Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates

Detalhes bibliográficos
Autor(a) principal: Cesário, M. Teresa
Data de Publicação: 2014
Outros Autores: Raposo, Rodrigo S., Almeida, M. Catarina M. D. de, Keulen, Frederik van, Ferreira, Bruno S., Fonseca, M. Manuela R. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.26/6276
Resumo: Project: EC/FP7/246449 - New tailor-made PNB-based nanocomposites for high performance applications produced from environmentally friendly production routes
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spelling Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysatesWheat straw hydrolysatesPoly 3-hydroxybutyrateBurkholderia sacchariCarbon catabolite repressionAgricultural lignocellulosic residuesHigh cell density culturesProject: EC/FP7/246449 - New tailor-made PNB-based nanocomposites for high performance applications produced from environmentally friendly production routesNOTICE: this is the author’s version of a work that was accepted for publication in New Biotechnology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in New Biotechnology, [Vol. 31, Issue 1, (January 2014)] DOI 10.1016/j.nbt.2013.10.004"Polyhydroxyalkanoates (PHAs) are bioplastics that can replace conventional petroleum derived products in various applications. One of the major barriers for their widespread introduction in the market is the higher production costs when compared with their petrochemical counterparts. In this work, a process was successfully implemented with high productivity based on wheat straw, a cheap and readily available agricultural residue, as raw material. The strain Burkholderia sacchari DSM 17165 which is able to metabolize glucose, xylose and arabinose, the main sugars present in wheat straw hydrolysates (WSH), was used. Results in shake flask showed that B. sacchari cells accumulated ca 70 % g P(3HB)/g cell-dry-weight with a yield of polymer on sugars (YP/S) of 0.18 g/g when grown on a mixture of commercial C6 and C5 sugars (control), while these values reached ca 60 % g P(3HB)/g cell-dry-weight and 0.19 g/g, respectively, when WSHs were used as carbon source. In fed-batch cultures carried out in 2L stirred tank reactors on WSH, a maximum polymer concentration of 105 g/L was reached after 61 h of cultivation corresponding to an accumulation of 72% of CDW. Polymer yield and productivity were 0.22 g P(3HB)/g total sugar consumed and 1.6 g/L. h, respectively. The selected feeding strategy successfully overcame the carbon catabolite repression phenomenon observed in sugar mixtures containing hexoses and pentoses. This is the first work describing fed-batch cultivations aiming at PHA production using real lignocellulosic hydrolysates. Additionally, the P(3HB) volumetric productivities attained are, by far, the highest achieved ever on agricultural wastes hydrolysates."UEElsevierRepositório ComumCesário, M. TeresaRaposo, Rodrigo S.Almeida, M. Catarina M. D. deKeulen, Frederik vanFerreira, Bruno S.Fonseca, M. Manuela R. da2014-05-05T08:32:04Z2014-01-01T00:00:00Z2014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.26/6276engNew Biotechnology. Volume 31, Issue 1, 25 January 2014, Pages 104–1131871-6784info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-06T14:51:27Zoai:comum.rcaap.pt:10400.26/6276Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:04.049453Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates
title Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates
spellingShingle Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates
Cesário, M. Teresa
Wheat straw hydrolysates
Poly 3-hydroxybutyrate
Burkholderia sacchari
Carbon catabolite repression
Agricultural lignocellulosic residues
High cell density cultures
title_short Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates
title_full Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates
title_fullStr Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates
title_full_unstemmed Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates
title_sort Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates
author Cesário, M. Teresa
author_facet Cesário, M. Teresa
Raposo, Rodrigo S.
Almeida, M. Catarina M. D. de
Keulen, Frederik van
Ferreira, Bruno S.
Fonseca, M. Manuela R. da
author_role author
author2 Raposo, Rodrigo S.
Almeida, M. Catarina M. D. de
Keulen, Frederik van
Ferreira, Bruno S.
Fonseca, M. Manuela R. da
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Comum
dc.contributor.author.fl_str_mv Cesário, M. Teresa
Raposo, Rodrigo S.
Almeida, M. Catarina M. D. de
Keulen, Frederik van
Ferreira, Bruno S.
Fonseca, M. Manuela R. da
dc.subject.por.fl_str_mv Wheat straw hydrolysates
Poly 3-hydroxybutyrate
Burkholderia sacchari
Carbon catabolite repression
Agricultural lignocellulosic residues
High cell density cultures
topic Wheat straw hydrolysates
Poly 3-hydroxybutyrate
Burkholderia sacchari
Carbon catabolite repression
Agricultural lignocellulosic residues
High cell density cultures
description Project: EC/FP7/246449 - New tailor-made PNB-based nanocomposites for high performance applications produced from environmentally friendly production routes
publishDate 2014
dc.date.none.fl_str_mv 2014-05-05T08:32:04Z
2014-01-01T00:00:00Z
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.26/6276
url http://hdl.handle.net/10400.26/6276
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv New Biotechnology. Volume 31, Issue 1, 25 January 2014, Pages 104–113
1871-6784
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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