Development and application of a novel peptide nucleic acid probe for the specific detection of helicobacter pylori in gastric biopsy specimens

Detalhes bibliográficos
Autor(a) principal: Guimarães, N.
Data de Publicação: 2007
Outros Autores: Azevedo, N. F., Figueiredo, C., Keevil, C. W., Vieira, M. J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/6999
Resumo: In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffinembedded gastric biopsy specimens.
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spelling Development and application of a novel peptide nucleic acid probe for the specific detection of helicobacter pylori in gastric biopsy specimensScience & TechnologyIn this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffinembedded gastric biopsy specimens.Fundação para a Ciência e a Tecnologia (FCT)European Commission Research Project SAFERAmerican Society for Microbiology (ASM)Universidade do MinhoGuimarães, N.Azevedo, N. F.Figueiredo, C.Keevil, C. W.Vieira, M. J.2007-092007-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/6999eng"Journal of Clinical Microbiology". ISSN 0095-1137. 45:9 (Sept. 2007) 3089-3094.0095-113710.1128/JCM.00858-0717609326info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:01Zoai:repositorium.sdum.uminho.pt:1822/6999Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:41:37.424420Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development and application of a novel peptide nucleic acid probe for the specific detection of helicobacter pylori in gastric biopsy specimens
title Development and application of a novel peptide nucleic acid probe for the specific detection of helicobacter pylori in gastric biopsy specimens
spellingShingle Development and application of a novel peptide nucleic acid probe for the specific detection of helicobacter pylori in gastric biopsy specimens
Guimarães, N.
Science & Technology
title_short Development and application of a novel peptide nucleic acid probe for the specific detection of helicobacter pylori in gastric biopsy specimens
title_full Development and application of a novel peptide nucleic acid probe for the specific detection of helicobacter pylori in gastric biopsy specimens
title_fullStr Development and application of a novel peptide nucleic acid probe for the specific detection of helicobacter pylori in gastric biopsy specimens
title_full_unstemmed Development and application of a novel peptide nucleic acid probe for the specific detection of helicobacter pylori in gastric biopsy specimens
title_sort Development and application of a novel peptide nucleic acid probe for the specific detection of helicobacter pylori in gastric biopsy specimens
author Guimarães, N.
author_facet Guimarães, N.
Azevedo, N. F.
Figueiredo, C.
Keevil, C. W.
Vieira, M. J.
author_role author
author2 Azevedo, N. F.
Figueiredo, C.
Keevil, C. W.
Vieira, M. J.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Guimarães, N.
Azevedo, N. F.
Figueiredo, C.
Keevil, C. W.
Vieira, M. J.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffinembedded gastric biopsy specimens.
publishDate 2007
dc.date.none.fl_str_mv 2007-09
2007-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/6999
url http://hdl.handle.net/1822/6999
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of Clinical Microbiology". ISSN 0095-1137. 45:9 (Sept. 2007) 3089-3094.
0095-1137
10.1128/JCM.00858-07
17609326
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology (ASM)
publisher.none.fl_str_mv American Society for Microbiology (ASM)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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