Analysis of the translatome by ribosome profiling in colorectal cancer

Detalhes bibliográficos
Autor(a) principal: Silva, Joana
Data de Publicação: 2015
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/3778
Resumo: PhD in Biology (specialigty: Systems Biology) SFRH/BD/106081/2015
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spelling Analysis of the translatome by ribosome profiling in colorectal cancerGenómica Funcional e EstruturalColorectal CancerPhD in Biology (specialigty: Systems Biology) SFRH/BD/106081/2015Colorectal cancer (CRC) is a serious health problem due to its high incidence and mortality rates despite the major advances in cancer therapeutic approaches [1]. CRC carcinogenesis progression is based in a continuous accumulation of genetic alterations that leads to variations in the overall gene expression profiles [2]. This creates the need for deep analysis of cancer gene expression patterns and, thus, a more reliable understanding of the human proteome to disclose the molecular and cellular pathways as well as the regulatory mechanisms involved in cancer progression [2-4]. Genome wide analyses of gene expression have so far focused on the abundance of mRNA species as measured either by microarray or, more recently, by RNA deep sequencing [5,6]. However, neither approach provides information on protein synthesis, an important end point of gene expression [5,7]. Ribosome profiling is an emerging technique that uses deep sequencing to monitor in vivo translation and provide global and quantitative measurements of translation [7,8]. It can also reveal unexpected complexity in translation, including the presence of ribosomes outside of classical protein-coding regions of the transcriptome [5]. In this approach, translation is profiled by nuclease footprinting of ribosomes on RNA templates and high-throughput sequencing in order to determine the precise positions of ribosomes on a transcript and its overall density [8]. Ribosome profiling studies have been performed in cancer cell lines, where they showed an increase in overall protein identification and new proteins not yet annotated that possibly were originated from N-terminal extensions or upstream open reading frames (uORFs) [9-12]. The main goal of this project is to determine the changes between the translatome of CRC and normal colorectal cells and the role of such alterations in the tumorigenesis process of CRC cells. For this purpose, we will perform ribosome profiling in normal (NCM460) and CRC (HCT116) cell lines. Bioinformatics and gene ontology analysis of the translated mRNAs will elucidate the main cellular pathways through which the corresponding proteins are involved in CRC progression. Then, we will dissect which of these proteins can interfere and induce cell survival of CRC cells. Furthermore, we aim to analyze the potential contribution of translatable short alternative ORFs (AltORFs) and/or the corresponding peptides towards CRC progression. This information will be crucial to the development of new therapeutic strategies for CRC.Romão, LuísaLuchessi, AugustoRepositório Científico do Instituto Nacional de SaúdeSilva, Joana2020-12-31T01:30:12Z2015-122015-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/reportapplication/pdfhttp://hdl.handle.net/10400.18/3778enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:47Zoai:repositorio.insa.pt:10400.18/3778Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:17.914817Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Analysis of the translatome by ribosome profiling in colorectal cancer
title Analysis of the translatome by ribosome profiling in colorectal cancer
spellingShingle Analysis of the translatome by ribosome profiling in colorectal cancer
Silva, Joana
Genómica Funcional e Estrutural
Colorectal Cancer
title_short Analysis of the translatome by ribosome profiling in colorectal cancer
title_full Analysis of the translatome by ribosome profiling in colorectal cancer
title_fullStr Analysis of the translatome by ribosome profiling in colorectal cancer
title_full_unstemmed Analysis of the translatome by ribosome profiling in colorectal cancer
title_sort Analysis of the translatome by ribosome profiling in colorectal cancer
author Silva, Joana
author_facet Silva, Joana
author_role author
dc.contributor.none.fl_str_mv Romão, Luísa
Luchessi, Augusto
Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Silva, Joana
dc.subject.por.fl_str_mv Genómica Funcional e Estrutural
Colorectal Cancer
topic Genómica Funcional e Estrutural
Colorectal Cancer
description PhD in Biology (specialigty: Systems Biology) SFRH/BD/106081/2015
publishDate 2015
dc.date.none.fl_str_mv 2015-12
2015-12-01T00:00:00Z
2020-12-31T01:30:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/3778
url http://hdl.handle.net/10400.18/3778
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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