Pontastacus leptodactylus (Eschscholtz, 1823) and Faxonius limosus (Rafinesque, 1817) as new, alternative sources of chitin and chitosan
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/19980 |
Resumo: | The growing demand for chitin and chitosan makes it necessary to look for new sources of these polymers and to develop more environmentally friendly methods for their isolation. The subjects of the current study were chitin and chitosan extracted from shells of two crayfish species: <i>P. leptodactylus</i> and <i>F. limosus</i>. The obtained polymers were characterized by physicochemical properties (molecular weight, thermal stability, and structure). The obtained chitosan was evaluated regarding biocompatibility and antimicrobial activity. The yield of chitin obtained from <i>P. leptodactylus</i> and <i>F. limosus</i> with a standard method was 22 ± 2.7% and 20 ± 3.6% (<i>w</i>/<i>w</i>), respectively (a preliminary extraction with a natural deep eutectic solvent was performed successfully only for <i>P. leptodactylus</i>). The yield of chitosan production was 15 ± 0.3% and 14 ± 4.2%, respectively. Both chitosan samples showed antimicrobial activity against <i>E. coli</i> and <i>S. aureus</i>. Cytotoxicity assays revealed a time- and concentration-dependent effect, with a milder impact at concentrations up to 250 µg/mL. A more favourable profile was observed for chitosan from <i>F. limosus</i> shells. |
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Pontastacus leptodactylus (Eschscholtz, 1823) and Faxonius limosus (Rafinesque, 1817) as new, alternative sources of chitin and chitosanChitinChitosanAntibacterial activityCytotoxicityNADESThe growing demand for chitin and chitosan makes it necessary to look for new sources of these polymers and to develop more environmentally friendly methods for their isolation. The subjects of the current study were chitin and chitosan extracted from shells of two crayfish species: <i>P. leptodactylus</i> and <i>F. limosus</i>. The obtained polymers were characterized by physicochemical properties (molecular weight, thermal stability, and structure). The obtained chitosan was evaluated regarding biocompatibility and antimicrobial activity. The yield of chitin obtained from <i>P. leptodactylus</i> and <i>F. limosus</i> with a standard method was 22 ± 2.7% and 20 ± 3.6% (<i>w</i>/<i>w</i>), respectively (a preliminary extraction with a natural deep eutectic solvent was performed successfully only for <i>P. leptodactylus</i>). The yield of chitosan production was 15 ± 0.3% and 14 ± 4.2%, respectively. Both chitosan samples showed antimicrobial activity against <i>E. coli</i> and <i>S. aureus</i>. Cytotoxicity assays revealed a time- and concentration-dependent effect, with a milder impact at concentrations up to 250 µg/mL. A more favourable profile was observed for chitosan from <i>F. limosus</i> shells.MDPISapientiaNuc, ZofiaBrusotti, GloriaCatenacci, LauraGrenha, AnaPontes, Jorge FilipePinto-da-Silva, JoanaRosa Da Costa, AnaMoro, PaolaMilanese, ChiaraGrisoli, PietroSorrenti, MilenaDobrzycka-Krahel, AldonaBonferoni, Maria CristinaCaramella, Carla Marcella2023-09-13T13:36:22Z2023-08-222023-09-08T12:44:43Z2023-08-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/19980engWater 15 (17): 3024 (2023)2073-444110.3390/w15173024info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-20T02:00:33Zoai:sapientia.ualg.pt:10400.1/19980Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:29:43.127313Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Pontastacus leptodactylus (Eschscholtz, 1823) and Faxonius limosus (Rafinesque, 1817) as new, alternative sources of chitin and chitosan |
title |
Pontastacus leptodactylus (Eschscholtz, 1823) and Faxonius limosus (Rafinesque, 1817) as new, alternative sources of chitin and chitosan |
spellingShingle |
Pontastacus leptodactylus (Eschscholtz, 1823) and Faxonius limosus (Rafinesque, 1817) as new, alternative sources of chitin and chitosan Nuc, Zofia Chitin Chitosan Antibacterial activity Cytotoxicity NADES |
title_short |
Pontastacus leptodactylus (Eschscholtz, 1823) and Faxonius limosus (Rafinesque, 1817) as new, alternative sources of chitin and chitosan |
title_full |
Pontastacus leptodactylus (Eschscholtz, 1823) and Faxonius limosus (Rafinesque, 1817) as new, alternative sources of chitin and chitosan |
title_fullStr |
Pontastacus leptodactylus (Eschscholtz, 1823) and Faxonius limosus (Rafinesque, 1817) as new, alternative sources of chitin and chitosan |
title_full_unstemmed |
Pontastacus leptodactylus (Eschscholtz, 1823) and Faxonius limosus (Rafinesque, 1817) as new, alternative sources of chitin and chitosan |
title_sort |
Pontastacus leptodactylus (Eschscholtz, 1823) and Faxonius limosus (Rafinesque, 1817) as new, alternative sources of chitin and chitosan |
author |
Nuc, Zofia |
author_facet |
Nuc, Zofia Brusotti, Gloria Catenacci, Laura Grenha, Ana Pontes, Jorge Filipe Pinto-da-Silva, Joana Rosa Da Costa, Ana Moro, Paola Milanese, Chiara Grisoli, Pietro Sorrenti, Milena Dobrzycka-Krahel, Aldona Bonferoni, Maria Cristina Caramella, Carla Marcella |
author_role |
author |
author2 |
Brusotti, Gloria Catenacci, Laura Grenha, Ana Pontes, Jorge Filipe Pinto-da-Silva, Joana Rosa Da Costa, Ana Moro, Paola Milanese, Chiara Grisoli, Pietro Sorrenti, Milena Dobrzycka-Krahel, Aldona Bonferoni, Maria Cristina Caramella, Carla Marcella |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Nuc, Zofia Brusotti, Gloria Catenacci, Laura Grenha, Ana Pontes, Jorge Filipe Pinto-da-Silva, Joana Rosa Da Costa, Ana Moro, Paola Milanese, Chiara Grisoli, Pietro Sorrenti, Milena Dobrzycka-Krahel, Aldona Bonferoni, Maria Cristina Caramella, Carla Marcella |
dc.subject.por.fl_str_mv |
Chitin Chitosan Antibacterial activity Cytotoxicity NADES |
topic |
Chitin Chitosan Antibacterial activity Cytotoxicity NADES |
description |
The growing demand for chitin and chitosan makes it necessary to look for new sources of these polymers and to develop more environmentally friendly methods for their isolation. The subjects of the current study were chitin and chitosan extracted from shells of two crayfish species: <i>P. leptodactylus</i> and <i>F. limosus</i>. The obtained polymers were characterized by physicochemical properties (molecular weight, thermal stability, and structure). The obtained chitosan was evaluated regarding biocompatibility and antimicrobial activity. The yield of chitin obtained from <i>P. leptodactylus</i> and <i>F. limosus</i> with a standard method was 22 ± 2.7% and 20 ± 3.6% (<i>w</i>/<i>w</i>), respectively (a preliminary extraction with a natural deep eutectic solvent was performed successfully only for <i>P. leptodactylus</i>). The yield of chitosan production was 15 ± 0.3% and 14 ± 4.2%, respectively. Both chitosan samples showed antimicrobial activity against <i>E. coli</i> and <i>S. aureus</i>. Cytotoxicity assays revealed a time- and concentration-dependent effect, with a milder impact at concentrations up to 250 µg/mL. A more favourable profile was observed for chitosan from <i>F. limosus</i> shells. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-13T13:36:22Z 2023-08-22 2023-09-08T12:44:43Z 2023-08-22T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/19980 |
url |
http://hdl.handle.net/10400.1/19980 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Water 15 (17): 3024 (2023) 2073-4441 10.3390/w15173024 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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