MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/62326 |
Resumo: | Background: Laboratory practice commonly implies rodent anesthesia. Such is instrumental not only for animal welfare, but also for standardized assessment of new anesthetics. New method: We developed and validated a grading system for a temporal follow-up of anesthesia. The Minho Objective Rodent Phenotypical Anesthesia (MORPhA) scale was tested in mice (C57BL/6 J) and rats (WistarHan) anesthetized with ketamine/dexmedetomidine (ket/dex). The scale comprises 12 behavioral readouts organized in 5 stages – (i) normal/(ii) hindered voluntary movement, elicited response to (iii) non-noxious/(iv) noxious stimuli and (v) absence of response – evaluated at regular time-points. Progression across stages was monitored by electroencephalography (EEG) in rats during anesthesia induction and reversal (atipamezole) and during induction with a second anesthetic drug (pentobarbital). Results: Higher anesthetic doses decreased the time to reach higher levels of anesthesia during progression, while increasing the time to regain waking behavior during reversal in both mice and rats. A regular decrease in high frequencies (low and high gamma) power was observed as the MORPhA score increased during anesthesia induction, while the opposite pattern was observed during emergence from anesthesia through reversion of dex effect. Comparison with existing methods: Degree of anesthesia in laboratory rodents is normally evaluated by testing loss of reflexes. While these are useful endpoint assessments, they are of limited application to study induction/ reversal kinetics or factors affecting individual susceptibility. MORPhA allows graded standardized assessment of this progression in a biologically-relevant fashion. Conclusions: The devised anesthetic scale is of simple application and provides a semi-quantifiable readout of anesthesia induction/reversal. |
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MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scaleBehavioral scaleAnesthesiaEEGKetamine/dexmedetomidinePentobarbitalAnesthesia inductionAnesthesia reversalScience & TechnologyBackground: Laboratory practice commonly implies rodent anesthesia. Such is instrumental not only for animal welfare, but also for standardized assessment of new anesthetics. New method: We developed and validated a grading system for a temporal follow-up of anesthesia. The Minho Objective Rodent Phenotypical Anesthesia (MORPhA) scale was tested in mice (C57BL/6 J) and rats (WistarHan) anesthetized with ketamine/dexmedetomidine (ket/dex). The scale comprises 12 behavioral readouts organized in 5 stages – (i) normal/(ii) hindered voluntary movement, elicited response to (iii) non-noxious/(iv) noxious stimuli and (v) absence of response – evaluated at regular time-points. Progression across stages was monitored by electroencephalography (EEG) in rats during anesthesia induction and reversal (atipamezole) and during induction with a second anesthetic drug (pentobarbital). Results: Higher anesthetic doses decreased the time to reach higher levels of anesthesia during progression, while increasing the time to regain waking behavior during reversal in both mice and rats. A regular decrease in high frequencies (low and high gamma) power was observed as the MORPhA score increased during anesthesia induction, while the opposite pattern was observed during emergence from anesthesia through reversion of dex effect. Comparison with existing methods: Degree of anesthesia in laboratory rodents is normally evaluated by testing loss of reflexes. While these are useful endpoint assessments, they are of limited application to study induction/ reversal kinetics or factors affecting individual susceptibility. MORPhA allows graded standardized assessment of this progression in a biologically-relevant fashion. Conclusions: The devised anesthetic scale is of simple application and provides a semi-quantifiable readout of anesthesia induction/reversal.This work was supported by FEDER funds, through the Competitiveness Factors Operational Programme (COMPETE), and by National funds, through the Foundation for Science and Technology (FCT) [projects POCI-01-0145-FEDER-007038 and PTDC/NEU-SCC/ 5301/2014]. Researchers were supported by FCT [grant numbers SFRH/BD/52291/2013 to ME via Inter-University Doctoral Programme in Ageing and Chronic Disease, PhDOC; PDE/BDE/113601/2015 to PSM via PhD Program in Health Sciences (Applied), Phd-iHES; SFRH/ BPD/80118/2011 to HLA; SFRH/BD/88932/2012 to JS and IF/01799/ 2013 to IS].ElsevierUniversidade do MinhoEsteves, MadalenaAlmeida, António M.Silva, Joana Margarida Gonçalves MotaMoreira, Pedro Miguel SilvaCarvalho, EmanuelPêgo, José M.Almeida, ArmandoSotiropoulos, I.Sousa, NunoLeite-Almeida, Hugo2019-08-012024-08-01T00:00:00Z2019-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/62326eng0165-02701872-678X10.1016/j.jneumeth.2019.05.01331141704info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:59:05Zoai:repositorium.sdum.uminho.pt:1822/62326Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:48:49.695245Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale |
title |
MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale |
spellingShingle |
MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale Esteves, Madalena Behavioral scale Anesthesia EEG Ketamine/dexmedetomidine Pentobarbital Anesthesia induction Anesthesia reversal Science & Technology |
title_short |
MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale |
title_full |
MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale |
title_fullStr |
MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale |
title_full_unstemmed |
MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale |
title_sort |
MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale |
author |
Esteves, Madalena |
author_facet |
Esteves, Madalena Almeida, António M. Silva, Joana Margarida Gonçalves Mota Moreira, Pedro Miguel Silva Carvalho, Emanuel Pêgo, José M. Almeida, Armando Sotiropoulos, I. Sousa, Nuno Leite-Almeida, Hugo |
author_role |
author |
author2 |
Almeida, António M. Silva, Joana Margarida Gonçalves Mota Moreira, Pedro Miguel Silva Carvalho, Emanuel Pêgo, José M. Almeida, Armando Sotiropoulos, I. Sousa, Nuno Leite-Almeida, Hugo |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Esteves, Madalena Almeida, António M. Silva, Joana Margarida Gonçalves Mota Moreira, Pedro Miguel Silva Carvalho, Emanuel Pêgo, José M. Almeida, Armando Sotiropoulos, I. Sousa, Nuno Leite-Almeida, Hugo |
dc.subject.por.fl_str_mv |
Behavioral scale Anesthesia EEG Ketamine/dexmedetomidine Pentobarbital Anesthesia induction Anesthesia reversal Science & Technology |
topic |
Behavioral scale Anesthesia EEG Ketamine/dexmedetomidine Pentobarbital Anesthesia induction Anesthesia reversal Science & Technology |
description |
Background: Laboratory practice commonly implies rodent anesthesia. Such is instrumental not only for animal welfare, but also for standardized assessment of new anesthetics. New method: We developed and validated a grading system for a temporal follow-up of anesthesia. The Minho Objective Rodent Phenotypical Anesthesia (MORPhA) scale was tested in mice (C57BL/6 J) and rats (WistarHan) anesthetized with ketamine/dexmedetomidine (ket/dex). The scale comprises 12 behavioral readouts organized in 5 stages – (i) normal/(ii) hindered voluntary movement, elicited response to (iii) non-noxious/(iv) noxious stimuli and (v) absence of response – evaluated at regular time-points. Progression across stages was monitored by electroencephalography (EEG) in rats during anesthesia induction and reversal (atipamezole) and during induction with a second anesthetic drug (pentobarbital). Results: Higher anesthetic doses decreased the time to reach higher levels of anesthesia during progression, while increasing the time to regain waking behavior during reversal in both mice and rats. A regular decrease in high frequencies (low and high gamma) power was observed as the MORPhA score increased during anesthesia induction, while the opposite pattern was observed during emergence from anesthesia through reversion of dex effect. Comparison with existing methods: Degree of anesthesia in laboratory rodents is normally evaluated by testing loss of reflexes. While these are useful endpoint assessments, they are of limited application to study induction/ reversal kinetics or factors affecting individual susceptibility. MORPhA allows graded standardized assessment of this progression in a biologically-relevant fashion. Conclusions: The devised anesthetic scale is of simple application and provides a semi-quantifiable readout of anesthesia induction/reversal. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-01 2019-08-01T00:00:00Z 2024-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/62326 |
url |
http://hdl.handle.net/1822/62326 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0165-0270 1872-678X 10.1016/j.jneumeth.2019.05.013 31141704 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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