Exploiting potential probiotic lactic acid bacteria isolated from Chlorella vulgaris photobioreactors as promising vitamin B12 producers
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/42468 |
Resumo: | Lactic acid bacteria (LAB) have been documented as potential vitamin B12 producers and may constitute an exogenous source of cobalamin for the microalga Chlorella vulgaris, which has been described as being able to perform vitamin uptake. Hence, there is an interest in discovering novel B12-producing probiotic LAB. Therefore, the purpose of the current work was to perform a phenotype–genotype analysis of the vitamin B12 biosynthesis capacity of LAB isolated from C. vulgaris bioreactors, and investigate their probiotic potential. Among the selected strains, Lactococcus lactis E32, Levilactobacillus brevis G31, and Pediococcus pentosaceus L51 demonstrated vitamin B12 biosynthesis capacity, with the latter producing the highest (28.19 ± 2.27 pg mL−1). The genomic analysis confirmed the presence of pivotal genes involved in different steps of the biosynthetic pathway (hemL, cbiT, cobC, and cobD). Notably, P. pentosaceus L51 was the only strain harboring cobA, pduU, and pduV genes, which may provide evidence for the presence of the cobalamin operon. All strains demonstrated the capability to withstand harsh gastrointestinal conditions, although P. pentosaceus L51 was more resilient. The potential for de novo cobalamin biosynthesis and remarkable probiotic features highlighted that P. pentosaceus L51 may be considered the most promising candidate strain for developing high-content vitamin B12 formulations. |
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Exploiting potential probiotic lactic acid bacteria isolated from Chlorella vulgaris photobioreactors as promising vitamin B12 producersB12 biosynthesisB12-producing probiotic lactic acid bacteriaC. vulgarisCobalaminLactic acid bacteria (LAB) have been documented as potential vitamin B12 producers and may constitute an exogenous source of cobalamin for the microalga Chlorella vulgaris, which has been described as being able to perform vitamin uptake. Hence, there is an interest in discovering novel B12-producing probiotic LAB. Therefore, the purpose of the current work was to perform a phenotype–genotype analysis of the vitamin B12 biosynthesis capacity of LAB isolated from C. vulgaris bioreactors, and investigate their probiotic potential. Among the selected strains, Lactococcus lactis E32, Levilactobacillus brevis G31, and Pediococcus pentosaceus L51 demonstrated vitamin B12 biosynthesis capacity, with the latter producing the highest (28.19 ± 2.27 pg mL−1). The genomic analysis confirmed the presence of pivotal genes involved in different steps of the biosynthetic pathway (hemL, cbiT, cobC, and cobD). Notably, P. pentosaceus L51 was the only strain harboring cobA, pduU, and pduV genes, which may provide evidence for the presence of the cobalamin operon. All strains demonstrated the capability to withstand harsh gastrointestinal conditions, although P. pentosaceus L51 was more resilient. The potential for de novo cobalamin biosynthesis and remarkable probiotic features highlighted that P. pentosaceus L51 may be considered the most promising candidate strain for developing high-content vitamin B12 formulations.Veritati - Repositório Institucional da Universidade Católica PortuguesaRibeiro, MónicaMaciel, CláudiaCruz, PedroDarmancier, HelenaNogueira, TeresaCosta, MargaridaLaranjeira, JoanaMorais, Rui M. S. C.Teixeira, Paula2023-09-19T16:11:28Z2023-092023-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/42468eng2304-815810.3390/foods1217327785170387507PMC1048696537685210001144800100001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-13T01:33:36Zoai:repositorio.ucp.pt:10400.14/42468Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:30:55.534625Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Exploiting potential probiotic lactic acid bacteria isolated from Chlorella vulgaris photobioreactors as promising vitamin B12 producers |
title |
Exploiting potential probiotic lactic acid bacteria isolated from Chlorella vulgaris photobioreactors as promising vitamin B12 producers |
spellingShingle |
Exploiting potential probiotic lactic acid bacteria isolated from Chlorella vulgaris photobioreactors as promising vitamin B12 producers Ribeiro, Mónica B12 biosynthesis B12-producing probiotic lactic acid bacteria C. vulgaris Cobalamin |
title_short |
Exploiting potential probiotic lactic acid bacteria isolated from Chlorella vulgaris photobioreactors as promising vitamin B12 producers |
title_full |
Exploiting potential probiotic lactic acid bacteria isolated from Chlorella vulgaris photobioreactors as promising vitamin B12 producers |
title_fullStr |
Exploiting potential probiotic lactic acid bacteria isolated from Chlorella vulgaris photobioreactors as promising vitamin B12 producers |
title_full_unstemmed |
Exploiting potential probiotic lactic acid bacteria isolated from Chlorella vulgaris photobioreactors as promising vitamin B12 producers |
title_sort |
Exploiting potential probiotic lactic acid bacteria isolated from Chlorella vulgaris photobioreactors as promising vitamin B12 producers |
author |
Ribeiro, Mónica |
author_facet |
Ribeiro, Mónica Maciel, Cláudia Cruz, Pedro Darmancier, Helena Nogueira, Teresa Costa, Margarida Laranjeira, Joana Morais, Rui M. S. C. Teixeira, Paula |
author_role |
author |
author2 |
Maciel, Cláudia Cruz, Pedro Darmancier, Helena Nogueira, Teresa Costa, Margarida Laranjeira, Joana Morais, Rui M. S. C. Teixeira, Paula |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Ribeiro, Mónica Maciel, Cláudia Cruz, Pedro Darmancier, Helena Nogueira, Teresa Costa, Margarida Laranjeira, Joana Morais, Rui M. S. C. Teixeira, Paula |
dc.subject.por.fl_str_mv |
B12 biosynthesis B12-producing probiotic lactic acid bacteria C. vulgaris Cobalamin |
topic |
B12 biosynthesis B12-producing probiotic lactic acid bacteria C. vulgaris Cobalamin |
description |
Lactic acid bacteria (LAB) have been documented as potential vitamin B12 producers and may constitute an exogenous source of cobalamin for the microalga Chlorella vulgaris, which has been described as being able to perform vitamin uptake. Hence, there is an interest in discovering novel B12-producing probiotic LAB. Therefore, the purpose of the current work was to perform a phenotype–genotype analysis of the vitamin B12 biosynthesis capacity of LAB isolated from C. vulgaris bioreactors, and investigate their probiotic potential. Among the selected strains, Lactococcus lactis E32, Levilactobacillus brevis G31, and Pediococcus pentosaceus L51 demonstrated vitamin B12 biosynthesis capacity, with the latter producing the highest (28.19 ± 2.27 pg mL−1). The genomic analysis confirmed the presence of pivotal genes involved in different steps of the biosynthetic pathway (hemL, cbiT, cobC, and cobD). Notably, P. pentosaceus L51 was the only strain harboring cobA, pduU, and pduV genes, which may provide evidence for the presence of the cobalamin operon. All strains demonstrated the capability to withstand harsh gastrointestinal conditions, although P. pentosaceus L51 was more resilient. The potential for de novo cobalamin biosynthesis and remarkable probiotic features highlighted that P. pentosaceus L51 may be considered the most promising candidate strain for developing high-content vitamin B12 formulations. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-19T16:11:28Z 2023-09 2023-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/42468 |
url |
http://hdl.handle.net/10400.14/42468 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2304-8158 10.3390/foods12173277 85170387507 PMC10486965 37685210 001144800100001 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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