Revolutionizing disease modeling: the emergence of organoids in cellular systems
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/83599 |
Resumo: | Cellular models have created opportunities to explore the characteristics of human diseases through well-established protocols, while avoiding the ethical restrictions associated with post-mortem studies and the costs associated with researching animal models. The capability of cell reprogramming, such as induced pluripotent stem cells (iPSCs) technology, solved the complications associated with human embryonic stem cells (hESC) usage. Moreover, iPSCs made significant contributions for human medicine, such as in diagnosis, therapeutic and regenerative medicine. The two-dimensional (2D) models allowed for monolayer cellular culture in vitro; however, they were surpassed by the three-dimensional (3D) cell culture system. The 3D cell culture provides higher cell–cell contact and a multi-layered cell culture, which more closely respects cellular morphology and polarity. It is more tightly able to resemble conditions in vivo and a closer approach to the architecture of human tissues, such as human organoids. Organoids are 3D cellular structures that mimic the architecture and function of native tissues. They are generated in vitro from stem cells or differentiated cells, such as epithelial or neural cells, and are used to study organ development, disease modeling, and drug discovery. Organoids have become a powerful tool for understanding the cellular and molecular mechanisms underlying human physiology, providing new insights into the pathogenesis of cancer, metabolic diseases, and brain disorders. Although organoid technology is up-and-coming, it also has some limitations that require improvements. |
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Revolutionizing disease modeling: the emergence of organoids in cellular systemsCell cultureiPSCsDisease modelingOrganoidsOrganoids applicationsOrganoids limitationsScience & TechnologyCellular models have created opportunities to explore the characteristics of human diseases through well-established protocols, while avoiding the ethical restrictions associated with post-mortem studies and the costs associated with researching animal models. The capability of cell reprogramming, such as induced pluripotent stem cells (iPSCs) technology, solved the complications associated with human embryonic stem cells (hESC) usage. Moreover, iPSCs made significant contributions for human medicine, such as in diagnosis, therapeutic and regenerative medicine. The two-dimensional (2D) models allowed for monolayer cellular culture in vitro; however, they were surpassed by the three-dimensional (3D) cell culture system. The 3D cell culture provides higher cell–cell contact and a multi-layered cell culture, which more closely respects cellular morphology and polarity. It is more tightly able to resemble conditions in vivo and a closer approach to the architecture of human tissues, such as human organoids. Organoids are 3D cellular structures that mimic the architecture and function of native tissues. They are generated in vitro from stem cells or differentiated cells, such as epithelial or neural cells, and are used to study organ development, disease modeling, and drug discovery. Organoids have become a powerful tool for understanding the cellular and molecular mechanisms underlying human physiology, providing new insights into the pathogenesis of cancer, metabolic diseases, and brain disorders. Although organoid technology is up-and-coming, it also has some limitations that require improvements.This work was funded by national funds, through the Foundation for Science and Technology (FCT), a Ph.D. grant SFRH/BD/131540/2017, COVID/BD/152416/2022, UMINHO/BIMCNCG/2022/143, and project PTDC/SAU-PAR/2766/2021.info:eu-repo/semantics/publishedVersionMultidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoPedrosa, Ana Rita SilvaSalgado, A. J.Ferreira, Pedro Eduardo Mendes2023-03-1810000-01-01T00:00:00Z2023-03-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/83599engPedrosa, Ana; Salgado, António José; Ferreira, Pedro Eduardo, Revolutionizing disease modeling: the emergence of organoids in cellular systems. Cells, 12(6), 930, 20232073-440910.3390/cells1206093036980271930https://www.mdpi.com/journal/cellsinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:12:43Zoai:repositorium.sdum.uminho.pt:1822/83599Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:04:39.531381Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Revolutionizing disease modeling: the emergence of organoids in cellular systems |
title |
Revolutionizing disease modeling: the emergence of organoids in cellular systems |
spellingShingle |
Revolutionizing disease modeling: the emergence of organoids in cellular systems Pedrosa, Ana Rita Silva Cell culture iPSCs Disease modeling Organoids Organoids applications Organoids limitations Science & Technology |
title_short |
Revolutionizing disease modeling: the emergence of organoids in cellular systems |
title_full |
Revolutionizing disease modeling: the emergence of organoids in cellular systems |
title_fullStr |
Revolutionizing disease modeling: the emergence of organoids in cellular systems |
title_full_unstemmed |
Revolutionizing disease modeling: the emergence of organoids in cellular systems |
title_sort |
Revolutionizing disease modeling: the emergence of organoids in cellular systems |
author |
Pedrosa, Ana Rita Silva |
author_facet |
Pedrosa, Ana Rita Silva Salgado, A. J. Ferreira, Pedro Eduardo Mendes |
author_role |
author |
author2 |
Salgado, A. J. Ferreira, Pedro Eduardo Mendes |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pedrosa, Ana Rita Silva Salgado, A. J. Ferreira, Pedro Eduardo Mendes |
dc.subject.por.fl_str_mv |
Cell culture iPSCs Disease modeling Organoids Organoids applications Organoids limitations Science & Technology |
topic |
Cell culture iPSCs Disease modeling Organoids Organoids applications Organoids limitations Science & Technology |
description |
Cellular models have created opportunities to explore the characteristics of human diseases through well-established protocols, while avoiding the ethical restrictions associated with post-mortem studies and the costs associated with researching animal models. The capability of cell reprogramming, such as induced pluripotent stem cells (iPSCs) technology, solved the complications associated with human embryonic stem cells (hESC) usage. Moreover, iPSCs made significant contributions for human medicine, such as in diagnosis, therapeutic and regenerative medicine. The two-dimensional (2D) models allowed for monolayer cellular culture in vitro; however, they were surpassed by the three-dimensional (3D) cell culture system. The 3D cell culture provides higher cell–cell contact and a multi-layered cell culture, which more closely respects cellular morphology and polarity. It is more tightly able to resemble conditions in vivo and a closer approach to the architecture of human tissues, such as human organoids. Organoids are 3D cellular structures that mimic the architecture and function of native tissues. They are generated in vitro from stem cells or differentiated cells, such as epithelial or neural cells, and are used to study organ development, disease modeling, and drug discovery. Organoids have become a powerful tool for understanding the cellular and molecular mechanisms underlying human physiology, providing new insights into the pathogenesis of cancer, metabolic diseases, and brain disorders. Although organoid technology is up-and-coming, it also has some limitations that require improvements. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
10000-01-01T00:00:00Z 2023-03-18 2023-03-18T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/83599 |
url |
https://hdl.handle.net/1822/83599 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pedrosa, Ana; Salgado, António José; Ferreira, Pedro Eduardo, Revolutionizing disease modeling: the emergence of organoids in cellular systems. Cells, 12(6), 930, 2023 2073-4409 10.3390/cells12060930 36980271 930 https://www.mdpi.com/journal/cells |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132457071542272 |