Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity

Detalhes bibliográficos
Autor(a) principal: González-Pajuelo, M.
Data de Publicação: 2005
Outros Autores: Andrade, J. C., Vasconcelos, I.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/6755
Resumo: The effects of dilution rate and substrate feed concentration on continuous glycerol fermentation by Clostridium butyricum VPI 3266, a natural 1,3-propanediol producer, were evaluated in this work. A high and constant 1,3-propanediol yield (around 0.65 mol/mol), close to the theoretical value, was obtained irrespective of substrate feed concentration or dilution rate. Improvement of 1,3-propanediol volumetric productivity was achieved by increasing the dilution rate, at a fixed feed substrate concentration of 30, 60 or 70 g l-1. Higher 1,3-propanediol final concentrations and volumetric productivities were also obtained when glycerol feed concentration was increased from 30 to 60 g l-1, at D=0.05–0.3 h-1, and from 60–70 g l-1, at D=0.05 and 0.1 h-1. 30 g l-1 of 1,3-propanediol and the highest reported value of productivity, 10.3 g l-1 h-1, was achieved at D=0.30 h-1 and 60 g l-1 of feed glycerol. A switch to an acetate/butyrate ratio higher than one was observed for 60 g l-1 of feed glycerol and a dilution rate higher than 0.10 h-1; moreover, at D=0.30 h-1 3-hydroxypropionaldehyde accumulation was observed for the first time in the fermentation broth of C. butyricum.
id RCAP_9e0d410c484c2ec5b940083ac3fbe9a1
oai_identifier_str oai:repositorio.ucp.pt:10400.14/6755
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivityThe effects of dilution rate and substrate feed concentration on continuous glycerol fermentation by Clostridium butyricum VPI 3266, a natural 1,3-propanediol producer, were evaluated in this work. A high and constant 1,3-propanediol yield (around 0.65 mol/mol), close to the theoretical value, was obtained irrespective of substrate feed concentration or dilution rate. Improvement of 1,3-propanediol volumetric productivity was achieved by increasing the dilution rate, at a fixed feed substrate concentration of 30, 60 or 70 g l-1. Higher 1,3-propanediol final concentrations and volumetric productivities were also obtained when glycerol feed concentration was increased from 30 to 60 g l-1, at D=0.05–0.3 h-1, and from 60–70 g l-1, at D=0.05 and 0.1 h-1. 30 g l-1 of 1,3-propanediol and the highest reported value of productivity, 10.3 g l-1 h-1, was achieved at D=0.30 h-1 and 60 g l-1 of feed glycerol. A switch to an acetate/butyrate ratio higher than one was observed for 60 g l-1 of feed glycerol and a dilution rate higher than 0.10 h-1; moreover, at D=0.30 h-1 3-hydroxypropionaldehyde accumulation was observed for the first time in the fermentation broth of C. butyricum.SpringerVeritati - Repositório Institucional da Universidade Católica PortuguesaGonzález-Pajuelo, M.Andrade, J. C.Vasconcelos, I.2011-10-22T08:58:59Z2005-072005-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/6755eng"Journal of Industrial Microbiology & Biotechnology" . ISSN 1367-5435. 32:9 (2005) 391-396info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-06T11:59:07Zoai:repositorio.ucp.pt:10400.14/6755Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-06T11:59:07Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity
title Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity
spellingShingle Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity
González-Pajuelo, M.
title_short Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity
title_full Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity
title_fullStr Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity
title_full_unstemmed Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity
title_sort Production of 1,3-Propanediol by Clostridium butyricum VPI 3266 in continuous cultures with high yield and productivity
author González-Pajuelo, M.
author_facet González-Pajuelo, M.
Andrade, J. C.
Vasconcelos, I.
author_role author
author2 Andrade, J. C.
Vasconcelos, I.
author2_role author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv González-Pajuelo, M.
Andrade, J. C.
Vasconcelos, I.
description The effects of dilution rate and substrate feed concentration on continuous glycerol fermentation by Clostridium butyricum VPI 3266, a natural 1,3-propanediol producer, were evaluated in this work. A high and constant 1,3-propanediol yield (around 0.65 mol/mol), close to the theoretical value, was obtained irrespective of substrate feed concentration or dilution rate. Improvement of 1,3-propanediol volumetric productivity was achieved by increasing the dilution rate, at a fixed feed substrate concentration of 30, 60 or 70 g l-1. Higher 1,3-propanediol final concentrations and volumetric productivities were also obtained when glycerol feed concentration was increased from 30 to 60 g l-1, at D=0.05–0.3 h-1, and from 60–70 g l-1, at D=0.05 and 0.1 h-1. 30 g l-1 of 1,3-propanediol and the highest reported value of productivity, 10.3 g l-1 h-1, was achieved at D=0.30 h-1 and 60 g l-1 of feed glycerol. A switch to an acetate/butyrate ratio higher than one was observed for 60 g l-1 of feed glycerol and a dilution rate higher than 0.10 h-1; moreover, at D=0.30 h-1 3-hydroxypropionaldehyde accumulation was observed for the first time in the fermentation broth of C. butyricum.
publishDate 2005
dc.date.none.fl_str_mv 2005-07
2005-07-01T00:00:00Z
2011-10-22T08:58:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/6755
url http://hdl.handle.net/10400.14/6755
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of Industrial Microbiology & Biotechnology" . ISSN 1367-5435. 32:9 (2005) 391-396
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817546697515991040