Extraction of ochratoxin A in bread samples by the QuEChERS methodology

Detalhes bibliográficos
Autor(a) principal: Paíga, Paula
Data de Publicação: 2012
Outros Autores: Morais, Simone, Oliva-Teles, Maria Teresa, Correia, Manuela, Delerue-Matos, Cristina, Duarte, S. C., Pena, Angelina, Lino, C. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/2141
Resumo: A QuEChERS method for the extraction of ochratoxin A (OTA) from bread samples was evaluated. A factorial design (23) was used to find the optimal QuEChERS parameters (extraction time, extraction solvent volume and sample mass). Extracts were analysed by LC with fluorescence detection. The optimal extraction conditions were: 5 g of sample, 15 mL of acetonitrile and 3 min of agitation. The extraction procedure was validated by systematic recovery experiments at three levels. The recoveries obtained ranged from 94.8% (at 1.0 μg kg -1) to 96.6% (at 3.0 μg kg -1). The limit of quantification of the method was 0.05 μg kg -1. The optimised procedure was applied to 20 samples of different bread types (‘‘Carcaça’’, ‘‘Broa de Milho’’, and ‘‘Broa de Avintes’’) highly consumed in Portugal. None of the samples exceeded the established European legal limit of 3 μg kg -1.
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spelling Extraction of ochratoxin A in bread samples by the QuEChERS methodologyOchratoxin ABreadQuEChERSFactorial designA QuEChERS method for the extraction of ochratoxin A (OTA) from bread samples was evaluated. A factorial design (23) was used to find the optimal QuEChERS parameters (extraction time, extraction solvent volume and sample mass). Extracts were analysed by LC with fluorescence detection. The optimal extraction conditions were: 5 g of sample, 15 mL of acetonitrile and 3 min of agitation. The extraction procedure was validated by systematic recovery experiments at three levels. The recoveries obtained ranged from 94.8% (at 1.0 μg kg -1) to 96.6% (at 3.0 μg kg -1). The limit of quantification of the method was 0.05 μg kg -1. The optimised procedure was applied to 20 samples of different bread types (‘‘Carcaça’’, ‘‘Broa de Milho’’, and ‘‘Broa de Avintes’’) highly consumed in Portugal. None of the samples exceeded the established European legal limit of 3 μg kg -1.ElsevierRepositório Científico do Instituto Politécnico do PortoPaíga, PaulaMorais, SimoneOliva-Teles, Maria TeresaCorreia, ManuelaDelerue-Matos, CristinaDuarte, S. C.Pena, AngelinaLino, C. M.2013-10-03T09:37:02Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/2141eng0308-814610.1016/j.foodchem.2012.06.045metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:41:37Zoai:recipp.ipp.pt:10400.22/2141Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:23:10.948666Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Extraction of ochratoxin A in bread samples by the QuEChERS methodology
title Extraction of ochratoxin A in bread samples by the QuEChERS methodology
spellingShingle Extraction of ochratoxin A in bread samples by the QuEChERS methodology
Paíga, Paula
Ochratoxin A
Bread
QuEChERS
Factorial design
title_short Extraction of ochratoxin A in bread samples by the QuEChERS methodology
title_full Extraction of ochratoxin A in bread samples by the QuEChERS methodology
title_fullStr Extraction of ochratoxin A in bread samples by the QuEChERS methodology
title_full_unstemmed Extraction of ochratoxin A in bread samples by the QuEChERS methodology
title_sort Extraction of ochratoxin A in bread samples by the QuEChERS methodology
author Paíga, Paula
author_facet Paíga, Paula
Morais, Simone
Oliva-Teles, Maria Teresa
Correia, Manuela
Delerue-Matos, Cristina
Duarte, S. C.
Pena, Angelina
Lino, C. M.
author_role author
author2 Morais, Simone
Oliva-Teles, Maria Teresa
Correia, Manuela
Delerue-Matos, Cristina
Duarte, S. C.
Pena, Angelina
Lino, C. M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Paíga, Paula
Morais, Simone
Oliva-Teles, Maria Teresa
Correia, Manuela
Delerue-Matos, Cristina
Duarte, S. C.
Pena, Angelina
Lino, C. M.
dc.subject.por.fl_str_mv Ochratoxin A
Bread
QuEChERS
Factorial design
topic Ochratoxin A
Bread
QuEChERS
Factorial design
description A QuEChERS method for the extraction of ochratoxin A (OTA) from bread samples was evaluated. A factorial design (23) was used to find the optimal QuEChERS parameters (extraction time, extraction solvent volume and sample mass). Extracts were analysed by LC with fluorescence detection. The optimal extraction conditions were: 5 g of sample, 15 mL of acetonitrile and 3 min of agitation. The extraction procedure was validated by systematic recovery experiments at three levels. The recoveries obtained ranged from 94.8% (at 1.0 μg kg -1) to 96.6% (at 3.0 μg kg -1). The limit of quantification of the method was 0.05 μg kg -1. The optimised procedure was applied to 20 samples of different bread types (‘‘Carcaça’’, ‘‘Broa de Milho’’, and ‘‘Broa de Avintes’’) highly consumed in Portugal. None of the samples exceeded the established European legal limit of 3 μg kg -1.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2013-10-03T09:37:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/2141
url http://hdl.handle.net/10400.22/2141
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0308-8146
10.1016/j.foodchem.2012.06.045
dc.rights.driver.fl_str_mv metadata only access
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rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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