Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfaces
Autor(a) principal: | |
---|---|
Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5119 https://doi.org/10.1016/j.bios.2004.06.014 |
Resumo: | Complexes between short oligodeoxynucleotides (ODN) with a variable dGxdCy base composition and liposomes composed of the cationic lipid DOTAP (ODN lipoplexes) were studied by differential pulse voltammetry at a glassy carbon electrode. Since lipoplexes are spontaneously formed by electrostatic interactions, the objective of the voltammetric study was to investigate their behaviour at the electrode surface/solution interface. It was verified that the peak current in the voltammograms for ODN lipoplexes was due to guanosine oxidation and that it was influenced both by the applied adsorption potential and the lipoplex (±) charge ratio used. It was found that for low ODN lipoplexes (±) charge ratios the peak current obtained was enhanced when compared to that registered with free ODN for the same concentration. This allowed a higher sensitivity in the determination of ODN by differential pulse voltammetry and a limit of detection of 5.5 ng/mL was achieved. A model that explains the organisation of ODN lipoplexes at the electrode surface/solution interface is proposed. The electrochemical results presented account for a better physicochemical characterisation of lipoplexes at charged interfaces, which can be important for the understanding and development of gene therapy vectors based on ODN lipoplexes. |
id |
RCAP_983f2ac6522d355a6e766ca7a5fdc6f3 |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/5119 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfacesVoltammetryCharged interfaceOligonucleotide lipoplexCationic liposomeComplexes between short oligodeoxynucleotides (ODN) with a variable dGxdCy base composition and liposomes composed of the cationic lipid DOTAP (ODN lipoplexes) were studied by differential pulse voltammetry at a glassy carbon electrode. Since lipoplexes are spontaneously formed by electrostatic interactions, the objective of the voltammetric study was to investigate their behaviour at the electrode surface/solution interface. It was verified that the peak current in the voltammograms for ODN lipoplexes was due to guanosine oxidation and that it was influenced both by the applied adsorption potential and the lipoplex (±) charge ratio used. It was found that for low ODN lipoplexes (±) charge ratios the peak current obtained was enhanced when compared to that registered with free ODN for the same concentration. This allowed a higher sensitivity in the determination of ODN by differential pulse voltammetry and a limit of detection of 5.5 ng/mL was achieved. A model that explains the organisation of ODN lipoplexes at the electrode surface/solution interface is proposed. The electrochemical results presented account for a better physicochemical characterisation of lipoplexes at charged interfaces, which can be important for the understanding and development of gene therapy vectors based on ODN lipoplexes.http://www.sciencedirect.com/science/article/B6TFC-4CYNVTG-4/1/431916a28ef62a0aab03cde700cdf1142004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5119http://hdl.handle.net/10316/5119https://doi.org/10.1016/j.bios.2004.06.014engBiosensors and Bioelectronics. 20:5 (2004) 975-984Piedade, J. A. P.Mano, M.Lima, M. C. Pedroso deOretskaya, T. S.Oliveira-Brett, A. M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-09-17T11:25:14Zoai:estudogeral.uc.pt:10316/5119Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:40.076623Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfaces |
title |
Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfaces |
spellingShingle |
Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfaces Piedade, J. A. P. Voltammetry Charged interface Oligonucleotide lipoplex Cationic liposome |
title_short |
Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfaces |
title_full |
Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfaces |
title_fullStr |
Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfaces |
title_full_unstemmed |
Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfaces |
title_sort |
Electrochemical sensing of the behaviour of oligonucleotide lipoplexes at charged interfaces |
author |
Piedade, J. A. P. |
author_facet |
Piedade, J. A. P. Mano, M. Lima, M. C. Pedroso de Oretskaya, T. S. Oliveira-Brett, A. M. |
author_role |
author |
author2 |
Mano, M. Lima, M. C. Pedroso de Oretskaya, T. S. Oliveira-Brett, A. M. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Piedade, J. A. P. Mano, M. Lima, M. C. Pedroso de Oretskaya, T. S. Oliveira-Brett, A. M. |
dc.subject.por.fl_str_mv |
Voltammetry Charged interface Oligonucleotide lipoplex Cationic liposome |
topic |
Voltammetry Charged interface Oligonucleotide lipoplex Cationic liposome |
description |
Complexes between short oligodeoxynucleotides (ODN) with a variable dGxdCy base composition and liposomes composed of the cationic lipid DOTAP (ODN lipoplexes) were studied by differential pulse voltammetry at a glassy carbon electrode. Since lipoplexes are spontaneously formed by electrostatic interactions, the objective of the voltammetric study was to investigate their behaviour at the electrode surface/solution interface. It was verified that the peak current in the voltammograms for ODN lipoplexes was due to guanosine oxidation and that it was influenced both by the applied adsorption potential and the lipoplex (±) charge ratio used. It was found that for low ODN lipoplexes (±) charge ratios the peak current obtained was enhanced when compared to that registered with free ODN for the same concentration. This allowed a higher sensitivity in the determination of ODN by differential pulse voltammetry and a limit of detection of 5.5 ng/mL was achieved. A model that explains the organisation of ODN lipoplexes at the electrode surface/solution interface is proposed. The electrochemical results presented account for a better physicochemical characterisation of lipoplexes at charged interfaces, which can be important for the understanding and development of gene therapy vectors based on ODN lipoplexes. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5119 http://hdl.handle.net/10316/5119 https://doi.org/10.1016/j.bios.2004.06.014 |
url |
http://hdl.handle.net/10316/5119 https://doi.org/10.1016/j.bios.2004.06.014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biosensors and Bioelectronics. 20:5 (2004) 975-984 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133907433553920 |