In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system

Detalhes bibliográficos
Autor(a) principal: Afonso, T. B.
Data de Publicação: 2019
Outros Autores: Simões, Lúcia C., Lima, Nelson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/63345
Resumo: The main focus so far in the study of biofilm formation in drinking water has been bacteria. Studies on biofilm formation involving filamentous fungi are, therefore, scarce. This study aimed to assess and characterize the ability of these microorganisms to interact with bacteria whilst forming inter-kingdom biofilms. Biofilms were analysed in terms of total biomass, metabolic activity, bacterial colony forming units and morphology by epifluorescence microscopy. The quantitative methods revealed that biofilm mass increased over time for both single and inter-kingdom biofilms, while specific metabolic activity decreased, in general, along the time points evaluated. Microscopic data visually confirmed the biofilm mass increase over time. This study shows that fungal stage development is important in the first 24?h of biofilm formation. Inter-kingdom biofilm formation is microorganism dependent and inter-kingdom biofilms may provide an advantage to the opportunistic bacterium Acinetobacter calcoaceticus to replicate and proliferate when compared with Methylobacterium oryzae.
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spelling In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution systemPenicillium expansumPenicillium brevicompactumbacteriabiomass quantificationmicrotitre platesmicroscopyPenicillium expansum; Penicillium brevicompactumScience & TechnologyThe main focus so far in the study of biofilm formation in drinking water has been bacteria. Studies on biofilm formation involving filamentous fungi are, therefore, scarce. This study aimed to assess and characterize the ability of these microorganisms to interact with bacteria whilst forming inter-kingdom biofilms. Biofilms were analysed in terms of total biomass, metabolic activity, bacterial colony forming units and morphology by epifluorescence microscopy. The quantitative methods revealed that biofilm mass increased over time for both single and inter-kingdom biofilms, while specific metabolic activity decreased, in general, along the time points evaluated. Microscopic data visually confirmed the biofilm mass increase over time. This study shows that fungal stage development is important in the first 24?h of biofilm formation. Inter-kingdom biofilm formation is microorganism dependent and inter-kingdom biofilms may provide an advantage to the opportunistic bacterium Acinetobacter calcoaceticus to replicate and proliferate when compared with Methylobacterium oryzae.Tiago B. Afonso acknowledges the grant provided by the Portuguese Foundation for Science and Technology (FCT) under grant no. PD/BD/128033/2016. This study was also supported by FCT under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Fundação para a Ciência e a Tecnologia.info:eu-repo/semantics/publishedVersionTaylor and FrancisUniversidade do MinhoAfonso, T. B.Simões, Lúcia C.Lima, Nelson2019-11-282019-11-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/63345engAfonso, T. B.; Simões, Lúcia C.; Lima, Nelson, In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system. Biofouling, 35(10), 1041-1054, 20190892-70141029-245410.1080/08927014.2019.168879331778071http://www.tandfonline.com/action/journalInformation?journalCode=gbif20info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:11:49Zoai:repositorium.sdum.uminho.pt:1822/63345Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:03:38.553917Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system
title In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system
spellingShingle In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system
Afonso, T. B.
Penicillium expansum
Penicillium brevicompactum
bacteria
biomass quantification
microtitre plates
microscopy
Penicillium expansum; Penicillium brevicompactum
Science & Technology
title_short In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system
title_full In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system
title_fullStr In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system
title_full_unstemmed In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system
title_sort In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system
author Afonso, T. B.
author_facet Afonso, T. B.
Simões, Lúcia C.
Lima, Nelson
author_role author
author2 Simões, Lúcia C.
Lima, Nelson
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Afonso, T. B.
Simões, Lúcia C.
Lima, Nelson
dc.subject.por.fl_str_mv Penicillium expansum
Penicillium brevicompactum
bacteria
biomass quantification
microtitre plates
microscopy
Penicillium expansum; Penicillium brevicompactum
Science & Technology
topic Penicillium expansum
Penicillium brevicompactum
bacteria
biomass quantification
microtitre plates
microscopy
Penicillium expansum; Penicillium brevicompactum
Science & Technology
description The main focus so far in the study of biofilm formation in drinking water has been bacteria. Studies on biofilm formation involving filamentous fungi are, therefore, scarce. This study aimed to assess and characterize the ability of these microorganisms to interact with bacteria whilst forming inter-kingdom biofilms. Biofilms were analysed in terms of total biomass, metabolic activity, bacterial colony forming units and morphology by epifluorescence microscopy. The quantitative methods revealed that biofilm mass increased over time for both single and inter-kingdom biofilms, while specific metabolic activity decreased, in general, along the time points evaluated. Microscopic data visually confirmed the biofilm mass increase over time. This study shows that fungal stage development is important in the first 24?h of biofilm formation. Inter-kingdom biofilm formation is microorganism dependent and inter-kingdom biofilms may provide an advantage to the opportunistic bacterium Acinetobacter calcoaceticus to replicate and proliferate when compared with Methylobacterium oryzae.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-28
2019-11-28T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/63345
url http://hdl.handle.net/1822/63345
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Afonso, T. B.; Simões, Lúcia C.; Lima, Nelson, In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system. Biofouling, 35(10), 1041-1054, 2019
0892-7014
1029-2454
10.1080/08927014.2019.1688793
31778071
http://www.tandfonline.com/action/journalInformation?journalCode=gbif20
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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