Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2009 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/5855 |
Resumo: | PLos One, 4(11): ARTe7722 |
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Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidisACME-ARCA GENE RESISTANCETYPING METHODSAUREUSSEQUENCECLONEIDENTIFICATIONCOMBINATIONVIRULENCEUSA300PLos One, 4(11): ARTe7722Background: The methicillin-resistant Staphylococcus aureus clone USA300 contains a novel mobile genetic element, arginine catabolic mobile element ( ACME), that contributes to its enhanced capacity to grow and survive within the host. Although ACME appears to have been transferred into USA300 from S. epidermidis, the genetic diversity of ACME in the latter species remains poorly characterized. Methodology/Principal Findings: To assess the prevalence and genetic diversity of ACME, 127 geographically diverse S. epidermidis isolates representing 86 different multilocus sequence types (STs) were characterized. ACME was found in 51% (65/127) of S. epidermidis isolates. The vast majority (57/65) of ACME-containing isolates belonged to the predominant S. epidermidis clonal complex CC2. ACME was often found in association with different allotypes of staphylococcal chromosome cassette mec (SCCmec) which also encodes the recombinase function that facilities mobilization ACME from the S. epidermidis chromosome. Restriction fragment length polymorphism, PCR scanning and DNA sequencing allowed for identification of 39 distinct ACME genetic variants that differ from one another in gene content, thereby revealing a hitherto uncharacterized genetic diversity within ACME. All but one ACME variants were represented by a single S. epidermidis isolate; the singular variant, termed ACME-I.02, was found in 27 isolates, all of which belonged to the CC2 lineage. An evolutionary model constructed based on the eBURST algorithm revealed that ACME-I.02 was acquired at least on 15 different occasions by strains belonging to the CC2 lineage. Conclusions/Significance: ACME-I.02 in diverse S. epidermidis isolates were nearly identical in sequence to the prototypical ACME found in USA300 MRSA clone, providing further evidence for the interspecies transfer of ACME from S. epidermidis into USA300.Public Library of ScienceRUNMiragaia, Mde Lencastre, HPerdreau-Remington, FChambers, HFHigashi, JSullam, PMLin, JWong, KIKing, KAOtto, MSensabaugh, GFDiep, BA2011-06-27T14:26:15Z2009-01-01T00:00:00Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/5855eng1932-6203info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-10T15:25:42ZPortal AgregadorONG |
| dc.title.none.fl_str_mv |
Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis |
| title |
Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis |
| spellingShingle |
Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis Miragaia, M ACME-ARCA GENE RESISTANCE TYPING METHODS AUREUS SEQUENCE CLONE IDENTIFICATION COMBINATION VIRULENCE USA300 |
| title_short |
Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis |
| title_full |
Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis |
| title_fullStr |
Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis |
| title_full_unstemmed |
Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis |
| title_sort |
Genetic diversity of arginine catabolic mobile element in Staphylococcus epidermidis |
| author |
Miragaia, M |
| author_facet |
Miragaia, M de Lencastre, H Perdreau-Remington, F Chambers, HF Higashi, J Sullam, PM Lin, J Wong, KI King, KA Otto, M Sensabaugh, GF Diep, BA |
| author_role |
author |
| author2 |
de Lencastre, H Perdreau-Remington, F Chambers, HF Higashi, J Sullam, PM Lin, J Wong, KI King, KA Otto, M Sensabaugh, GF Diep, BA |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
RUN |
| dc.contributor.author.fl_str_mv |
Miragaia, M de Lencastre, H Perdreau-Remington, F Chambers, HF Higashi, J Sullam, PM Lin, J Wong, KI King, KA Otto, M Sensabaugh, GF Diep, BA |
| dc.subject.por.fl_str_mv |
ACME-ARCA GENE RESISTANCE TYPING METHODS AUREUS SEQUENCE CLONE IDENTIFICATION COMBINATION VIRULENCE USA300 |
| topic |
ACME-ARCA GENE RESISTANCE TYPING METHODS AUREUS SEQUENCE CLONE IDENTIFICATION COMBINATION VIRULENCE USA300 |
| description |
PLos One, 4(11): ARTe7722 |
| publishDate |
2009 |
| dc.date.none.fl_str_mv |
2009-01-01T00:00:00Z 2009-01-01 2011-06-27T14:26:15Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/5855 |
| url |
http://hdl.handle.net/10362/5855 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1932-6203 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Public Library of Science |
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Public Library of Science |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777302878834130944 |