Unveiling co-infection in cystic fibrosis airways: transcriptomic analysis of Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilms

Detalhes bibliográficos
Autor(a) principal: Magalhães, Andreia Patrícia Alves
Data de Publicação: 2022
Outros Autores: França, Ângela Maria Oliveira Sousa, Pereira, Maria Olívia, Cerca, Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/79271
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fgene.2022.883199/ full#supplementary-material
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spelling Unveiling co-infection in cystic fibrosis airways: transcriptomic analysis of Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilmsPseudomonas aeruginosaStaphylococcus aureusRNA sequencingdual-species biofilmscystic fibrosisScience & TechnologyThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fgene.2022.883199/ full#supplementary-materialCystic fibrosis (CF) is a common heritable genetic disorder caused by a defect in the cystic fibrosis conductance regulator gene, resulting in several complications in the human body (Kreda et al., 2012). So far, the pathological changes in the lungs are best studied due to the high mortality rates linked to poor lung function and the recurrent development of severe biofilm-related infections (Flume et al., 2009; Ciofu et al., 2015). Staphylococcus aureus and Pseudomonas aeruginosa are the most prevalent pathogens that colonize structurally abnormal airways such as those diagnosed with CF and other chronic obstructive lung diseases (Lyczak et al., 2002; Hubert et al., 2013). Although these bacteria seem to succeed with one another, CF patients acquire coinciding P. aeruginosa and S. aureus pulmonary infections, being co-infection usually associated with decreased lung function and increased frequency of pulmonary exacerbations (Limoli et al., 2016). Furthermore, P. aeruginosa and S. aureus pathogens adopt a biofilm mode of growth, which contributes to high tolerance to antibiotic treatment (Schobert and Jahn, 2010) and the recalcitrant nature of these chronic co-infections (Burmølle et al., 2006; Lopes et al., 2012), leading to significant patient morbidity and mortality (Cox et al., 2010). Interactions between P. aeruginosa and S. aureus have been widely studied, and it is commonly admitted that P. aeruginosa outcompetes S. aureus, perhaps outcompeting S. aureus for limited nutrients (Mashburn et al., 2005) or producing antistaphylococcal compounds (DeLeon et al., 2014; Fugère et al., 2014), having S. aureus a minimal contribution to the overall course of the CF-associated biofilm infections (Bragonzi et al., 2012; Filkins et al., 2015). However, P. aeruginosa and S. aureus have been identified in the same lobe of CF lungs (Hogan et al., 2016; Wakeman et al., 2016) and are frequently diagnosed (Limoli et al., 2016; Zolin et al., 2019) as co-infecting species in CF patients. Moreover, P. aeruginosa strains isolated from early infection outcompete S. aureus, while strains isolated from chronic infection are less aggressive and can be co-cultivated with S. aureus (Frydenlund Michelsen et al., 2016; Limoli et al., 2017), suggesting that these pathogens can interact in vivo. In a previous study, we showed that S. aureus can grow and coexist with P. aeruginosa under dualspecies biofilm conditions (Magalhães et al., 2021). Following up on these findings, and acknowledging that the molecular mechanisms behind these interactions are largely unknown, the purpose of the present study was, therefore, to identify the major transcriptomic features of P. aeruginosaS. aureus dual-species biofilms, using high-throughput RNA-sequencing (RNA-seq). Herein, we described the full transcriptome of P. aeruginosa and S. aureus single- and dual-species biofilms and used a data analysis approach based on direct and functional gene interactions, namely gene set enrichment. These results will be invaluable for future functional studies involving P. aeruginosaS. aureus interactions.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2020 unit and the BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020–Programa Operacional Regional do Norte. The authors also acknowledge the support, through the Programa Operacional Competitividade e Internacionalização (COMPETE 2020) and by national funds, through FCT, of the PhD Grant of APM (SFRH/BD/132165/2017).info:eu-repo/semantics/publishedVersionFrontiers MediaUniversidade do MinhoMagalhães, Andreia Patrícia AlvesFrança, Ângela Maria Oliveira SousaPereira, Maria OlíviaCerca, Nuno2022-07-062022-07-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/79271engMagalhães, Andreia P.; França, Angela; Pereira, Maria Olívia; Cerca, Nuno, Unveiling co-infection in cystic fibrosis airways: transcriptomic analysis of Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilms. Frontiers in Genetics, 13(883199), 20221664-802110.3389/fgene.2022.883199883199https://www.frontiersin.org/articles/10.3389/fgene.2022.883199info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:45:15Zoai:repositorium.sdum.uminho.pt:1822/79271Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:43:05.310032Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Unveiling co-infection in cystic fibrosis airways: transcriptomic analysis of Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilms
title Unveiling co-infection in cystic fibrosis airways: transcriptomic analysis of Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilms
spellingShingle Unveiling co-infection in cystic fibrosis airways: transcriptomic analysis of Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilms
Magalhães, Andreia Patrícia Alves
Pseudomonas aeruginosa
Staphylococcus aureus
RNA sequencing
dual-species biofilms
cystic fibrosis
Science & Technology
title_short Unveiling co-infection in cystic fibrosis airways: transcriptomic analysis of Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilms
title_full Unveiling co-infection in cystic fibrosis airways: transcriptomic analysis of Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilms
title_fullStr Unveiling co-infection in cystic fibrosis airways: transcriptomic analysis of Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilms
title_full_unstemmed Unveiling co-infection in cystic fibrosis airways: transcriptomic analysis of Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilms
title_sort Unveiling co-infection in cystic fibrosis airways: transcriptomic analysis of Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilms
author Magalhães, Andreia Patrícia Alves
author_facet Magalhães, Andreia Patrícia Alves
França, Ângela Maria Oliveira Sousa
Pereira, Maria Olívia
Cerca, Nuno
author_role author
author2 França, Ângela Maria Oliveira Sousa
Pereira, Maria Olívia
Cerca, Nuno
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Magalhães, Andreia Patrícia Alves
França, Ângela Maria Oliveira Sousa
Pereira, Maria Olívia
Cerca, Nuno
dc.subject.por.fl_str_mv Pseudomonas aeruginosa
Staphylococcus aureus
RNA sequencing
dual-species biofilms
cystic fibrosis
Science & Technology
topic Pseudomonas aeruginosa
Staphylococcus aureus
RNA sequencing
dual-species biofilms
cystic fibrosis
Science & Technology
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fgene.2022.883199/ full#supplementary-material
publishDate 2022
dc.date.none.fl_str_mv 2022-07-06
2022-07-06T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/79271
url https://hdl.handle.net/1822/79271
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Magalhães, Andreia P.; França, Angela; Pereira, Maria Olívia; Cerca, Nuno, Unveiling co-infection in cystic fibrosis airways: transcriptomic analysis of Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilms. Frontiers in Genetics, 13(883199), 2022
1664-8021
10.3389/fgene.2022.883199
883199
https://www.frontiersin.org/articles/10.3389/fgene.2022.883199
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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