Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/6755 |
Resumo: | The effects of dilution rate and substrate feed concentration on continuous glycerol fermentation by Clostridium butyricum VPI 3266, a natural 1,3-propanediol producer, were evaluated in this work. A high and constant 1,3-propanediol yield (around 0.65 mol/mol), close to the theoretical value, was obtained irrespective of substrate feed concentration or dilution rate. Improvement of 1,3-propanediol volumetric productivity was achieved by increasing the dilution rate, at a fixed feed substrate concentration of 30, 60 or 70 g l-1. Higher 1,3-propanediol final concentrations and volumetric productivities were also obtained when glycerol feed concentration was increased from 30 to 60 g l-1, at D=0.05–0.3 h-1, and from 60–70 g l-1, at D=0.05 and 0.1 h-1. 30 g l-1 of 1,3-propanediol and the highest reported value of productivity, 10.3 g l-1 h-1, was achieved at D=0.30 h-1 and 60 g l-1 of feed glycerol. A switch to an acetate/butyrate ratio higher than one was observed for 60 g l-1 of feed glycerol and a dilution rate higher than 0.10 h-1; moreover, at D=0.30 h-1 3-hydroxypropionaldehyde accumulation was observed for the first time in the fermentation broth of C. butyricum. |
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Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivityThe effects of dilution rate and substrate feed concentration on continuous glycerol fermentation by Clostridium butyricum VPI 3266, a natural 1,3-propanediol producer, were evaluated in this work. A high and constant 1,3-propanediol yield (around 0.65 mol/mol), close to the theoretical value, was obtained irrespective of substrate feed concentration or dilution rate. Improvement of 1,3-propanediol volumetric productivity was achieved by increasing the dilution rate, at a fixed feed substrate concentration of 30, 60 or 70 g l-1. Higher 1,3-propanediol final concentrations and volumetric productivities were also obtained when glycerol feed concentration was increased from 30 to 60 g l-1, at D=0.05–0.3 h-1, and from 60–70 g l-1, at D=0.05 and 0.1 h-1. 30 g l-1 of 1,3-propanediol and the highest reported value of productivity, 10.3 g l-1 h-1, was achieved at D=0.30 h-1 and 60 g l-1 of feed glycerol. A switch to an acetate/butyrate ratio higher than one was observed for 60 g l-1 of feed glycerol and a dilution rate higher than 0.10 h-1; moreover, at D=0.30 h-1 3-hydroxypropionaldehyde accumulation was observed for the first time in the fermentation broth of C. butyricum.SpringerVeritati - Repositório Institucional da Universidade Católica PortuguesaGonzález-Pajuelo, M.Andrade, J. C.Vasconcelos, I.2011-10-22T08:58:59Z2005-072005-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/6755eng"Journal of Industrial Microbiology & Biotechnology" . ISSN 1367-5435. 32:9 (2005) 391-396info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T11:59:07Zoai:repositorio.ucp.pt:10400.14/6755Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T11:59:07Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity |
title |
Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity |
spellingShingle |
Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity González-Pajuelo, M. |
title_short |
Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity |
title_full |
Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity |
title_fullStr |
Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity |
title_full_unstemmed |
Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity |
title_sort |
Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity |
author |
González-Pajuelo, M. |
author_facet |
González-Pajuelo, M. Andrade, J. C. Vasconcelos, I. |
author_role |
author |
author2 |
Andrade, J. C. Vasconcelos, I. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
González-Pajuelo, M. Andrade, J. C. Vasconcelos, I. |
description |
The effects of dilution rate and substrate feed concentration on continuous glycerol fermentation by Clostridium butyricum VPI 3266, a natural 1,3-propanediol producer, were evaluated in this work. A high and constant 1,3-propanediol yield (around 0.65 mol/mol), close to the theoretical value, was obtained irrespective of substrate feed concentration or dilution rate. Improvement of 1,3-propanediol volumetric productivity was achieved by increasing the dilution rate, at a fixed feed substrate concentration of 30, 60 or 70 g l-1. Higher 1,3-propanediol final concentrations and volumetric productivities were also obtained when glycerol feed concentration was increased from 30 to 60 g l-1, at D=0.05–0.3 h-1, and from 60–70 g l-1, at D=0.05 and 0.1 h-1. 30 g l-1 of 1,3-propanediol and the highest reported value of productivity, 10.3 g l-1 h-1, was achieved at D=0.30 h-1 and 60 g l-1 of feed glycerol. A switch to an acetate/butyrate ratio higher than one was observed for 60 g l-1 of feed glycerol and a dilution rate higher than 0.10 h-1; moreover, at D=0.30 h-1 3-hydroxypropionaldehyde accumulation was observed for the first time in the fermentation broth of C. butyricum. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-07 2005-07-01T00:00:00Z 2011-10-22T08:58:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/6755 |
url |
http://hdl.handle.net/10400.14/6755 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Journal of Industrial Microbiology & Biotechnology" . ISSN 1367-5435. 32:9 (2005) 391-396 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817546697515991040 |