Direct targeting of hippocampal neurons for apoptosis by glucocorticoids is reversible by mineralocorticoid receptor activation

Detalhes bibliográficos
Autor(a) principal: Crochemore, C.
Data de Publicação: 2005
Outros Autores: Lu, J., Wu, Y., Liposits, Zs., Sousa, Nuno, Holsboer, F., Almeida, O. F. X.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/2944
Resumo: Prova tipográfica (In Press)
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spelling Direct targeting of hippocampal neurons for apoptosis by glucocorticoids is reversible by mineralocorticoid receptor activationApoptosisDexamethasoneGlucocorticoid receptorMineralocorticoid receptorSpironolactoneRU28318HippocampusScience & TechnologyProva tipográfica (In Press)An important question arising from previous observations in vivo is whether glucocorticoids can directly influence neuronal survival in the hippocampus. To this end, a primary postnatal hippocampal culture system containing mature neurons and expressing both glucocorticoid (GR) and mineralocorticoid (MR) receptors was developed. Results show that the GR agonist dexamethasone (DEX) targets neurons (microtubule-associated protein 2-positive cells) for death through apoptosis. GR-mediated cell death was counteracted by the MR agonist aldosterone (ALDO). Antagonism of MR with spironolactone ([7a-(acetylthio)-3-oxo-17a-pregn- 4-ene,21 carbolactone] (SPIRO)) causes a dose-dependent increase in neuronal apoptosis in the absence of DEX, indicating that nanomolar levels of corticosterone present in the culture medium, which are sufficient to activate MR, can mask the apoptotic response to DEX. Indeed, both SPIRO and another MR antagonist, oxprenoate potassium ((7a,17a)-17-Hydroxy-3-oxo-7- propylpregn-4-ene-21-carboxylic acid, potassium salt (RU28318)), accentuated DEX-induced apoptosis. These results demonstrate that GRs can act directly to induce hippocampal neuronal death and that demonstration of their full apoptotic potency depends on abolition of survival-promoting actions mediated by MR.Nature Publishing GroupUniversidade do MinhoCrochemore, C.Lu, J.Wu, Y.Liposits, Zs.Sousa, NunoHolsboer, F.Almeida, O. F. X.2005-09-162005-09-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/2944eng1359-418410.1038/sj.mp.400167915940303info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:11:15Zoai:repositorium.sdum.uminho.pt:1822/2944Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:03:00.284583Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Direct targeting of hippocampal neurons for apoptosis by glucocorticoids is reversible by mineralocorticoid receptor activation
title Direct targeting of hippocampal neurons for apoptosis by glucocorticoids is reversible by mineralocorticoid receptor activation
spellingShingle Direct targeting of hippocampal neurons for apoptosis by glucocorticoids is reversible by mineralocorticoid receptor activation
Crochemore, C.
Apoptosis
Dexamethasone
Glucocorticoid receptor
Mineralocorticoid receptor
Spironolactone
RU28318
Hippocampus
Science & Technology
title_short Direct targeting of hippocampal neurons for apoptosis by glucocorticoids is reversible by mineralocorticoid receptor activation
title_full Direct targeting of hippocampal neurons for apoptosis by glucocorticoids is reversible by mineralocorticoid receptor activation
title_fullStr Direct targeting of hippocampal neurons for apoptosis by glucocorticoids is reversible by mineralocorticoid receptor activation
title_full_unstemmed Direct targeting of hippocampal neurons for apoptosis by glucocorticoids is reversible by mineralocorticoid receptor activation
title_sort Direct targeting of hippocampal neurons for apoptosis by glucocorticoids is reversible by mineralocorticoid receptor activation
author Crochemore, C.
author_facet Crochemore, C.
Lu, J.
Wu, Y.
Liposits, Zs.
Sousa, Nuno
Holsboer, F.
Almeida, O. F. X.
author_role author
author2 Lu, J.
Wu, Y.
Liposits, Zs.
Sousa, Nuno
Holsboer, F.
Almeida, O. F. X.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Crochemore, C.
Lu, J.
Wu, Y.
Liposits, Zs.
Sousa, Nuno
Holsboer, F.
Almeida, O. F. X.
dc.subject.por.fl_str_mv Apoptosis
Dexamethasone
Glucocorticoid receptor
Mineralocorticoid receptor
Spironolactone
RU28318
Hippocampus
Science & Technology
topic Apoptosis
Dexamethasone
Glucocorticoid receptor
Mineralocorticoid receptor
Spironolactone
RU28318
Hippocampus
Science & Technology
description Prova tipográfica (In Press)
publishDate 2005
dc.date.none.fl_str_mv 2005-09-16
2005-09-16T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/2944
url http://hdl.handle.net/1822/2944
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1359-4184
10.1038/sj.mp.4001679
15940303
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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