MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale

Detalhes bibliográficos
Autor(a) principal: Esteves, Madalena
Data de Publicação: 2019
Outros Autores: Almeida, António M., Silva, Joana Margarida Gonçalves Mota, Moreira, Pedro Miguel Silva, Carvalho, Emanuel, Pêgo, José M., Almeida, Armando, Sotiropoulos, I., Sousa, Nuno, Leite-Almeida, Hugo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/62326
Resumo: Background: Laboratory practice commonly implies rodent anesthesia. Such is instrumental not only for animal welfare, but also for standardized assessment of new anesthetics. New method: We developed and validated a grading system for a temporal follow-up of anesthesia. The Minho Objective Rodent Phenotypical Anesthesia (MORPhA) scale was tested in mice (C57BL/6 J) and rats (WistarHan) anesthetized with ketamine/dexmedetomidine (ket/dex). The scale comprises 12 behavioral readouts organized in 5 stages – (i) normal/(ii) hindered voluntary movement, elicited response to (iii) non-noxious/(iv) noxious stimuli and (v) absence of response – evaluated at regular time-points. Progression across stages was monitored by electroencephalography (EEG) in rats during anesthesia induction and reversal (atipamezole) and during induction with a second anesthetic drug (pentobarbital). Results: Higher anesthetic doses decreased the time to reach higher levels of anesthesia during progression, while increasing the time to regain waking behavior during reversal in both mice and rats. A regular decrease in high frequencies (low and high gamma) power was observed as the MORPhA score increased during anesthesia induction, while the opposite pattern was observed during emergence from anesthesia through reversion of dex effect. Comparison with existing methods: Degree of anesthesia in laboratory rodents is normally evaluated by testing loss of reflexes. While these are useful endpoint assessments, they are of limited application to study induction/ reversal kinetics or factors affecting individual susceptibility. MORPhA allows graded standardized assessment of this progression in a biologically-relevant fashion. Conclusions: The devised anesthetic scale is of simple application and provides a semi-quantifiable readout of anesthesia induction/reversal.
id RCAP_9c280cc80b3e5001728487f2081a4490
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/62326
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scaleBehavioral scaleAnesthesiaEEGKetamine/dexmedetomidinePentobarbitalAnesthesia inductionAnesthesia reversalScience & TechnologyBackground: Laboratory practice commonly implies rodent anesthesia. Such is instrumental not only for animal welfare, but also for standardized assessment of new anesthetics. New method: We developed and validated a grading system for a temporal follow-up of anesthesia. The Minho Objective Rodent Phenotypical Anesthesia (MORPhA) scale was tested in mice (C57BL/6 J) and rats (WistarHan) anesthetized with ketamine/dexmedetomidine (ket/dex). The scale comprises 12 behavioral readouts organized in 5 stages – (i) normal/(ii) hindered voluntary movement, elicited response to (iii) non-noxious/(iv) noxious stimuli and (v) absence of response – evaluated at regular time-points. Progression across stages was monitored by electroencephalography (EEG) in rats during anesthesia induction and reversal (atipamezole) and during induction with a second anesthetic drug (pentobarbital). Results: Higher anesthetic doses decreased the time to reach higher levels of anesthesia during progression, while increasing the time to regain waking behavior during reversal in both mice and rats. A regular decrease in high frequencies (low and high gamma) power was observed as the MORPhA score increased during anesthesia induction, while the opposite pattern was observed during emergence from anesthesia through reversion of dex effect. Comparison with existing methods: Degree of anesthesia in laboratory rodents is normally evaluated by testing loss of reflexes. While these are useful endpoint assessments, they are of limited application to study induction/ reversal kinetics or factors affecting individual susceptibility. MORPhA allows graded standardized assessment of this progression in a biologically-relevant fashion. Conclusions: The devised anesthetic scale is of simple application and provides a semi-quantifiable readout of anesthesia induction/reversal.This work was supported by FEDER funds, through the Competitiveness Factors Operational Programme (COMPETE), and by National funds, through the Foundation for Science and Technology (FCT) [projects POCI-01-0145-FEDER-007038 and PTDC/NEU-SCC/ 5301/2014]. Researchers were supported by FCT [grant numbers SFRH/BD/52291/2013 to ME via Inter-University Doctoral Programme in Ageing and Chronic Disease, PhDOC; PDE/BDE/113601/2015 to PSM via PhD Program in Health Sciences (Applied), Phd-iHES; SFRH/ BPD/80118/2011 to HLA; SFRH/BD/88932/2012 to JS and IF/01799/ 2013 to IS].ElsevierUniversidade do MinhoEsteves, MadalenaAlmeida, António M.Silva, Joana Margarida Gonçalves MotaMoreira, Pedro Miguel SilvaCarvalho, EmanuelPêgo, José M.Almeida, ArmandoSotiropoulos, I.Sousa, NunoLeite-Almeida, Hugo2019-08-012024-08-01T00:00:00Z2019-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/62326eng0165-02701872-678X10.1016/j.jneumeth.2019.05.01331141704info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:59:05Zoai:repositorium.sdum.uminho.pt:1822/62326Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:48:49.695245Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale
title MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale
spellingShingle MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale
Esteves, Madalena
Behavioral scale
Anesthesia
EEG
Ketamine/dexmedetomidine
Pentobarbital
Anesthesia induction
Anesthesia reversal
Science & Technology
title_short MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale
title_full MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale
title_fullStr MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale
title_full_unstemmed MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale
title_sort MORPhA Scale: behavioral and electroencephalographic validation of a rodent anesthesia scale
author Esteves, Madalena
author_facet Esteves, Madalena
Almeida, António M.
Silva, Joana Margarida Gonçalves Mota
Moreira, Pedro Miguel Silva
Carvalho, Emanuel
Pêgo, José M.
Almeida, Armando
Sotiropoulos, I.
Sousa, Nuno
Leite-Almeida, Hugo
author_role author
author2 Almeida, António M.
Silva, Joana Margarida Gonçalves Mota
Moreira, Pedro Miguel Silva
Carvalho, Emanuel
Pêgo, José M.
Almeida, Armando
Sotiropoulos, I.
Sousa, Nuno
Leite-Almeida, Hugo
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Esteves, Madalena
Almeida, António M.
Silva, Joana Margarida Gonçalves Mota
Moreira, Pedro Miguel Silva
Carvalho, Emanuel
Pêgo, José M.
Almeida, Armando
Sotiropoulos, I.
Sousa, Nuno
Leite-Almeida, Hugo
dc.subject.por.fl_str_mv Behavioral scale
Anesthesia
EEG
Ketamine/dexmedetomidine
Pentobarbital
Anesthesia induction
Anesthesia reversal
Science & Technology
topic Behavioral scale
Anesthesia
EEG
Ketamine/dexmedetomidine
Pentobarbital
Anesthesia induction
Anesthesia reversal
Science & Technology
description Background: Laboratory practice commonly implies rodent anesthesia. Such is instrumental not only for animal welfare, but also for standardized assessment of new anesthetics. New method: We developed and validated a grading system for a temporal follow-up of anesthesia. The Minho Objective Rodent Phenotypical Anesthesia (MORPhA) scale was tested in mice (C57BL/6 J) and rats (WistarHan) anesthetized with ketamine/dexmedetomidine (ket/dex). The scale comprises 12 behavioral readouts organized in 5 stages – (i) normal/(ii) hindered voluntary movement, elicited response to (iii) non-noxious/(iv) noxious stimuli and (v) absence of response – evaluated at regular time-points. Progression across stages was monitored by electroencephalography (EEG) in rats during anesthesia induction and reversal (atipamezole) and during induction with a second anesthetic drug (pentobarbital). Results: Higher anesthetic doses decreased the time to reach higher levels of anesthesia during progression, while increasing the time to regain waking behavior during reversal in both mice and rats. A regular decrease in high frequencies (low and high gamma) power was observed as the MORPhA score increased during anesthesia induction, while the opposite pattern was observed during emergence from anesthesia through reversion of dex effect. Comparison with existing methods: Degree of anesthesia in laboratory rodents is normally evaluated by testing loss of reflexes. While these are useful endpoint assessments, they are of limited application to study induction/ reversal kinetics or factors affecting individual susceptibility. MORPhA allows graded standardized assessment of this progression in a biologically-relevant fashion. Conclusions: The devised anesthetic scale is of simple application and provides a semi-quantifiable readout of anesthesia induction/reversal.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-01
2019-08-01T00:00:00Z
2024-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/62326
url http://hdl.handle.net/1822/62326
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0165-0270
1872-678X
10.1016/j.jneumeth.2019.05.013
31141704
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132251100807168