245&partnerID=8YFLogxKhttps://doi.org/10.1371/journal.pone.0127536info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:30Zoai:run.unl.pt:10362/36943Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:43.119612Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa |
title |
Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa |
spellingShingle |
Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa Rabna, Paulo Biochemistry, Genetics and Molecular Biology (miscellaneous) Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being SDG 10 - Reduced Inequalities |
title_short |
Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa |
title_full |
Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa |
title_fullStr |
Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa |
title_full_unstemmed |
Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa |
title_sort |
Direct detection by the xpert MTB/RIF assay and characterization of multi and poly drug- resistant tuberculosis in Guinea-Bissau, West Africa |
author |
Rabna, Paulo |
author_facet |
Rabna, Paulo Ramos, Jorge Ponce, Gema Sanca, Lilica Mané, Morto Armada, Ana Machado, Diana Vieira, Fina Gomes, Victor F. Martins, Elisabete Colombatti, Raffaella Riccardi, Fabio Perdigão, João Sotero, Joana Portugal, Isabel Couto, Isabel Atouguia, Jorge Rodrigues, Amabélia Viveiros, Miguel |
author_role |
author |
author2 |
Ramos, Jorge Ponce, Gema Sanca, Lilica Mané, Morto Armada, Ana Machado, Diana Vieira, Fina Gomes, Victor F. Martins, Elisabete Colombatti, Raffaella Riccardi, Fabio Perdigão, João Sotero, Joana Portugal, Isabel Couto, Isabel Atouguia, Jorge Rodrigues, Amabélia Viveiros, Miguel |
author2_role |
author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) TB, HIV and opportunistic diseases and pathogens (THOP) Vector borne diseases and pathogens (VBD) RUN |
dc.contributor.author.fl_str_mv |
Rabna, Paulo Ramos, Jorge Ponce, Gema Sanca, Lilica Mané, Morto Armada, Ana Machado, Diana Vieira, Fina Gomes, Victor F. Martins, Elisabete Colombatti, Raffaella Riccardi, Fabio Perdigão, João Sotero, Joana Portugal, Isabel Couto, Isabel Atouguia, Jorge Rodrigues, Amabélia Viveiros, Miguel |
dc.subject.por.fl_str_mv |
Biochemistry, Genetics and Molecular Biology (miscellaneous) Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being SDG 10 - Reduced Inequalities |
topic |
Biochemistry, Genetics and Molecular Biology (miscellaneous) Infectious Diseases Epidemiology SDG 3 - Good Health and Well-being SDG 10 - Reduced Inequalities |
description |
PMID:26017968 WOS:000355185600088 |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-05-01 2015-05-01T00:00:00Z 2018-05-14T22:02:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1371/journal.pone.0127536 |
url |
https://doi.org/10.1371/journal.pone.0127536 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 PURE: 1731242 http://www.scopus.com/inward/record.url?scp=84958198245&partnerID=8YFLogxK https://doi.org/10.1371/journal.pone.0127536 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137931375411200 |