Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/86020 |
Resumo: | Regeneration of severe bone defects remains a challenge. A formulation of synthetic glass-reinforced hydroxyapatite bone substitute, Bonelike® Poro (BL®P), 250500 µm-diameter, with a dextrin-based hydrogel (HG), further loaded with human dental pulp stem cells (hDPSCs) with osteogenic differentiation ability, was tested for the management of critical-sized defects in an ovine model. Morphology, calcium release, and mechanical strength of HG + BL®P were analyzed. Then, BL®P, HG + BL®P, and 106 hDPSCs-loaded HG + BL®P were implanted in ovine critical-sized 14 mm-diameter calvaria defects. Bone samples were collected after 3 and 6 weeks for histological and micro-CT analysis. BL®P exhibits a suitable porous size for cell ingrowth, from the nm (>200 nm) to the µm (5 µm) range. The addition of BL®P granules to the HG resulted in increased compressive elastic modulus and ultimate tensile strength. The mildly acidic nature of the HG contributed to a faster dissolution of granules. In vivo results confirmed the HG suitability as a carrier, providing better defect filling, easy handling, and injectability of BL®P without compromising new bone formation nor biocompatibility. The HG + BL®P formulations can successfully regenerate critical-sized defects; however, addition of hDPSCs did not significantly enhance new bone formation under these conditions. Granular BL®P provides an effective alternative to autologous grafts. The HG acts as a biocompatible carrier of granular bone substitutes and cells, conferring injectability and cohesivity. |
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Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repairDextrinInjectableHydrogelBone regenerationStem cellsDental pulpRegeneration of severe bone defects remains a challenge. A formulation of synthetic glass-reinforced hydroxyapatite bone substitute, Bonelike® Poro (BL®P), 250500 µm-diameter, with a dextrin-based hydrogel (HG), further loaded with human dental pulp stem cells (hDPSCs) with osteogenic differentiation ability, was tested for the management of critical-sized defects in an ovine model. Morphology, calcium release, and mechanical strength of HG + BL®P were analyzed. Then, BL®P, HG + BL®P, and 106 hDPSCs-loaded HG + BL®P were implanted in ovine critical-sized 14 mm-diameter calvaria defects. Bone samples were collected after 3 and 6 weeks for histological and micro-CT analysis. BL®P exhibits a suitable porous size for cell ingrowth, from the nm (>200 nm) to the µm (5 µm) range. The addition of BL®P granules to the HG resulted in increased compressive elastic modulus and ultimate tensile strength. The mildly acidic nature of the HG contributed to a faster dissolution of granules. In vivo results confirmed the HG suitability as a carrier, providing better defect filling, easy handling, and injectability of BL®P without compromising new bone formation nor biocompatibility. The HG + BL®P formulations can successfully regenerate critical-sized defects; however, addition of hDPSCs did not significantly enhance new bone formation under these conditions. Granular BL®P provides an effective alternative to autologous grafts. The HG acts as a biocompatible carrier of granular bone substitutes and cells, conferring injectability and cohesivity.Alexandra Machado and Isabel Pereira were supported by the grants SFRH/BD/132000/2017 and UMINHO/BI/131/2018 respectively, from Portuguese Foundation for Science and Technology (FCT), Portugal. The authors acknowledge the funding from FEDER and NORTE 2020 through the project no. 003262 titled “iBONE therapies: advanced solutions for bone regeneration”. This study was supported by FCT under the scope of the strategic funding of UID/BIO/04469 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte 2020 - Programa Operacional Regional do Norte. The participation of Isabel Pires, Justina Prada, Luís Maltez and José Eduardo Pereira was funded by the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 supported by FCT. The participation of Rui Alvites, Ana Catarina Sousa, Mariana Branquinho, Ana Rita Caseiro, Sílvia Santos Pedrosa and Ana Colette Maurício was funded by Projects PEst-OE/AGR/UI0211/2011, UIDB/CVT/00772/2020 and LA/P/0059/2020. Mariana Vieira Branquinho (SFRH/BD/146172/2019) and Ana Catarina Sousa (SFRH/BD/146689/2019) acknowledge FCT, for financial support.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoMachado, A.Pereira, IsabelPereira, José EduardoMaltez, LuísBrandão, AnaAlvites, RuiSousa, Ana CatarinaBranquinho, MarianaCaseiro, Ana RitaPedrosa, Sílvia SantosMaurício, Ana ColettePires, IsabelPrada, JustinaSantos, José DomingosGama, F. M.2023-07-292023-07-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/86020engMachado, A., Pereira, I., Pereira, J. E., Maltez, L., Brandão, A., Alvites, R., … Gama, M. (2023, August). Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair. Materialia. Elsevier BV. http://doi.org/10.1016/j.mtla.2023.1018592589152910.1016/j.mtla.2023.101859https://www.sciencedirect.com/science/article/pii/S2589152923001862info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-12T01:18:19Zoai:repositorium.sdum.uminho.pt:1822/86020Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:26:40.434868Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair |
title |
Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair |
spellingShingle |
Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair Machado, A. Dextrin Injectable Hydrogel Bone regeneration Stem cells Dental pulp |
title_short |
Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair |
title_full |
Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair |
title_fullStr |
Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair |
title_full_unstemmed |
Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair |
title_sort |
Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair |
author |
Machado, A. |
author_facet |
Machado, A. Pereira, Isabel Pereira, José Eduardo Maltez, Luís Brandão, Ana Alvites, Rui Sousa, Ana Catarina Branquinho, Mariana Caseiro, Ana Rita Pedrosa, Sílvia Santos Maurício, Ana Colette Pires, Isabel Prada, Justina Santos, José Domingos Gama, F. M. |
author_role |
author |
author2 |
Pereira, Isabel Pereira, José Eduardo Maltez, Luís Brandão, Ana Alvites, Rui Sousa, Ana Catarina Branquinho, Mariana Caseiro, Ana Rita Pedrosa, Sílvia Santos Maurício, Ana Colette Pires, Isabel Prada, Justina Santos, José Domingos Gama, F. M. |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Machado, A. Pereira, Isabel Pereira, José Eduardo Maltez, Luís Brandão, Ana Alvites, Rui Sousa, Ana Catarina Branquinho, Mariana Caseiro, Ana Rita Pedrosa, Sílvia Santos Maurício, Ana Colette Pires, Isabel Prada, Justina Santos, José Domingos Gama, F. M. |
dc.subject.por.fl_str_mv |
Dextrin Injectable Hydrogel Bone regeneration Stem cells Dental pulp |
topic |
Dextrin Injectable Hydrogel Bone regeneration Stem cells Dental pulp |
description |
Regeneration of severe bone defects remains a challenge. A formulation of synthetic glass-reinforced hydroxyapatite bone substitute, Bonelike® Poro (BL®P), 250500 µm-diameter, with a dextrin-based hydrogel (HG), further loaded with human dental pulp stem cells (hDPSCs) with osteogenic differentiation ability, was tested for the management of critical-sized defects in an ovine model. Morphology, calcium release, and mechanical strength of HG + BL®P were analyzed. Then, BL®P, HG + BL®P, and 106 hDPSCs-loaded HG + BL®P were implanted in ovine critical-sized 14 mm-diameter calvaria defects. Bone samples were collected after 3 and 6 weeks for histological and micro-CT analysis. BL®P exhibits a suitable porous size for cell ingrowth, from the nm (>200 nm) to the µm (5 µm) range. The addition of BL®P granules to the HG resulted in increased compressive elastic modulus and ultimate tensile strength. The mildly acidic nature of the HG contributed to a faster dissolution of granules. In vivo results confirmed the HG suitability as a carrier, providing better defect filling, easy handling, and injectability of BL®P without compromising new bone formation nor biocompatibility. The HG + BL®P formulations can successfully regenerate critical-sized defects; however, addition of hDPSCs did not significantly enhance new bone formation under these conditions. Granular BL®P provides an effective alternative to autologous grafts. The HG acts as a biocompatible carrier of granular bone substitutes and cells, conferring injectability and cohesivity. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29 2023-07-29T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/86020 |
url |
https://hdl.handle.net/1822/86020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Machado, A., Pereira, I., Pereira, J. E., Maltez, L., Brandão, A., Alvites, R., … Gama, M. (2023, August). Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair. Materialia. Elsevier BV. http://doi.org/10.1016/j.mtla.2023.101859 25891529 10.1016/j.mtla.2023.101859 https://www.sciencedirect.com/science/article/pii/S2589152923001862 |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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