Production of membrane histidine kinases and structural studies on Thiosulfate dehydrogenase

Detalhes bibliográficos
Autor(a) principal: Kulakova, Alina Vitaliyivna
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/43578
Resumo: This thesis is organized in three chapters. Chapter 1 comprises a brief introduction on the methodology used throughout the work herein presented. Chapter 2 describes the production of membrane histidine kinases from Staphylococcus aureus and Clostridium difficile and chapter 3 the structural studies of a thiosulfate dehydrogenase from Campylobacter jejuni. The experimental work was performed in the Membrane Protein Crystallography Laboratory at Instituto de Tecnologia Química e Biológica, and also included a visit to SOLEIL synchrotron, Paris, France, to collect X-ray diffraction data.
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spelling Production of membrane histidine kinases and structural studies on Thiosulfate dehydrogenaseHistidine kinasesmembrane protein production and purificationX-ray crystallographythiosulfate dehydrogenaseBiological SciencesBiochemistry for HealthThis thesis is organized in three chapters. Chapter 1 comprises a brief introduction on the methodology used throughout the work herein presented. Chapter 2 describes the production of membrane histidine kinases from Staphylococcus aureus and Clostridium difficile and chapter 3 the structural studies of a thiosulfate dehydrogenase from Campylobacter jejuni. The experimental work was performed in the Membrane Protein Crystallography Laboratory at Instituto de Tecnologia Química e Biológica, and also included a visit to SOLEIL synchrotron, Paris, France, to collect X-ray diffraction data.Archer, MargaridaRosário, Ana LúciaBrito, JoséRUNKulakova, Alina Vitaliyivna2018-08-07T15:52:55Z2015-12-092015-102015-12-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/43578TID:202011950enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:23:40Zoai:run.unl.pt:10362/43578Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:31:43.822884Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Production of membrane histidine kinases and structural studies on Thiosulfate dehydrogenase
title Production of membrane histidine kinases and structural studies on Thiosulfate dehydrogenase
spellingShingle Production of membrane histidine kinases and structural studies on Thiosulfate dehydrogenase
Kulakova, Alina Vitaliyivna
Histidine kinases
membrane protein production and purification
X-ray crystallography
thiosulfate dehydrogenase
Biological Sciences
Biochemistry for Health
title_short Production of membrane histidine kinases and structural studies on Thiosulfate dehydrogenase
title_full Production of membrane histidine kinases and structural studies on Thiosulfate dehydrogenase
title_fullStr Production of membrane histidine kinases and structural studies on Thiosulfate dehydrogenase
title_full_unstemmed Production of membrane histidine kinases and structural studies on Thiosulfate dehydrogenase
title_sort Production of membrane histidine kinases and structural studies on Thiosulfate dehydrogenase
author Kulakova, Alina Vitaliyivna
author_facet Kulakova, Alina Vitaliyivna
author_role author
dc.contributor.none.fl_str_mv Archer, Margarida
Rosário, Ana Lúcia
Brito, José
RUN
dc.contributor.author.fl_str_mv Kulakova, Alina Vitaliyivna
dc.subject.por.fl_str_mv Histidine kinases
membrane protein production and purification
X-ray crystallography
thiosulfate dehydrogenase
Biological Sciences
Biochemistry for Health
topic Histidine kinases
membrane protein production and purification
X-ray crystallography
thiosulfate dehydrogenase
Biological Sciences
Biochemistry for Health
description This thesis is organized in three chapters. Chapter 1 comprises a brief introduction on the methodology used throughout the work herein presented. Chapter 2 describes the production of membrane histidine kinases from Staphylococcus aureus and Clostridium difficile and chapter 3 the structural studies of a thiosulfate dehydrogenase from Campylobacter jejuni. The experimental work was performed in the Membrane Protein Crystallography Laboratory at Instituto de Tecnologia Química e Biológica, and also included a visit to SOLEIL synchrotron, Paris, France, to collect X-ray diffraction data.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-09
2015-10
2015-12-09T00:00:00Z
2018-08-07T15:52:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/43578
TID:202011950
url http://hdl.handle.net/10362/43578
identifier_str_mv TID:202011950
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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