Skeletal muscle dispersion (400-1000 nm) and kinetics at optical clearing

Detalhes bibliográficos
Autor(a) principal: Oliveira, Luís
Data de Publicação: 2017
Outros Autores: Carvalho, Maria Inês, Nogueira, Elisabete, Tuchin, Valery
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/10333
Resumo: Skeletal muscle dispersion and optical clearing (OC) kinetics were studied experimentally to prove the existence of the refractive index (RI) matching mechanism of OC. Sample thickness and collimated transmittance spectra were measured during treatments with glucose (40%) and ethylene glycol (EG; 99%) solutions and used to obtain the time dependence of the RI of tissue fluids based on the proposed theoretical model. Calculated results demonstrated an increase of RI of tissue fluids and consequently proved the occurrence of the RI matching mechanism. The RI increase was observed for the wavelength range between 400 and 1000 nm and for the 2 probing molecules explored. We found that for 30 min treatment with 40% glucose and 99% EG, RI of sarcoplasm plus interstitial fluid was increased at 800 nm from 1.328 to 1.348 and from 1.328 to 1.369, respectively.
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spelling Skeletal muscle dispersion (400-1000 nm) and kinetics at optical clearingOptical clearingRefractive indexRefractive index matchingSkeletal muscleSkeletal muscle dispersion and optical clearing (OC) kinetics were studied experimentally to prove the existence of the refractive index (RI) matching mechanism of OC. Sample thickness and collimated transmittance spectra were measured during treatments with glucose (40%) and ethylene glycol (EG; 99%) solutions and used to obtain the time dependence of the RI of tissue fluids based on the proposed theoretical model. Calculated results demonstrated an increase of RI of tissue fluids and consequently proved the occurrence of the RI matching mechanism. The RI increase was observed for the wavelength range between 400 and 1000 nm and for the 2 probing molecules explored. We found that for 30 min treatment with 40% glucose and 99% EG, RI of sarcoplasm plus interstitial fluid was increased at 800 nm from 1.328 to 1.348 and from 1.328 to 1.369, respectively.WileyRepositório Científico do Instituto Politécnico do PortoOliveira, LuísCarvalho, Maria InêsNogueira, ElisabeteTuchin, Valery2017-09-262117-01-01T00:00:00Z2017-09-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/10333eng10.1002/jbio.201700094metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:51:52Zoai:recipp.ipp.pt:10400.22/10333Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:30:46.706499Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Skeletal muscle dispersion (400-1000 nm) and kinetics at optical clearing
title Skeletal muscle dispersion (400-1000 nm) and kinetics at optical clearing
spellingShingle Skeletal muscle dispersion (400-1000 nm) and kinetics at optical clearing
Oliveira, Luís
Optical clearing
Refractive index
Refractive index matching
Skeletal muscle
title_short Skeletal muscle dispersion (400-1000 nm) and kinetics at optical clearing
title_full Skeletal muscle dispersion (400-1000 nm) and kinetics at optical clearing
title_fullStr Skeletal muscle dispersion (400-1000 nm) and kinetics at optical clearing
title_full_unstemmed Skeletal muscle dispersion (400-1000 nm) and kinetics at optical clearing
title_sort Skeletal muscle dispersion (400-1000 nm) and kinetics at optical clearing
author Oliveira, Luís
author_facet Oliveira, Luís
Carvalho, Maria Inês
Nogueira, Elisabete
Tuchin, Valery
author_role author
author2 Carvalho, Maria Inês
Nogueira, Elisabete
Tuchin, Valery
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Oliveira, Luís
Carvalho, Maria Inês
Nogueira, Elisabete
Tuchin, Valery
dc.subject.por.fl_str_mv Optical clearing
Refractive index
Refractive index matching
Skeletal muscle
topic Optical clearing
Refractive index
Refractive index matching
Skeletal muscle
description Skeletal muscle dispersion and optical clearing (OC) kinetics were studied experimentally to prove the existence of the refractive index (RI) matching mechanism of OC. Sample thickness and collimated transmittance spectra were measured during treatments with glucose (40%) and ethylene glycol (EG; 99%) solutions and used to obtain the time dependence of the RI of tissue fluids based on the proposed theoretical model. Calculated results demonstrated an increase of RI of tissue fluids and consequently proved the occurrence of the RI matching mechanism. The RI increase was observed for the wavelength range between 400 and 1000 nm and for the 2 probing molecules explored. We found that for 30 min treatment with 40% glucose and 99% EG, RI of sarcoplasm plus interstitial fluid was increased at 800 nm from 1.328 to 1.348 and from 1.328 to 1.369, respectively.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-26
2017-09-26T00:00:00Z
2117-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/10333
url http://hdl.handle.net/10400.22/10333
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1002/jbio.201700094
dc.rights.driver.fl_str_mv metadata only access
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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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