The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity

Detalhes bibliográficos
Autor(a) principal: Monteiro, João M.
Data de Publicação: 2019
Outros Autores: Covas, Gonçalo, Rausch, Daniela, Filipe, Sérgio R., Schneider, Tanja, Sahl, Hans Georg, Pinho, Mariana G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1038/s41598-019-41461-1
Resumo: This study was funded by the European Research Council through grant ERC-2017-CoG 771709 (to M.G.P.), by Project LISBOA-01-0145-FEDER-007660 Microbiologia Molecular, Estrutural e Celular (to ITQB-NOVA), by the German Research Foundation (DFG; SCHN1284/1-2) to T.S. and FCT fellowship SFRH/BD/71993/2010 (J.M.M.). Mass spectrometry data of muropeptides was acquired by the Mass Spectrometry Unit (UniMS), ITQB/iBET, Oeiras, Portugal.
id RCAP_9d8405ccfb96063d187133e0631f9808
oai_identifier_str oai:run.unl.pt:10362/76533
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrityGeneralThis study was funded by the European Research Council through grant ERC-2017-CoG 771709 (to M.G.P.), by Project LISBOA-01-0145-FEDER-007660 Microbiologia Molecular, Estrutural e Celular (to ITQB-NOVA), by the German Research Foundation (DFG; SCHN1284/1-2) to T.S. and FCT fellowship SFRH/BD/71993/2010 (J.M.M.). Mass spectrometry data of muropeptides was acquired by the Mass Spectrometry Unit (UniMS), ITQB/iBET, Oeiras, Portugal.Bacterial cells are surrounded by cell wall, whose main component is peptidoglycan (PG), a macromolecule that withstands the internal turgor of the cell. PG composition can vary considerably between species. The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family. FemX adds the first glycine of the cross bridge, while FemA and FemB add the second and the third, and the fourth and the fifth glycines, respectively. Of these, FemX was reported to be essential. To investigate the essentiality of FemAB, we constructed a conditional S. aureus mutant of the femAB operon. Depletion of femAB was lethal, with cells appearing as pseudomulticellular forms that eventually lyse due to extensive membrane rupture. This deleterious effect was mitigated by drastically increasing the osmolarity of the medium, indicating that pentaglycine crosslinks are required for S. aureus cells to withstand internal turgor. Despite the absence of canonical membrane targeting domains, FemA has been shown to localise at the membrane. To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding. Mutations in the α6 helix led to a sharp decrease in protein activity in vivo and in vitro but did not impair correct membrane localisation, indicating that FemA activity is not required for localisation. Our data indicates that, contrarily to what was previously thought, S. aureus cells do not survive in the absence of a pentaglycine cross bridge.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitMolecular, Structural and Cellular Microbiology (MOSTMICRO)RUNMonteiro, João M.Covas, GonçaloRausch, DanielaFilipe, Sérgio R.Schneider, TanjaSahl, Hans GeorgPinho, Mariana G.2019-07-25T22:33:43Z2019-12-012019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1038/s41598-019-41461-1eng2045-2322PURE: 12644811http://www.scopus.com/inward/record.url?scp=85063326118&partnerID=8YFLogxKhttps://doi.org/10.1038/s41598-019-41461-1info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:34:51Zoai:run.unl.pt:10362/76533Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:38.427635Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity
title The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity
spellingShingle The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity
Monteiro, João M.
General
title_short The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity
title_full The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity
title_fullStr The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity
title_full_unstemmed The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity
title_sort The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity
author Monteiro, João M.
author_facet Monteiro, João M.
Covas, Gonçalo
Rausch, Daniela
Filipe, Sérgio R.
Schneider, Tanja
Sahl, Hans Georg
Pinho, Mariana G.
author_role author
author2 Covas, Gonçalo
Rausch, Daniela
Filipe, Sérgio R.
Schneider, Tanja
Sahl, Hans Georg
Pinho, Mariana G.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
DCV - Departamento de Ciências da Vida
UCIBIO - Applied Molecular Biosciences Unit
Molecular, Structural and Cellular Microbiology (MOSTMICRO)
RUN
dc.contributor.author.fl_str_mv Monteiro, João M.
Covas, Gonçalo
Rausch, Daniela
Filipe, Sérgio R.
Schneider, Tanja
Sahl, Hans Georg
Pinho, Mariana G.
dc.subject.por.fl_str_mv General
topic General
description This study was funded by the European Research Council through grant ERC-2017-CoG 771709 (to M.G.P.), by Project LISBOA-01-0145-FEDER-007660 Microbiologia Molecular, Estrutural e Celular (to ITQB-NOVA), by the German Research Foundation (DFG; SCHN1284/1-2) to T.S. and FCT fellowship SFRH/BD/71993/2010 (J.M.M.). Mass spectrometry data of muropeptides was acquired by the Mass Spectrometry Unit (UniMS), ITQB/iBET, Oeiras, Portugal.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-25T22:33:43Z
2019-12-01
2019-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1038/s41598-019-41461-1
url https://doi.org/10.1038/s41598-019-41461-1
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 12644811
http://www.scopus.com/inward/record.url?scp=85063326118&partnerID=8YFLogxK
https://doi.org/10.1038/s41598-019-41461-1
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137976941281280

Registros relacionados