Occurrence of Plant-Uncoupling Mitochondrial Protein (PUMP) in Diverse Organs and Tissues of Several Plants
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/7606 https://doi.org/10.1023/A:1005648226431 |
Resumo: | The presence of plant-uncoupling mitochondrial protein (PUMP), previously described by Vercesi et al. (1995), was screened in mitochondria of various organs or tissues of several plant species. This was done functionally, by monitoring purine nucleotide-sensitive linoleic acid-induced uncoupling, or by Western blots. The following findings were established: (1) PUMP was found in most of the higher plants tested; (2) since ATP inhibition of linoleic acid-induced membrane potential decrease varied, PUMP content might differ in different plant tissues, as observed with mitochondria from maize roots, maize seeds, spinach leaves, wheat shoots, carrot roots, cauliflower, broccoli, maize shoots, turnip root, and potato calli. Western blots also indicated PUMP presence in oat shoots, carnation petals, onion bulbs, red beet root, green cabbage, and Sedum leaves. (3) PUMP was not detected in mushrooms. We conclude that PUMP is likely present in the mitochondria of organs and tissues of all higher plants. |
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Occurrence of Plant-Uncoupling Mitochondrial Protein (PUMP) in Diverse Organs and Tissues of Several PlantsThe presence of plant-uncoupling mitochondrial protein (PUMP), previously described by Vercesi et al. (1995), was screened in mitochondria of various organs or tissues of several plant species. This was done functionally, by monitoring purine nucleotide-sensitive linoleic acid-induced uncoupling, or by Western blots. The following findings were established: (1) PUMP was found in most of the higher plants tested; (2) since ATP inhibition of linoleic acid-induced membrane potential decrease varied, PUMP content might differ in different plant tissues, as observed with mitochondria from maize roots, maize seeds, spinach leaves, wheat shoots, carrot roots, cauliflower, broccoli, maize shoots, turnip root, and potato calli. Western blots also indicated PUMP presence in oat shoots, carnation petals, onion bulbs, red beet root, green cabbage, and Sedum leaves. (3) PUMP was not detected in mushrooms. We conclude that PUMP is likely present in the mitochondria of organs and tissues of all higher plants.2000info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/7606http://hdl.handle.net/10316/7606https://doi.org/10.1023/A:1005648226431engJournal of Bioenergetics and Biomembranes. 32:6 (2000) 549-561Ježek, PetrŽácková, MarkétaKošarová, JanaRodrigues, Elsa T. S.Madeira, Vítor M. C.Vicente, Joaquim A. F.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2019-05-28T14:45:10Zoai:estudogeral.uc.pt:10316/7606Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:52.506053Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Occurrence of Plant-Uncoupling Mitochondrial Protein (PUMP) in Diverse Organs and Tissues of Several Plants |
title |
Occurrence of Plant-Uncoupling Mitochondrial Protein (PUMP) in Diverse Organs and Tissues of Several Plants |
spellingShingle |
Occurrence of Plant-Uncoupling Mitochondrial Protein (PUMP) in Diverse Organs and Tissues of Several Plants Ježek, Petr |
title_short |
Occurrence of Plant-Uncoupling Mitochondrial Protein (PUMP) in Diverse Organs and Tissues of Several Plants |
title_full |
Occurrence of Plant-Uncoupling Mitochondrial Protein (PUMP) in Diverse Organs and Tissues of Several Plants |
title_fullStr |
Occurrence of Plant-Uncoupling Mitochondrial Protein (PUMP) in Diverse Organs and Tissues of Several Plants |
title_full_unstemmed |
Occurrence of Plant-Uncoupling Mitochondrial Protein (PUMP) in Diverse Organs and Tissues of Several Plants |
title_sort |
Occurrence of Plant-Uncoupling Mitochondrial Protein (PUMP) in Diverse Organs and Tissues of Several Plants |
author |
Ježek, Petr |
author_facet |
Ježek, Petr Žácková, Markéta Košarová, Jana Rodrigues, Elsa T. S. Madeira, Vítor M. C. Vicente, Joaquim A. F. |
author_role |
author |
author2 |
Žácková, Markéta Košarová, Jana Rodrigues, Elsa T. S. Madeira, Vítor M. C. Vicente, Joaquim A. F. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ježek, Petr Žácková, Markéta Košarová, Jana Rodrigues, Elsa T. S. Madeira, Vítor M. C. Vicente, Joaquim A. F. |
description |
The presence of plant-uncoupling mitochondrial protein (PUMP), previously described by Vercesi et al. (1995), was screened in mitochondria of various organs or tissues of several plant species. This was done functionally, by monitoring purine nucleotide-sensitive linoleic acid-induced uncoupling, or by Western blots. The following findings were established: (1) PUMP was found in most of the higher plants tested; (2) since ATP inhibition of linoleic acid-induced membrane potential decrease varied, PUMP content might differ in different plant tissues, as observed with mitochondria from maize roots, maize seeds, spinach leaves, wheat shoots, carrot roots, cauliflower, broccoli, maize shoots, turnip root, and potato calli. Western blots also indicated PUMP presence in oat shoots, carnation petals, onion bulbs, red beet root, green cabbage, and Sedum leaves. (3) PUMP was not detected in mushrooms. We conclude that PUMP is likely present in the mitochondria of organs and tissues of all higher plants. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/7606 http://hdl.handle.net/10316/7606 https://doi.org/10.1023/A:1005648226431 |
url |
http://hdl.handle.net/10316/7606 https://doi.org/10.1023/A:1005648226431 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Bioenergetics and Biomembranes. 32:6 (2000) 549-561 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133844857683968 |