Trichoderma spp. on treatment of Handroanthus serratifolius seeds: effect on seedling germination and development
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/14099 |
Resumo: | Fungi of the genus Trichoderma are important microorganisms used in biocontrol processes and the promotion of plant development. However, they remain poorly studied in the context of forestry programs, especially those related to native Amazonian species. Thus, it is the aim of this study to evaluate the effects of different Trichoderma isolates on the germination and development of Handroanthus serratifolius seedlings. During in vitro germination tests, seeds were immersed for 24 h in respective fungal suspensions each prepared using one of five Trichoderma isolates. The suspensions were held in plastic trays and kept at a temperature of 24 ± 2 °C. Metrics related to germination and development assessed under laboratory conditions include: germination speed index (GSI), germination percentage, length of the roots, and hypocotyls, as well as fungal perseverance. In the nursery, Trichoderma were used in two different applications: pre-planting treatment and as a monthly, post-planting treatment. Pre-planting treatments consisted of 10 g of colonized rice grains bearing each isolate being placed into experimental bags five days before seeding. The post-planting treatment involved the application of 10 mL of fungal suspension per experimental bag. Each month, the height, stem diameter, and leaf number were measured for each seedling. At the end of the experiment, the length and mass of roots as well as the total dry mass were recorded. In laboratory conditions, seeds treated with Trichoderma asperellum -TAM03 obtained the greatest fractional germination (76.5%) and GSI. In the nursery experiments, isolates TAM01 and TAM03, when applied as a post-planting treatment, increased the height, stem diameter, and number of leaves of treated plants with respect to the control group by 180 days post-treatment. After 365 days, plants which received TAM01 pre-planting treatments were observed to have increased root and aerial part length, as well as root mass and overall dry mass. These results suggest that T. asperellum -TAM01 positively affects H. serratifolius development. |
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spelling |
Trichoderma spp. on treatment of Handroanthus serratifolius seeds: effect on seedling germination and developmentBiotechnologyGrowth promotionBiological treatmentBiological scienceMicrobiologyPhysiologyPlant biologyEnvironmental scienceSustainable forestrySeedling productionYellow ipeFungi of the genus Trichoderma are important microorganisms used in biocontrol processes and the promotion of plant development. However, they remain poorly studied in the context of forestry programs, especially those related to native Amazonian species. Thus, it is the aim of this study to evaluate the effects of different Trichoderma isolates on the germination and development of Handroanthus serratifolius seedlings. During in vitro germination tests, seeds were immersed for 24 h in respective fungal suspensions each prepared using one of five Trichoderma isolates. The suspensions were held in plastic trays and kept at a temperature of 24 ± 2 °C. Metrics related to germination and development assessed under laboratory conditions include: germination speed index (GSI), germination percentage, length of the roots, and hypocotyls, as well as fungal perseverance. In the nursery, Trichoderma were used in two different applications: pre-planting treatment and as a monthly, post-planting treatment. Pre-planting treatments consisted of 10 g of colonized rice grains bearing each isolate being placed into experimental bags five days before seeding. The post-planting treatment involved the application of 10 mL of fungal suspension per experimental bag. Each month, the height, stem diameter, and leaf number were measured for each seedling. At the end of the experiment, the length and mass of roots as well as the total dry mass were recorded. In laboratory conditions, seeds treated with Trichoderma asperellum -TAM03 obtained the greatest fractional germination (76.5%) and GSI. In the nursery experiments, isolates TAM01 and TAM03, when applied as a post-planting treatment, increased the height, stem diameter, and number of leaves of treated plants with respect to the control group by 180 days post-treatment. After 365 days, plants which received TAM01 pre-planting treatments were observed to have increased root and aerial part length, as well as root mass and overall dry mass. These results suggest that T. asperellum -TAM01 positively affects H. serratifolius development.Institutional Scientific Initiation Scholarship Program (PIBIC/UFOPA)ElsevierSapientiaSantos, Misael Freitas dosSantos, Lizandra Elizeário dosCosta, Daniele Lima daVieira, T.A.Lustosa, Denise Castro2020-07-17T13:03:35Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/14099eng2405-844010.1016/j.heliyon.2020.e04044info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:26:20Zoai:sapientia.ualg.pt:10400.1/14099Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:05:09.291960Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Trichoderma spp. on treatment of Handroanthus serratifolius seeds: effect on seedling germination and development |
title |
Trichoderma spp. on treatment of Handroanthus serratifolius seeds: effect on seedling germination and development |
spellingShingle |
Trichoderma spp. on treatment of Handroanthus serratifolius seeds: effect on seedling germination and development Santos, Misael Freitas dos Biotechnology Growth promotion Biological treatment Biological science Microbiology Physiology Plant biology Environmental science Sustainable forestry Seedling production Yellow ipe |
title_short |
Trichoderma spp. on treatment of Handroanthus serratifolius seeds: effect on seedling germination and development |
title_full |
Trichoderma spp. on treatment of Handroanthus serratifolius seeds: effect on seedling germination and development |
title_fullStr |
Trichoderma spp. on treatment of Handroanthus serratifolius seeds: effect on seedling germination and development |
title_full_unstemmed |
Trichoderma spp. on treatment of Handroanthus serratifolius seeds: effect on seedling germination and development |
title_sort |
Trichoderma spp. on treatment of Handroanthus serratifolius seeds: effect on seedling germination and development |
author |
Santos, Misael Freitas dos |
author_facet |
Santos, Misael Freitas dos Santos, Lizandra Elizeário dos Costa, Daniele Lima da Vieira, T.A. Lustosa, Denise Castro |
author_role |
author |
author2 |
Santos, Lizandra Elizeário dos Costa, Daniele Lima da Vieira, T.A. Lustosa, Denise Castro |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Santos, Misael Freitas dos Santos, Lizandra Elizeário dos Costa, Daniele Lima da Vieira, T.A. Lustosa, Denise Castro |
dc.subject.por.fl_str_mv |
Biotechnology Growth promotion Biological treatment Biological science Microbiology Physiology Plant biology Environmental science Sustainable forestry Seedling production Yellow ipe |
topic |
Biotechnology Growth promotion Biological treatment Biological science Microbiology Physiology Plant biology Environmental science Sustainable forestry Seedling production Yellow ipe |
description |
Fungi of the genus Trichoderma are important microorganisms used in biocontrol processes and the promotion of plant development. However, they remain poorly studied in the context of forestry programs, especially those related to native Amazonian species. Thus, it is the aim of this study to evaluate the effects of different Trichoderma isolates on the germination and development of Handroanthus serratifolius seedlings. During in vitro germination tests, seeds were immersed for 24 h in respective fungal suspensions each prepared using one of five Trichoderma isolates. The suspensions were held in plastic trays and kept at a temperature of 24 ± 2 °C. Metrics related to germination and development assessed under laboratory conditions include: germination speed index (GSI), germination percentage, length of the roots, and hypocotyls, as well as fungal perseverance. In the nursery, Trichoderma were used in two different applications: pre-planting treatment and as a monthly, post-planting treatment. Pre-planting treatments consisted of 10 g of colonized rice grains bearing each isolate being placed into experimental bags five days before seeding. The post-planting treatment involved the application of 10 mL of fungal suspension per experimental bag. Each month, the height, stem diameter, and leaf number were measured for each seedling. At the end of the experiment, the length and mass of roots as well as the total dry mass were recorded. In laboratory conditions, seeds treated with Trichoderma asperellum -TAM03 obtained the greatest fractional germination (76.5%) and GSI. In the nursery experiments, isolates TAM01 and TAM03, when applied as a post-planting treatment, increased the height, stem diameter, and number of leaves of treated plants with respect to the control group by 180 days post-treatment. After 365 days, plants which received TAM01 pre-planting treatments were observed to have increased root and aerial part length, as well as root mass and overall dry mass. These results suggest that T. asperellum -TAM01 positively affects H. serratifolius development. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07-17T13:03:35Z 2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/14099 |
url |
http://hdl.handle.net/10400.1/14099 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2405-8440 10.1016/j.heliyon.2020.e04044 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133290003693568 |