Cellular antioxidant, anti-inflammatory, and antiproliferative activities from the flowers, leaves and fruits of Gallesia integrifolia spreng harms

• Autor(a) principal: Silva, Gabriela Catuzo Canonico
• Data de Publicação: 2012
• Outros Autores: Machado, Mariane de Almeida, Sakumoto, Karina, Inumaro, Rodrigo Sadao, Gonçalves, José Eduardo, Mandim, Filipa, Vaz, Josiana A., Valle, Juliana Silveira do, Faria, Maria Graciela Iecher, Ruiz, Suelen Pereira, Piau Junior, Ranulfo, Gonçalves, Daniela Dib, Gazim, Zilda Cristiani
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10198/6684

Gallesia integrifolia, a notable species in the Atlantic Forest, has been traditionally employed in folk medicine for treating rheumatism, asthma, and worms. This study investigated the cellular antioxidant, antiproliferative, and anti-inflammatory activities of the essential oils (EOs) and crude extracts (CEs) from G. integrifolia flowers, fruits, and leaves. The chemical identification of EOs was performed by GC-MS and CEs by UHPLC-MS. Cellular antioxidant and anti-inflammatory activities were assessed through mouse macrophage cell culture. In addition, the antiproliferative potential was evaluated in gastric, colorectal, breast, and lung tumor cell lines and non-tumor VERO cells. EOs predominantly contained organosulfur compounds in flowers (96.29%), fruits (94.94%), and leaves (90.72%). We found the main compound is 2,2 & PRIME;-Disulfanediyldiethanethiol in the EOs of flowers (47.00%), leaves (41.82%), and fruits (44.39%). Phenolic compounds were identified in CEs. The EOs and CEs demonstrated potential against the tumor cell lines tested (GI(50) between 51 and 230 & mu;g/mL). The selectivity index values were greater than 1.0 (1.01 to 3.37), suggesting a relative safety profile. Moreover, the anti-inflammatory activity IC50 ranged from 36.00 to 268 & mu;g/mL, and the cellular oxidation inhibition ranged from 69% to 82%. The results suggest that oils and extracts derived from G. integrifolia have potential for use in various industrial sectors.