Human gingival fibroblast and osteoblast behavior on groove-milled zirconia implant surfaces

Detalhes bibliográficos
Autor(a) principal: Brito da Cruz, Mariana
Data de Publicação: 2022
Outros Autores: Marques, Joana, Silva, Neusa, Madeira, Sara, Carvalho, Óscar, Silva, Filipe Samuel, Caramês, João, Mata, António
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/55291
Resumo: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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spelling Human gingival fibroblast and osteoblast behavior on groove-milled zirconia implant surfacesDental implantsFibroblastsMillingOsteoblastsZirconia© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Two type of cells representing periodontal hard tissues (osteoblasts) and soft tissues (fibroblasts) were evaluated in response to microgroove-milled zirconia surfaces. A total of 90 zirconia discs were randomly assigned to four width-standardized milling microgroove-textured groups and a control group without grooves (UT). The sandblast and acid-etch protocol were applied to all samples. Both cell lines were cultured on zirconia discs from 1 day up to 14 days. Cell morphology and adhesion were evaluated after 1 day of culturing. Cell viability and proliferation of the cells were measured. Alkaline phosphatase activity, collagen I, osteopontin, interleukin 1β and interleukin 8 secretions were assessed at predefined times. The results obtained were presented in the form of bar graphs as means and standard deviations. Multi comparisons between groups were evaluated using two-away ANOVA or Mann-Whitney tests, and a p-value < 0.05 was established. Group comparisons with regard to cell viability, proliferation and secretion of collagen I, interleukin-1β and interleukin 8 revealed no statistically significant differences. The alkaline phosphatase activity and osteopontin secretion were significantly higher in the group with a large groove compared to the small one and the control group. Nevertheless, the viability of gingival and bone cells did not appear to be affected by the milled microgroove texture compared to the conventional sandblasted and acid-etched texture, but they seem to influence osteoblasts' cellular differentiation.This research was supported by FCT project POCI-01-0145-FEDER-030498—Portugal, by FEDER funds through the COMPETE 2020—Programa Operacional Competitividade e Internacionalização (POCI) and ImpHib–Development of advanced Hybrid Implants—NORTE-01-0247-FEDER-034074.MDPIRepositório da Universidade de LisboaBrito da Cruz, MarianaMarques, JoanaSilva, NeusaMadeira, SaraCarvalho, ÓscarSilva, Filipe SamuelCaramês, JoãoMata, António2022-11-29T16:19:52Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/55291engMaterials (Basel). 2022 Mar 28;15(7):24811996-194410.3390/ma15072481info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:02:09Zoai:repositorio.ul.pt:10451/55291Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:05:58.147563Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Human gingival fibroblast and osteoblast behavior on groove-milled zirconia implant surfaces
title Human gingival fibroblast and osteoblast behavior on groove-milled zirconia implant surfaces
spellingShingle Human gingival fibroblast and osteoblast behavior on groove-milled zirconia implant surfaces
Brito da Cruz, Mariana
Dental implants
Fibroblasts
Milling
Osteoblasts
Zirconia
title_short Human gingival fibroblast and osteoblast behavior on groove-milled zirconia implant surfaces
title_full Human gingival fibroblast and osteoblast behavior on groove-milled zirconia implant surfaces
title_fullStr Human gingival fibroblast and osteoblast behavior on groove-milled zirconia implant surfaces
title_full_unstemmed Human gingival fibroblast and osteoblast behavior on groove-milled zirconia implant surfaces
title_sort Human gingival fibroblast and osteoblast behavior on groove-milled zirconia implant surfaces
author Brito da Cruz, Mariana
author_facet Brito da Cruz, Mariana
Marques, Joana
Silva, Neusa
Madeira, Sara
Carvalho, Óscar
Silva, Filipe Samuel
Caramês, João
Mata, António
author_role author
author2 Marques, Joana
Silva, Neusa
Madeira, Sara
Carvalho, Óscar
Silva, Filipe Samuel
Caramês, João
Mata, António
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Brito da Cruz, Mariana
Marques, Joana
Silva, Neusa
Madeira, Sara
Carvalho, Óscar
Silva, Filipe Samuel
Caramês, João
Mata, António
dc.subject.por.fl_str_mv Dental implants
Fibroblasts
Milling
Osteoblasts
Zirconia
topic Dental implants
Fibroblasts
Milling
Osteoblasts
Zirconia
description © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
publishDate 2022
dc.date.none.fl_str_mv 2022-11-29T16:19:52Z
2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/55291
url http://hdl.handle.net/10451/55291
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Materials (Basel). 2022 Mar 28;15(7):2481
1996-1944
10.3390/ma15072481
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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