Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides

Detalhes bibliográficos
Autor(a) principal: Freire, João Miguel
Data de Publicação: 2015
Outros Autores: Dias, Susana Almeida, Flores, Luís, Veiga, Ana Salomé, Castanho, Miguel A. R. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/23081
Resumo: © The Author (2015). Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com
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spelling Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides© The Author (2015). Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.comSupplementary data are available at http://bioinformatics.oxfordjournals.org/lookup/suppl/doi:10.1093/bioinformatics/btv131/-/DC1Motivation: The need for more effective and safer pharmaceuticals is a persistent quest. Microbial adaptations create the need to permanently develop new antimicrobials, for instance. Similarly, intracellular delivery of drugs is still a challenge and translocation of membranes for drug delivery is an area of intense research. Peptides can be used both as antimicrobial drug leads and drug carrier systems for intracellular delivery. Multifunctional proteins are abundant in viruses but, surprisingly, have never been thoroughly screened for bioactive peptide sequences. Results: Using the AMPA and CellPPD online tools we have evaluated the propensity of viral proteins to comprise antimicrobial (AMP) or cell-penetrating peptides (CPP). Capsid proteins from both enveloped and non-enveloped viruses, and membrane and envelope proteins from enveloped viruses, in a total of 272 proteins from 133 viruses, were screened to detect the presence of potential AMP and CPP sequences. A pool of 2444 and 426 CPP and AMP sequences, respectively, were discovered. The capsids of flaviviruses are the best sources of these peptides reaching more than 80% of CPP sequence coverage per protein. Selected sequences were tested experimentally and validated the results. Overall, this study reveals that viruses form a natural multivalent biotechnological platform still underexplored in drug discovery and the heterogeneous abundance of CPP/AMP sequences among viral families opens new avenues in viral biology research.JMF and ASV acknowledge FCT-MEC for PhD fellowship SFRH/BD/70423/2010 and fellowship F/00803/2012 under the FCT Investigator Programme. Funding: Fundação para a Ciência e Tecnologia – Ministério da Educação e Ciência (FCT-MEC, Portugal) [HIVERA/0002/2013].Oxford University PressRepositório da Universidade de LisboaFreire, João MiguelDias, Susana AlmeidaFlores, LuísVeiga, Ana SaloméCastanho, Miguel A. R. B.2016-03-18T15:01:49Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/23081engBioinformatics (2015) , 31 (14): 2252-22561367-480310.1093/bioinformatics/btv131metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:10:53Zoai:repositorio.ul.pt:10451/23081Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:40:33.627571Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides
title Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides
spellingShingle Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides
Freire, João Miguel
title_short Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides
title_full Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides
title_fullStr Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides
title_full_unstemmed Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides
title_sort Mining viral proteins for antimicrobial and cell-penetrating drug delivery peptides
author Freire, João Miguel
author_facet Freire, João Miguel
Dias, Susana Almeida
Flores, Luís
Veiga, Ana Salomé
Castanho, Miguel A. R. B.
author_role author
author2 Dias, Susana Almeida
Flores, Luís
Veiga, Ana Salomé
Castanho, Miguel A. R. B.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Freire, João Miguel
Dias, Susana Almeida
Flores, Luís
Veiga, Ana Salomé
Castanho, Miguel A. R. B.
description © The Author (2015). Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2016-03-18T15:01:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/23081
url http://hdl.handle.net/10451/23081
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bioinformatics (2015) , 31 (14): 2252-2256
1367-4803
10.1093/bioinformatics/btv131
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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