Enhancement of fermentative hydrogen production from Spirogyra sp by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model

Detalhes bibliográficos
Autor(a) principal: Pinto, T.
Data de Publicação: 2018
Outros Autores: Gouveia, Luisa, Ortigueira, Joana, Saratale, Ganesh D., Moura, Patrícia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.9/3060
Resumo: ABSTRACT: In this work, hydrogen (H-2) was produced through the fermentation of Spirogyra sp. biomass by Clostridium butyricum DSM 10702. Macronutrient stress was applied to increase the carbohydrate content in Spirogyra, and a 36% (w/w) accumulation of carbohydrates was reached by nitrogen depletion. The use of wet microalga as fermentable substrate was compared with physically and chemically treated biomass for increased carbohydrate solubilisation. The combination of drying, bead beating and mild acid hydrolysis produced a saccharification yield of 90.3% (w/w). The H-2 production from Spirogyra hydrolysate was 3.9 L H-2 L-1 , equivalent to 1463 mL H-2 g(-1) microalga dry weight. The presence of protein (23.2 +/- 0.3% w/w) and valuable pigments, such as astaxanthin (38.8% of the total pigment content), makes this microalga suitable to be used simultaneously in both food and feed applications. In a Spirogyra based biorefinery, the potential energy production and food-grade protein and pigments revenue per cubic meter of microalga culture per year was estimated on 7.4 MJ, US $412 and US S15, respectively, thereby contributing to the cost efficiency and sustainability of the whole bioconversion process.
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spelling Enhancement of fermentative hydrogen production from Spirogyra sp by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery modelMicroalgaeSpirogyra sp.Clostridium butyricumDark fermentationPigmentsBioethanolBiofuelsABSTRACT: In this work, hydrogen (H-2) was produced through the fermentation of Spirogyra sp. biomass by Clostridium butyricum DSM 10702. Macronutrient stress was applied to increase the carbohydrate content in Spirogyra, and a 36% (w/w) accumulation of carbohydrates was reached by nitrogen depletion. The use of wet microalga as fermentable substrate was compared with physically and chemically treated biomass for increased carbohydrate solubilisation. The combination of drying, bead beating and mild acid hydrolysis produced a saccharification yield of 90.3% (w/w). The H-2 production from Spirogyra hydrolysate was 3.9 L H-2 L-1 , equivalent to 1463 mL H-2 g(-1) microalga dry weight. The presence of protein (23.2 +/- 0.3% w/w) and valuable pigments, such as astaxanthin (38.8% of the total pigment content), makes this microalga suitable to be used simultaneously in both food and feed applications. In a Spirogyra based biorefinery, the potential energy production and food-grade protein and pigments revenue per cubic meter of microalga culture per year was estimated on 7.4 MJ, US $412 and US S15, respectively, thereby contributing to the cost efficiency and sustainability of the whole bioconversion process.ElsevierRepositório do LNEGPinto, T.Gouveia, LuisaOrtigueira, JoanaSaratale, Ganesh D.Moura, Patrícia2018-09-19T14:33:08Z2018-01-01T00:00:00Z2018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/3060engPinto, Tiago; Gouveia, Luisa; Saratale, Ganesh D.; [el.al.]. - Enhancement of fermentative hydrogen production from Spirogyra sp by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model. In: Journal of Bioscience and Bioengineering, 2018, Vol. 126 (2), p. 226-2341389-1723info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T12:28:25Zoai:repositorio.lneg.pt:10400.9/3060Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:36:13.173788Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Enhancement of fermentative hydrogen production from Spirogyra sp by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model
title Enhancement of fermentative hydrogen production from Spirogyra sp by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model
spellingShingle Enhancement of fermentative hydrogen production from Spirogyra sp by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model
Pinto, T.
Microalgae
Spirogyra sp.
Clostridium butyricum
Dark fermentation
Pigments
Bioethanol
Biofuels
title_short Enhancement of fermentative hydrogen production from Spirogyra sp by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model
title_full Enhancement of fermentative hydrogen production from Spirogyra sp by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model
title_fullStr Enhancement of fermentative hydrogen production from Spirogyra sp by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model
title_full_unstemmed Enhancement of fermentative hydrogen production from Spirogyra sp by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model
title_sort Enhancement of fermentative hydrogen production from Spirogyra sp by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model
author Pinto, T.
author_facet Pinto, T.
Gouveia, Luisa
Ortigueira, Joana
Saratale, Ganesh D.
Moura, Patrícia
author_role author
author2 Gouveia, Luisa
Ortigueira, Joana
Saratale, Ganesh D.
Moura, Patrícia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório do LNEG
dc.contributor.author.fl_str_mv Pinto, T.
Gouveia, Luisa
Ortigueira, Joana
Saratale, Ganesh D.
Moura, Patrícia
dc.subject.por.fl_str_mv Microalgae
Spirogyra sp.
Clostridium butyricum
Dark fermentation
Pigments
Bioethanol
Biofuels
topic Microalgae
Spirogyra sp.
Clostridium butyricum
Dark fermentation
Pigments
Bioethanol
Biofuels
description ABSTRACT: In this work, hydrogen (H-2) was produced through the fermentation of Spirogyra sp. biomass by Clostridium butyricum DSM 10702. Macronutrient stress was applied to increase the carbohydrate content in Spirogyra, and a 36% (w/w) accumulation of carbohydrates was reached by nitrogen depletion. The use of wet microalga as fermentable substrate was compared with physically and chemically treated biomass for increased carbohydrate solubilisation. The combination of drying, bead beating and mild acid hydrolysis produced a saccharification yield of 90.3% (w/w). The H-2 production from Spirogyra hydrolysate was 3.9 L H-2 L-1 , equivalent to 1463 mL H-2 g(-1) microalga dry weight. The presence of protein (23.2 +/- 0.3% w/w) and valuable pigments, such as astaxanthin (38.8% of the total pigment content), makes this microalga suitable to be used simultaneously in both food and feed applications. In a Spirogyra based biorefinery, the potential energy production and food-grade protein and pigments revenue per cubic meter of microalga culture per year was estimated on 7.4 MJ, US $412 and US S15, respectively, thereby contributing to the cost efficiency and sustainability of the whole bioconversion process.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-19T14:33:08Z
2018-01-01T00:00:00Z
2018-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.9/3060
url http://hdl.handle.net/10400.9/3060
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pinto, Tiago; Gouveia, Luisa; Saratale, Ganesh D.; [el.al.]. - Enhancement of fermentative hydrogen production from Spirogyra sp by increased carbohydrate accumulation and selection of the biomass pretreatment under a biorefinery model. In: Journal of Bioscience and Bioengineering, 2018, Vol. 126 (2), p. 226-234
1389-1723
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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