Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, Portugal

Detalhes bibliográficos
Autor(a) principal: Peixoto, Francisco P.
Data de Publicação: 2013
Outros Autores: Carrola, João, Coimbra, Ana Maria, Fernandes, Conceição, Teixeira, Paulo, Coelho, Luís, Conceição, Ivo, Oliveira, Maria Manuel, Fontaínhas-Fernandes, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/10242
Resumo: Barbel (Barbus bocagei) a common species in Portuguese rivers was studied to assess the impact of water contamination on hepatic oxidative stress response, lipid peroxidation and histology. The Vizela River is a tributary of the Ave River, located in one of the most industrialized areas of Portugal. The oxidative stress biomarkers analyzed included superoxide dismutase, catalase, glutathione S-transferase, glutathione reductase, glucose 6‑phosphate dehydrogenase and xanthine oxidase activities. Levels of reduced glutathione and lipid peroxidation were also evaluated. Except xanthine oxidase activity, that did not show any alteration, all the other enzymatic activities were increased in the liver of barbel captured in the Vizela River when compared with reference barbel. While, no differences were observed for glutathione reductase content, lipid peroxidation was higher in barbel from the Vizela River. Liver histological alterations were determined and their severity scored. Though lymphocyte foci were only observed in Vizela River barbel, macrophage aggregates were also present in reference barbel, although the severity score was higher in Vizela fish. The results of this study show that barbel liver oxidative stress responses, lipid peroxidation and histology are sensitive to the contaminants present in Vizela River water and are valuable biomarkers for monitoring purposes.
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spelling Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, PortugalOxidative stressBarbus bocageiVizela riverLiver histopathologyLipid peroxidationPortugalBarbel (Barbus bocagei) a common species in Portuguese rivers was studied to assess the impact of water contamination on hepatic oxidative stress response, lipid peroxidation and histology. The Vizela River is a tributary of the Ave River, located in one of the most industrialized areas of Portugal. The oxidative stress biomarkers analyzed included superoxide dismutase, catalase, glutathione S-transferase, glutathione reductase, glucose 6‑phosphate dehydrogenase and xanthine oxidase activities. Levels of reduced glutathione and lipid peroxidation were also evaluated. Except xanthine oxidase activity, that did not show any alteration, all the other enzymatic activities were increased in the liver of barbel captured in the Vizela River when compared with reference barbel. While, no differences were observed for glutathione reductase content, lipid peroxidation was higher in barbel from the Vizela River. Liver histological alterations were determined and their severity scored. Though lymphocyte foci were only observed in Vizela River barbel, macrophage aggregates were also present in reference barbel, although the severity score was higher in Vizela fish. The results of this study show that barbel liver oxidative stress responses, lipid peroxidation and histology are sensitive to the contaminants present in Vizela River water and are valuable biomarkers for monitoring purposes.Barbos (Barbus bocagei), una especie común en los ríos portugueses, se utilizó para evaluar el impacto de la contaminación del agua en la respuesta hepática al estrés oxidativo, en la peroxidación lipídica y en la histología del órgano. El río Vizela es un afluente del río Ave, situado en una de las regiones más industrializadas de Portugal. Los biomarcadores de estrés oxidativo analizados fueron la actividad de las enzimas superóxido dismutasa, catalasa, glutatión S-transferasa, glutation reductasa, glucosa 6 fosfato deshidrogenasa y de la xantina oxidasa. Los niveles de glutatión reducido y de la peroxidación lipídica también fueron evaluados. Excepto la xantina oxidasa, que no mostró ninguna alteración, todas las otras actividades enzimáticas han sufrido incrementos en el hígado de los barbos capturados en el río Vizela, cuando se comparan con los barbos de referencia. No se observaron diferencias para el contenido de glutatión reductasa, pero la peroxidación lipídica fue mayor en los barbos del río Vizela. Las alteraciones en la histología hepática fueron identificadas y clasificadas de acuerdo con su gravedad. Mientras que los linfocitos de focos se observaron sólo en barbos del Río Vizela, los agregados de macrófagos también estuvieron presentes en barbos locales de referencia, aunque la gravedad de las alteraciones fue mayor en los peces del río Vizela. Los resultados de este estudio muestran que las respuestas de estrés oxidativo, la peroxidación lipídica y la histología hepática son sensibles a los contaminantes presentes en el agua del Río Vizela, demonstrando ser biomarcadores valiosos para propósitos de monitoreo.Biblioteca Digital do IPBPeixoto, Francisco P.Carrola, JoãoCoimbra, Ana MariaFernandes, ConceiçãoTeixeira, PauloCoelho, LuísConceição, IvoOliveira, Maria ManuelFontaínhas-Fernandes, A.2014-08-28T11:13:43Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/10242engPeixoto, Francisco P.; Carrola, J.S.; Coimbra, A.; Fernandes, Conceição; Teixeira, P.; Coelho, L.; Conceição, Ivo; Oliveira, Maria Manuel; Fontainhas-Fernandes, A. (2013). Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, Portugal. Revista Internacional de Contaminación Ambiental. ISSN 0188-4999. 29:1, p. 29-38.0188-4999info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:24:38Zoai:bibliotecadigital.ipb.pt:10198/10242Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:01:14.070266Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, Portugal
title Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, Portugal
spellingShingle Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, Portugal
Peixoto, Francisco P.
Oxidative stress
Barbus bocagei
Vizela river
Liver histopathology
Lipid peroxidation
Portugal
title_short Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, Portugal
title_full Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, Portugal
title_fullStr Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, Portugal
title_full_unstemmed Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, Portugal
title_sort Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, Portugal
author Peixoto, Francisco P.
author_facet Peixoto, Francisco P.
Carrola, João
Coimbra, Ana Maria
Fernandes, Conceição
Teixeira, Paulo
Coelho, Luís
Conceição, Ivo
Oliveira, Maria Manuel
Fontaínhas-Fernandes, A.
author_role author
author2 Carrola, João
Coimbra, Ana Maria
Fernandes, Conceição
Teixeira, Paulo
Coelho, Luís
Conceição, Ivo
Oliveira, Maria Manuel
Fontaínhas-Fernandes, A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Peixoto, Francisco P.
Carrola, João
Coimbra, Ana Maria
Fernandes, Conceição
Teixeira, Paulo
Coelho, Luís
Conceição, Ivo
Oliveira, Maria Manuel
Fontaínhas-Fernandes, A.
dc.subject.por.fl_str_mv Oxidative stress
Barbus bocagei
Vizela river
Liver histopathology
Lipid peroxidation
Portugal
topic Oxidative stress
Barbus bocagei
Vizela river
Liver histopathology
Lipid peroxidation
Portugal
description Barbel (Barbus bocagei) a common species in Portuguese rivers was studied to assess the impact of water contamination on hepatic oxidative stress response, lipid peroxidation and histology. The Vizela River is a tributary of the Ave River, located in one of the most industrialized areas of Portugal. The oxidative stress biomarkers analyzed included superoxide dismutase, catalase, glutathione S-transferase, glutathione reductase, glucose 6‑phosphate dehydrogenase and xanthine oxidase activities. Levels of reduced glutathione and lipid peroxidation were also evaluated. Except xanthine oxidase activity, that did not show any alteration, all the other enzymatic activities were increased in the liver of barbel captured in the Vizela River when compared with reference barbel. While, no differences were observed for glutathione reductase content, lipid peroxidation was higher in barbel from the Vizela River. Liver histological alterations were determined and their severity scored. Though lymphocyte foci were only observed in Vizela River barbel, macrophage aggregates were also present in reference barbel, although the severity score was higher in Vizela fish. The results of this study show that barbel liver oxidative stress responses, lipid peroxidation and histology are sensitive to the contaminants present in Vizela River water and are valuable biomarkers for monitoring purposes.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2014-08-28T11:13:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/10242
url http://hdl.handle.net/10198/10242
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Peixoto, Francisco P.; Carrola, J.S.; Coimbra, A.; Fernandes, Conceição; Teixeira, P.; Coelho, L.; Conceição, Ivo; Oliveira, Maria Manuel; Fontainhas-Fernandes, A. (2013). Oxidative stress responses and histological hepatic alterations in barbel, Barbus bocagei, from Vizela river, Portugal. Revista Internacional de Contaminación Ambiental. ISSN 0188-4999. 29:1, p. 29-38.
0188-4999
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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