Femtosecond laser microstructured Alumina toughened Zirconia: a new strategy to improve osteogenic differentiation of hMSCs
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/8176 |
Resumo: | The use of topographic patterns has been a continuously growing area of research for tissue engineering and it is widely accepted that the surface topography of biomaterials can influence and modulate the initial biological response. Ultrafast lasers are extremely powerful tools to machine and pattern the surface of a wide range of biomaterials, however, only few work has been performed on ceramics with the intent of biomedical applications, and the biological characterization of these structured materials is scarce. In this work, relevance is given to the biological performance of such materials. A femtosecond laser ablation technique was used to modify Alumina toughened Zirconia (ATZ) surface topography, developing surfaces structured at the micro and nanoscale levels (mu ATZ), in a controlled and reproducible manner. Materials characterization was performed before and after laser treatment, and both materials were compared in terms of osteogenic response of human bone marrow derived mesenchymal stem cells cultured under basal conditions, expecting that the micro/nanofeatures will improve the biological response of cells. Cells metabolic activity and proliferation increased with the culture time and surface microtopography modulated cells alignment and guided proliferation. The modified surface, displayed significantly higher expression of osteogenic transcription factors and genes and, additionally, the formation of a mineralized extracellular matrix, when compared to the control surface, i.e. unmodified ATZ. |
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Femtosecond laser microstructured Alumina toughened Zirconia: a new strategy to improve osteogenic differentiation of hMSCsMicrostructured biomaterialsFemtosecond laserSurface topographyOsteogenic differentiationThe use of topographic patterns has been a continuously growing area of research for tissue engineering and it is widely accepted that the surface topography of biomaterials can influence and modulate the initial biological response. Ultrafast lasers are extremely powerful tools to machine and pattern the surface of a wide range of biomaterials, however, only few work has been performed on ceramics with the intent of biomedical applications, and the biological characterization of these structured materials is scarce. In this work, relevance is given to the biological performance of such materials. A femtosecond laser ablation technique was used to modify Alumina toughened Zirconia (ATZ) surface topography, developing surfaces structured at the micro and nanoscale levels (mu ATZ), in a controlled and reproducible manner. Materials characterization was performed before and after laser treatment, and both materials were compared in terms of osteogenic response of human bone marrow derived mesenchymal stem cells cultured under basal conditions, expecting that the micro/nanofeatures will improve the biological response of cells. Cells metabolic activity and proliferation increased with the culture time and surface microtopography modulated cells alignment and guided proliferation. The modified surface, displayed significantly higher expression of osteogenic transcription factors and genes and, additionally, the formation of a mineralized extracellular matrix, when compared to the control surface, i.e. unmodified ATZ.ElsevierRCIPLCarvalho, AngelaCangueiro, LilianaOliveira, VitorVilar, RuiFernandes, Maria HelenaMonteiro, Fernando2018-03-01T13:54:48Z2018-03-302018-03-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/8176engCARVALHO, Ângela; [et al] – Femtosecond laser microstructured Alumina toughened Zirconia: A new strategy to improve osteogenic differentiation of hMSCs. Applied Surface Science. ISSN 0169-4332. Vol. 435 (2018), pp. 1237-12450169-433210.1016/j.apsusc.2017.11.206metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T09:55:13Zoai:repositorio.ipl.pt:10400.21/8176Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:16:59.126350Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Femtosecond laser microstructured Alumina toughened Zirconia: a new strategy to improve osteogenic differentiation of hMSCs |
title |
Femtosecond laser microstructured Alumina toughened Zirconia: a new strategy to improve osteogenic differentiation of hMSCs |
spellingShingle |
Femtosecond laser microstructured Alumina toughened Zirconia: a new strategy to improve osteogenic differentiation of hMSCs Carvalho, Angela Microstructured biomaterials Femtosecond laser Surface topography Osteogenic differentiation |
title_short |
Femtosecond laser microstructured Alumina toughened Zirconia: a new strategy to improve osteogenic differentiation of hMSCs |
title_full |
Femtosecond laser microstructured Alumina toughened Zirconia: a new strategy to improve osteogenic differentiation of hMSCs |
title_fullStr |
Femtosecond laser microstructured Alumina toughened Zirconia: a new strategy to improve osteogenic differentiation of hMSCs |
title_full_unstemmed |
Femtosecond laser microstructured Alumina toughened Zirconia: a new strategy to improve osteogenic differentiation of hMSCs |
title_sort |
Femtosecond laser microstructured Alumina toughened Zirconia: a new strategy to improve osteogenic differentiation of hMSCs |
author |
Carvalho, Angela |
author_facet |
Carvalho, Angela Cangueiro, Liliana Oliveira, Vitor Vilar, Rui Fernandes, Maria Helena Monteiro, Fernando |
author_role |
author |
author2 |
Cangueiro, Liliana Oliveira, Vitor Vilar, Rui Fernandes, Maria Helena Monteiro, Fernando |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Carvalho, Angela Cangueiro, Liliana Oliveira, Vitor Vilar, Rui Fernandes, Maria Helena Monteiro, Fernando |
dc.subject.por.fl_str_mv |
Microstructured biomaterials Femtosecond laser Surface topography Osteogenic differentiation |
topic |
Microstructured biomaterials Femtosecond laser Surface topography Osteogenic differentiation |
description |
The use of topographic patterns has been a continuously growing area of research for tissue engineering and it is widely accepted that the surface topography of biomaterials can influence and modulate the initial biological response. Ultrafast lasers are extremely powerful tools to machine and pattern the surface of a wide range of biomaterials, however, only few work has been performed on ceramics with the intent of biomedical applications, and the biological characterization of these structured materials is scarce. In this work, relevance is given to the biological performance of such materials. A femtosecond laser ablation technique was used to modify Alumina toughened Zirconia (ATZ) surface topography, developing surfaces structured at the micro and nanoscale levels (mu ATZ), in a controlled and reproducible manner. Materials characterization was performed before and after laser treatment, and both materials were compared in terms of osteogenic response of human bone marrow derived mesenchymal stem cells cultured under basal conditions, expecting that the micro/nanofeatures will improve the biological response of cells. Cells metabolic activity and proliferation increased with the culture time and surface microtopography modulated cells alignment and guided proliferation. The modified surface, displayed significantly higher expression of osteogenic transcription factors and genes and, additionally, the formation of a mineralized extracellular matrix, when compared to the control surface, i.e. unmodified ATZ. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-01T13:54:48Z 2018-03-30 2018-03-30T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/8176 |
url |
http://hdl.handle.net/10400.21/8176 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
CARVALHO, Ângela; [et al] – Femtosecond laser microstructured Alumina toughened Zirconia: A new strategy to improve osteogenic differentiation of hMSCs. Applied Surface Science. ISSN 0169-4332. Vol. 435 (2018), pp. 1237-1245 0169-4332 10.1016/j.apsusc.2017.11.206 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133431552016384 |