The role of secreted aspartyl proteinases in Candida tropicalis invasion and damage of oral mucosa

Detalhes bibliográficos
Autor(a) principal: Silva, Sónia Carina
Data de Publicação: 2011
Outros Autores: Hooper, S. J., Henriques, Mariana, Oliveira, Rosário, Azeredo, Joana, Williams, D. W.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/22710
Resumo: Candida virulence attributes include the ability to colonize and invade host tissues, and the secretion of hydrolytic enzymes. Although Candida albicans is regarded as the principal fungi causing infections in humans, other species, particularly Candida tropicalis, are increasingly being recognized as human pathogens. Relatively little is known, however, about the virulence attributes associated with C. tropicalis. The present study aimed to investigate epithelial infection by C. tropicalis using a reconstituted human oral epithelium (RHOE) together with confocal laser scanning microscopy and real-time PCR. A comparison of clinical strains was made in terms of tissue colonization, invasion and C. tropicalis secreted aspartyl proteinase (SAPT) gene expression. All C. tropicalis strains were able to colonize RHOE in a strain-dependent manner. After 12 h of infection, C. tropicalis was found to be highly invasive, with extensive tissue damage occurring after 24 h. Real-time PCR of C. tropicalis SAPT1-4 genes showed that expression was strain-dependent, with SAPT2-4 transcripts being frequently detected and SAPT1 rarely detected. Tissue invasion and damage was not inhibited by the presence of pepstatin A. Accordingly, and given that an increase in infection time was not accompanied with an increase in SAPT gene expression, it can be suggested that the proteinases are not involved in invasion and damage of RHOE by C. tropicalis. In summary, C. tropicalis can be considered as highly invasive with the ability to induce significant tissue damage. These features, however, do not appear to be related to specific SAPT gene expression.
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spelling The role of secreted aspartyl proteinases in Candida tropicalis invasion and damage of oral mucosaCandida tropicaliscandidosishuman epitheliumsecreted aspartyl proteinasesScience & TechnologyCandida virulence attributes include the ability to colonize and invade host tissues, and the secretion of hydrolytic enzymes. Although Candida albicans is regarded as the principal fungi causing infections in humans, other species, particularly Candida tropicalis, are increasingly being recognized as human pathogens. Relatively little is known, however, about the virulence attributes associated with C. tropicalis. The present study aimed to investigate epithelial infection by C. tropicalis using a reconstituted human oral epithelium (RHOE) together with confocal laser scanning microscopy and real-time PCR. A comparison of clinical strains was made in terms of tissue colonization, invasion and C. tropicalis secreted aspartyl proteinase (SAPT) gene expression. All C. tropicalis strains were able to colonize RHOE in a strain-dependent manner. After 12 h of infection, C. tropicalis was found to be highly invasive, with extensive tissue damage occurring after 24 h. Real-time PCR of C. tropicalis SAPT1-4 genes showed that expression was strain-dependent, with SAPT2-4 transcripts being frequently detected and SAPT1 rarely detected. Tissue invasion and damage was not inhibited by the presence of pepstatin A. Accordingly, and given that an increase in infection time was not accompanied with an increase in SAPT gene expression, it can be suggested that the proteinases are not involved in invasion and damage of RHOE by C. tropicalis. In summary, C. tropicalis can be considered as highly invasive with the ability to induce significant tissue damage. These features, however, do not appear to be related to specific SAPT gene expression.We would like to thank Mrs Kath Allsopp for processing and sectioning the tissue samples. This work was supported by grant SFRH/BD/28341/2006 from 'Fundacao para a Ciencia e Tecnologia' (FCT), Portugal.Blackwell Publishing Inc.Universidade do MinhoSilva, Sónia CarinaHooper, S. J.Henriques, MarianaOliveira, RosárioAzeredo, JoanaWilliams, D. W.20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/22710eng1198-743X10.1111/j.1469-0691.2010.03248.x20456460http://dx.doi.org/10.1111/j.1469-0691.2010.03248.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:04:56Zoai:repositorium.sdum.uminho.pt:1822/22710Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:55:15.753370Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The role of secreted aspartyl proteinases in Candida tropicalis invasion and damage of oral mucosa
title The role of secreted aspartyl proteinases in Candida tropicalis invasion and damage of oral mucosa
spellingShingle The role of secreted aspartyl proteinases in Candida tropicalis invasion and damage of oral mucosa
Silva, Sónia Carina
Candida tropicalis
candidosis
human epithelium
secreted aspartyl proteinases
Science & Technology
title_short The role of secreted aspartyl proteinases in Candida tropicalis invasion and damage of oral mucosa
title_full The role of secreted aspartyl proteinases in Candida tropicalis invasion and damage of oral mucosa
title_fullStr The role of secreted aspartyl proteinases in Candida tropicalis invasion and damage of oral mucosa
title_full_unstemmed The role of secreted aspartyl proteinases in Candida tropicalis invasion and damage of oral mucosa
title_sort The role of secreted aspartyl proteinases in Candida tropicalis invasion and damage of oral mucosa
author Silva, Sónia Carina
author_facet Silva, Sónia Carina
Hooper, S. J.
Henriques, Mariana
Oliveira, Rosário
Azeredo, Joana
Williams, D. W.
author_role author
author2 Hooper, S. J.
Henriques, Mariana
Oliveira, Rosário
Azeredo, Joana
Williams, D. W.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Silva, Sónia Carina
Hooper, S. J.
Henriques, Mariana
Oliveira, Rosário
Azeredo, Joana
Williams, D. W.
dc.subject.por.fl_str_mv Candida tropicalis
candidosis
human epithelium
secreted aspartyl proteinases
Science & Technology
topic Candida tropicalis
candidosis
human epithelium
secreted aspartyl proteinases
Science & Technology
description Candida virulence attributes include the ability to colonize and invade host tissues, and the secretion of hydrolytic enzymes. Although Candida albicans is regarded as the principal fungi causing infections in humans, other species, particularly Candida tropicalis, are increasingly being recognized as human pathogens. Relatively little is known, however, about the virulence attributes associated with C. tropicalis. The present study aimed to investigate epithelial infection by C. tropicalis using a reconstituted human oral epithelium (RHOE) together with confocal laser scanning microscopy and real-time PCR. A comparison of clinical strains was made in terms of tissue colonization, invasion and C. tropicalis secreted aspartyl proteinase (SAPT) gene expression. All C. tropicalis strains were able to colonize RHOE in a strain-dependent manner. After 12 h of infection, C. tropicalis was found to be highly invasive, with extensive tissue damage occurring after 24 h. Real-time PCR of C. tropicalis SAPT1-4 genes showed that expression was strain-dependent, with SAPT2-4 transcripts being frequently detected and SAPT1 rarely detected. Tissue invasion and damage was not inhibited by the presence of pepstatin A. Accordingly, and given that an increase in infection time was not accompanied with an increase in SAPT gene expression, it can be suggested that the proteinases are not involved in invasion and damage of RHOE by C. tropicalis. In summary, C. tropicalis can be considered as highly invasive with the ability to induce significant tissue damage. These features, however, do not appear to be related to specific SAPT gene expression.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/22710
url http://hdl.handle.net/1822/22710
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1198-743X
10.1111/j.1469-0691.2010.03248.x
20456460
http://dx.doi.org/10.1111/j.1469-0691.2010.03248.x
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing Inc.
publisher.none.fl_str_mv Blackwell Publishing Inc.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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