Microbial metabolic potential of phenol degradation in wastewater treatment plant of crude oil refinery: analysis of metagenomes and characterization of isolates
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/65506 |
Resumo: | The drilling, processing and transportation of oil are the main sources of pollution in water and soil. The current work analyzes the microbial diversity and aromatic compounds degradation potential in the metagenomes of communities in the wastewater treatment plant (WWTP) of a crude oil refinery. By focusing on the degradation of phenol, we observed the involvement of diverse indigenous microbial communities at different steps of the WWTP. The anaerobic bacterial and archaeal genera were replaced by aerobic and facultative anaerobic bacteria through the biological treatment processes. The phyla Proteobacteria, Bacteroidetes and Planctomycetes were dominating at different stages of the treatment. Most of the established protein sequences of the phenol degradation key enzymes belonged to bacteria from the class Alphaproteobacteria. From 35 isolated strains, 14 were able to grow on aromatic compounds, whereas several phenolic compound-degrading strains also degraded aliphatic hydrocarbons. Two strains, <i>Acinetobacter venetianus</i> ICP1 and <i>Pseudomonas oleovorans</i> ICTN13, were able to degrade various aromatic and aliphatic pollutants and were further characterized by whole genome sequencing and cultivation experiments in the presence of phenol to ascertain their metabolic capacity in phenol degradation. When grown alone, the intermediates of catechol degradation, the meta or ortho pathways, accumulated into the growth environment of these strains. In the mixed cultures of the strains ICP1 and ICTN13, phenol was degraded via cooperation, in which the strain ICP1 was responsible for the adherence of cells and ICTN13 diminished the accumulation of toxic intermediates. |
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Microbial metabolic potential of phenol degradation in wastewater treatment plant of crude oil refinery: analysis of metagenomes and characterization of isolatesphenolbacterial communityoil refinery wastewatermetagenomePseudomonasAcinetobacterScience & TechnologyThe drilling, processing and transportation of oil are the main sources of pollution in water and soil. The current work analyzes the microbial diversity and aromatic compounds degradation potential in the metagenomes of communities in the wastewater treatment plant (WWTP) of a crude oil refinery. By focusing on the degradation of phenol, we observed the involvement of diverse indigenous microbial communities at different steps of the WWTP. The anaerobic bacterial and archaeal genera were replaced by aerobic and facultative anaerobic bacteria through the biological treatment processes. The phyla Proteobacteria, Bacteroidetes and Planctomycetes were dominating at different stages of the treatment. Most of the established protein sequences of the phenol degradation key enzymes belonged to bacteria from the class Alphaproteobacteria. From 35 isolated strains, 14 were able to grow on aromatic compounds, whereas several phenolic compound-degrading strains also degraded aliphatic hydrocarbons. Two strains, <i>Acinetobacter venetianus</i> ICP1 and <i>Pseudomonas oleovorans</i> ICTN13, were able to degrade various aromatic and aliphatic pollutants and were further characterized by whole genome sequencing and cultivation experiments in the presence of phenol to ascertain their metabolic capacity in phenol degradation. When grown alone, the intermediates of catechol degradation, the meta or ortho pathways, accumulated into the growth environment of these strains. In the mixed cultures of the strains ICP1 and ICTN13, phenol was degraded via cooperation, in which the strain ICP1 was responsible for the adherence of cells and ICTN13 diminished the accumulation of toxic intermediates.This study was supported by the ERA-NET project WRANA (Inno-INDIGO/0004/2014), and by the Institutional Research Funding IUT20-19 from Eesti Teadusagentuur (Estonian Research Council) to MK, by the strategic program UID/BIA/04050/2013 (POCI-01-0145-FEDER-007569) funded by national funds through the FCT I.P. and by the ERDF through the COMPETE2020-Programa Operacional Competitividade e Internacionalização (POCI) to PMS, and by Department of Biotechnology, Ministry of Science and Technology, New Delhi, BT/IN/INNO-INDIGO/29/AK/2015-16 to AK. The isolated strains are deposited in the Collection of Environmental and Laboratory Microbial Strains (CELMS; financed by the Estonian Ministry of Education and Research) (RLOMRCELMS) the public catalogue of which is available on the Estonian Electronic Microbial dataBase (EEMB) website http://eemb.ut.ee.Multidisciplinary Digital Publishing InstituteUniversidade do MinhoViggor, SigneJõesaar, MerikeSoares-Castro, PedroIlmjärv, TanelSantos, P. M.Kapley, AtyaKivisaar, Maia2020-04-302020-04-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65506engViggor, S.; Jõesaar, M.; Soares-Castro, P.; Ilmjärv, T.; Santos, P.M.; Kapley, A.; Kivisaar, M. Microbial Metabolic Potential of Phenol Degradation in Wastewater Treatment Plant of Crude Oil Refinery: Analysis of Metagenomes and Characterization of Isolates. Microorganisms 2020, 8, 652.10.3390/microorganisms8050652https://www.mdpi.com/2076-2607/8/5/652info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:36:45Zoai:repositorium.sdum.uminho.pt:1822/65506Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:32:55.459042Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Microbial metabolic potential of phenol degradation in wastewater treatment plant of crude oil refinery: analysis of metagenomes and characterization of isolates |
title |
Microbial metabolic potential of phenol degradation in wastewater treatment plant of crude oil refinery: analysis of metagenomes and characterization of isolates |
spellingShingle |
Microbial metabolic potential of phenol degradation in wastewater treatment plant of crude oil refinery: analysis of metagenomes and characterization of isolates Viggor, Signe phenol bacterial community oil refinery wastewater metagenome Pseudomonas Acinetobacter Science & Technology |
title_short |
Microbial metabolic potential of phenol degradation in wastewater treatment plant of crude oil refinery: analysis of metagenomes and characterization of isolates |
title_full |
Microbial metabolic potential of phenol degradation in wastewater treatment plant of crude oil refinery: analysis of metagenomes and characterization of isolates |
title_fullStr |
Microbial metabolic potential of phenol degradation in wastewater treatment plant of crude oil refinery: analysis of metagenomes and characterization of isolates |
title_full_unstemmed |
Microbial metabolic potential of phenol degradation in wastewater treatment plant of crude oil refinery: analysis of metagenomes and characterization of isolates |
title_sort |
Microbial metabolic potential of phenol degradation in wastewater treatment plant of crude oil refinery: analysis of metagenomes and characterization of isolates |
author |
Viggor, Signe |
author_facet |
Viggor, Signe Jõesaar, Merike Soares-Castro, Pedro Ilmjärv, Tanel Santos, P. M. Kapley, Atya Kivisaar, Maia |
author_role |
author |
author2 |
Jõesaar, Merike Soares-Castro, Pedro Ilmjärv, Tanel Santos, P. M. Kapley, Atya Kivisaar, Maia |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Viggor, Signe Jõesaar, Merike Soares-Castro, Pedro Ilmjärv, Tanel Santos, P. M. Kapley, Atya Kivisaar, Maia |
dc.subject.por.fl_str_mv |
phenol bacterial community oil refinery wastewater metagenome Pseudomonas Acinetobacter Science & Technology |
topic |
phenol bacterial community oil refinery wastewater metagenome Pseudomonas Acinetobacter Science & Technology |
description |
The drilling, processing and transportation of oil are the main sources of pollution in water and soil. The current work analyzes the microbial diversity and aromatic compounds degradation potential in the metagenomes of communities in the wastewater treatment plant (WWTP) of a crude oil refinery. By focusing on the degradation of phenol, we observed the involvement of diverse indigenous microbial communities at different steps of the WWTP. The anaerobic bacterial and archaeal genera were replaced by aerobic and facultative anaerobic bacteria through the biological treatment processes. The phyla Proteobacteria, Bacteroidetes and Planctomycetes were dominating at different stages of the treatment. Most of the established protein sequences of the phenol degradation key enzymes belonged to bacteria from the class Alphaproteobacteria. From 35 isolated strains, 14 were able to grow on aromatic compounds, whereas several phenolic compound-degrading strains also degraded aliphatic hydrocarbons. Two strains, <i>Acinetobacter venetianus</i> ICP1 and <i>Pseudomonas oleovorans</i> ICTN13, were able to degrade various aromatic and aliphatic pollutants and were further characterized by whole genome sequencing and cultivation experiments in the presence of phenol to ascertain their metabolic capacity in phenol degradation. When grown alone, the intermediates of catechol degradation, the meta or ortho pathways, accumulated into the growth environment of these strains. In the mixed cultures of the strains ICP1 and ICTN13, phenol was degraded via cooperation, in which the strain ICP1 was responsible for the adherence of cells and ICTN13 diminished the accumulation of toxic intermediates. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-04-30 2020-04-30T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/65506 |
url |
http://hdl.handle.net/1822/65506 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Viggor, S.; Jõesaar, M.; Soares-Castro, P.; Ilmjärv, T.; Santos, P.M.; Kapley, A.; Kivisaar, M. Microbial Metabolic Potential of Phenol Degradation in Wastewater Treatment Plant of Crude Oil Refinery: Analysis of Metagenomes and Characterization of Isolates. Microorganisms 2020, 8, 652. 10.3390/microorganisms8050652 https://www.mdpi.com/2076-2607/8/5/652 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132843349114880 |